Assessment of molecular diversity among Pearl Millet (Pennisetum glaucum (L.) R. Br.) Maintainer (B) and Restorer (R) lines.
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Molecular diversity in pearl millet
Pearl millet is predominantly cultivated in western arid and semi-arid region. Pearl millet productivity has drastically increased in the seven decades mainly due to adoption of hybrid varieties in large. Hybrid development programme mainly depends upon exploitation of diverse genetic stock. In the present study a set of 48 maintainer line (B) and 52 restorer ® parent were assessed for molecular diversity using PCR based RAPD and ISSR markers. The Jaccard's similarity coefficient was used to create pair-wise comparisons of distinct and shared polymorphic amplification products. The dendrogram represented the classification of all 100-pearl millet genotypes into two major groups. First group was formed with single genotype J-2296 and second group consisted of 99 genotypes further grouped in three sub-clusters. In second major cluster, maximum genotypes were grouped in subcluster I (45) followed by subcluster II (35) and subcluster III (19). Grouping of B lines and R lines were random no specific grouping was observed. The observed divergence can be useful for selecting B lines and R lines from different clusters for developing hybrids. J-2296 seems to be highly diverse from rest of the genotypes, it can be used for hybrid development as well as for R line improvement.
Doyle, J.J. and Doyle, J.L. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13-15.
Fahima, T., Sun, G.L., Beharav, A., Krugman, T., Beiles, A. and Nevo, E. 1999. RAPD polymorphism of wild emmer wheat populations, Triticum dicoccoides, in Israel. Theoretical and Applied Genetics 98: 43-47.
Hash, C.T., Bhasker, A.G. and Lineup, S. 2003. Opportunities for marker-assisted selection (MAS) to improve the feed quality of crop residues in pearl millet and sorghum. Field Crops Research 84: 79-88.
Jambunathan, R. and Subramanian, V. 1988. Grain quality and utilisation in sorghum and pearl millet. In: Workshop on Biotechnology for Tropical Crop Improvement, ICRISAT, Patancheru. 13301339.
Jaya Prakash, S.P., Biji, K.R., Michael Gomez, S., Ganesa Murthy, K, and Chandra Babu, R. 2006. Genetic diversity analysis of sorghum (Sorghum bicolor L. Moench) accessions using RAPD markers. Indian Journal of Crop Sciences 1(1-2): 109-112.
Jukanti, A.K. Gowda, C.L.L., Rai, K.N., Manga, V.K. and Bhatt, R.K. 2016. Crops that feed the world 11. Pearl millet (Pennisetum glaucum L.): An important source of food security, nutrition and health in the arid and semi-arid tropics. Food Science 8: 307-329.
Liu, C.J, Witcombe, J.R., Hash, C.T., Busso, C.S., Pittaway, T.S., Nash, M. and Gale, M.D. 1994. Use of Molecular Marker in Sorghum and Pearl Millet Breeding for Developing Countries. (Eds. J.R. Witcombe and R.R. Duncan): Overseas Development Administration: London U.K. pp. 57-69.
Pandey, P., Pandey, V.R., Kumar, A., Yadav, S., Tiwari, D. and Kumar, R. 2015. Relationship between heterosis and genetic diversity in Indian pigeon pea [Cajanus cajan (L.) Millspaugh] accessions using multivariate cluster analysis and heterotic grouping. Australian Journal of Crop Science 9(6): 494-503.
Passoupathy, R., Krishna, H. and Nadoor, S. 2015. Prediction of heterosis in crop plants – Status and prospects. American Journal of Experimental Agriculture 9(3): 1-16.
Rai, K.N., Gowda, C.L.L., Reddy, B.V.S. and Sehgal, S. 2008. Adaptation and potential use of sorghum and pearl millet in alternative and health foods. Compressive Reviews in Food Science and Food Safety 7: 340-352.
Rani, K.J. and Rao, S.S. 2009. Relationship between heterosis and genetic divergence in rabi sorghum [Sorghum bicolor (L.) Moench]. Research on Crops 10: 319-322.
Rohlf, F.J. 1993. NTSYS-pc Numerical Taxonomy and Multivariate System, Version 1.8. Applied Biostatistics, New York, USA
Rooney, L.W. and McDonough, C.M. 1987. Food quality and consumer acceptance in pearl millet. In: Proceedings of the International Pearl Millet Workshop (Eds. J.R. Witcombe and S.R. Beckerman), April 7-11, 1986, ICRISAT, Patancheru. pp. 43-61.
Satyavathi, C.T., Tomar, R.S, Ambawat, S., Kheni, J., Padhiyar, S.M., Desai, H., Bhatt, S.B, Shitap, M.S., Meena, R.C., Singhal, T., Sankar, S.M., Singh, S.P. and Khandelwal, V. 2022. Stage specific comparative transcriptomic analysis to reveal gene networks regulating iron and zinc content in pearl millet [Pennisetum glaucum (L.) R. Br.]. Science Reporter 12: 276. 10.1038/s41598021-04388-0
Satyavathi, C.T., Solanki, R., Kakani, R., Bharadwaj, C., Singhal, T., Padaria, J., Khandelwal, V., Srivastava, R., Tomar, R. and Iqubal, M. 2019. Genomics assisted breeding for abiotic stress tolerance in Millets. In: Genomics Assisted Breeding of Crops for Abiotic Stress Tolerance, Vol. II”. (Eds. V.R. Rajpal, D. Sehgal, A. Kumar and S.N. Raina) Springer Nature Switzerland. pp. 241-255.
Sharma, A. and Choudhary, M. 2012. Development and quality evaluation of pearl millet based Anti-diabetic flour. Annals of Arid Zone 51(2): 105-108.
Tomar, R.S, Parakhia, M.V., Rathod, V.M., Thakkar, J.R. and Golakiya, B.A. 2014a. A Comparative analysis of ISSR and RAPD markers for studying genetic diversity in Trigonella foenum-graecum genotypes. Research Journal on Biotechnology 9: 89-95.
Tomar, R.S., Kulkarni, G.U., Parakhia, M.V., Thakkar, J.R., Rathod, V.M., Solanki, R.K. and Golakiya, B.A. 2014b. Genetic diversity analysis in coriander (Coriandrum sativum L.) genotypes through morphological and molecular characterization. Research Journal on Biotechnology 9: 1-11
Yadav, O.P., Rai, K.N. and Gupta, S.K. 2012. Pearl millet: Genetic improvement for tolerance to abiotic stresses. In: Improving Crop Resistance to Abiotic Stress. Improving Crop Productivity in Sustainable Agriculture. First edn. (Eds. N. Tuteja, S.S. Gill and R. Tuteja). Wiley VCH Verlag GmbH & Co KGaA, Weinheim, pp. 261-288.
Yadav, O.P., Gupta, S.K., Mahalingam, G., Sharma, R, Varshney, R.K., Srivastava R.K., Rathore, A. and Mahala, R.S. 2021. Genetic gains in pearl millet in India: Insights into historic breeding strategies and future perspective. Frontiers in Plant Science. 12: 645038.
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