Assessment of genetic diversity in Ganoderma lucidum using RAPD and ISSR markers

Authors

  • RENU THAKUR, POOJA KAPOOR*, PREM NATH SHARMA and BRIJ MOHAN SHARMA

Keywords:

Ganoderma lucidum, genetic variability, RAPD, ISSR

Abstract

Genetic diversity of 31 isolates of Ganoderma lucidum from different hosts and geographic regions were analyzed using RAPD and ISSR markers. Ten polymorphic RAPDs were selected from 156 primers along with 8 polymorphic universal ISSR primers. RAPD and ISSR exhibited polymorphism of 88.2 and 78.8 per cent, respectively. RAPD markers, viz. S-1005, OPF-6 exhibited maximum polymorphism (93.3%) whereas ISSR- 833 showed maximum of 89.6 per cent polymorphism. Cluster analysis of 31 isolates/strains of G. lucidum grouped isolates in 4 subpopulation at 42 per cent (RAPD) and 5 subpopulation using ISSR markers at 40 per cent similarity coefficient. Multilocus ISSR primers clustered isolates based on their geographical origin to considerable levels and exhibited fair similarities among grouping of isolates having sessile/short stalk length than those having long stalk. Therefore, this study reports the high level of genetic diversity of Ganoderma lucidum from North Western Himalayas using both RAPD and ISSR techniques.

Downloads

Issue

Vol. 68 No. 3 (2015): Indian Phytopathology

Section

Research Articles

License

For Authors

As soon as an article is accepted for publication, authors are requested to assign copyright of the article (or to grant exclusive publication and dissemination rights) to the publisher (Indian Phytopathlogical Society). This will ensure the widest possible protection and dissemination of information.

For Readers

While the advice and information in this journal is believed to be true and accurate at the date of its publication, the authors, the editors, nor the publisher can accept any legal responsibility for any errors or omissions that may have been made. The publisher makes no warranty, express or implied, with respect to the material contained herein.

All articles published in this journal are protected by copyright, which covers the exclusive rights to reproduce and distribute the article (e.g., as offprints), as well as all translation rights. No material published in this journal may be reproduced photographically or stored on microfilm, in electronic data bases, on video disks, etc., without first obtaining written permission from the publisher. The use of general descriptive names, trade names, trademarks, etc. in this publication, even if not specifically identified, implies that these names are protected by the relevant laws and regulations.

How to Cite

PREM NATH SHARMA and BRIJ MOHAN SHARMA, R. T. P. K. (2015). Assessment of genetic diversity in Ganoderma lucidum using RAPD and ISSR markers. Indian Phytopathology, 68(3), 316-320. http://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/49846