Molecular identification of a new strain Narcissus yellow stripe virus strain associated with severe mosaic disease of Narcissus from India

Authors

  • NEELAM YADAV* and JAWAID A. KHAN

Abstract

A Potyvirus associated with severe mosaic disease of Narcissus tazetta in India was characterized at molecular level. Nucleotide sequence of its 3΄-terminal genome (~2900 bp) comprising 3΄-non translated region (NTR), large nuclear inclusion protein (NIb, RdRp, replicase), coat protein (CP) and C-terminus of NIa-proteinase (NIa-Pro) showed 93% and 85% homology whereas, CP gene showed 97% and 87% identities with Narcissus yellow stripe virus (NYSV) at amino acid and nucleotide level, respectively. 3΄-NTR showed maximum homology of 98% with NYSV.  Phylogenetic analyses of present viral genome showed its close relatedness to NYSV, at nucleotide and amino acid level using CP, NIb and 3΄-NTR. Thus, it can be clearly concluded that it is a new strain of Narcissus yellow stripe virus from India.

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Vol. 68 No. 4 (2015): Indian Phytopathology

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Research Articles

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How to Cite

JAWAID A. KHAN, N. Y. and. (2015). Molecular identification of a new strain Narcissus yellow stripe virus strain associated with severe mosaic disease of Narcissus from India. Indian Phytopathology, 68(4). http://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/54627