Amelioration of Salinity Stress and Enhancing Growth in Wheat Seedling using Halotolerant Arthrobacter sp. NIMD28

Kamlesh Kumar  Meena; Devendra Singh; Saritha M; Priyanka Verma; Pooja Gahlot; Dheeraj Singh

doi:10.56093/aaz.v64i3.167571

Authors

Kamlesh Kumar Meena Principal Scientist, ICAR-Central Arid Zone Research Institute, Jodhpur 342 003, India
Devendra Singh Scientist, ICAR-Central Arid Zone Research Institute, Jodhpur 342 003, India
Saritha M Scientist, ICAR-Central Arid Zone Research Institute, Jodhpur 342 003, India
Priyanka Verma ICAR-Central Arid Zone Research Institute, Jodhpur 342 003, India
Pooja Gahlot ICAR-Central Arid Zone Research Institute, Jodhpur 342 003, India
Dheeraj Singh Principal Scientist, ICAR-Central Arid Zone Research Institute, Jodhpur 342 003, India

https://doi.org/10.56093/aaz.v64i3.167571

Keywords:

Arthrobacter sp., Bacterial Metabolites, Halotolerant rhizobacteria, Salinity stress, induced systemic tolerance

Abstract

Growth promotion and salinity stress-attenuation ability of the metabolites secreted by plant growth promoting, halotolerant Arthrobacter sp. NIMD28 (LC189176) was evaluated on germination, establishment, and development of wheat. The isolate produced diverse types of biomolecules in varying concentrations. Metabolites produced by the isolate also showed presence of phenolic compounds with significant non-enzymatic antioxidant activity. The gnotobiotic seed germination experiment revealed interesting results, where despite of the halotolerant nature, the isolate seems lagged in the plant growth promotion (PGP) over the in vitro induced metabolites of its own in terms of the phenotypic, biochemical and enzymatic characters. The Pearson’s correlation coefficient and hierarchical clustering analyses also endorsed the performance of metabolites under saline conditions. The results strongly endorse needful initiatives towards keen optimization of the dose-response of the metabolites at larger scale, to develop next generation, sustainable bio-inoculants for abiotically stressed habitats.

Downloads

Download data is not yet available.

References

Aebi, H.1984. Catalase in vitro. Methods in Enzymology 105:121-126.

Al-Wakeel, S.M., Gabr, M.M., Abu-El-Soud, W.M., Saleh, A.M. and Abu El-Soud, W.M. 2013. Coumarin and salicylic acid activate resistance to Macrophomina phaseolina in Helianthus annuus. Acta Agronomica Hungarica 61: 23-35.

Bashan, Y., Kamnev, A.A. and de Bashan, L.E. 2013. A proposal for isolating and testing phosphate solubilizingbacteria that enhance plant growth. Biology and Fertility of Soils 49:1–2.

Beauchamp, C. and Fridovich, I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical

Biochemistry 44: 276-287.

Bharti N, Yadav D, Barnawal D, Maji D, Kalra A. 2013. Exiguobacterium oxidotolerans a halotolerant plant growth promoting rhizobacteria, improves yield and content of secondary metabolites in Bacopamonnieri (L.) Pennell under primary and secondary salt stress. World Journal of Microbiology & Biotechnology 29: 379-387.

Bose, J., Moreno, A.R. and Shabala, S. 2014. ROS homeostasis in halophytes in the context of salinity stress tolerance. Journal of Experimental Botany 65: 1241-1257.

Bray, H.G. and Thorpe, W.V.1954. Analysis of phenolic compounds of interest in metabolism. Methamphetamine Biochemistry and Analysis 1: 27-52.

Chaitanya, K.S.K. and Naithani, S.C. 1994. Role of superoxide, lipid peroxidation and superoxide dismutase in membrane perturbation during loss of viability in seeds of Shorea robusta. The New Phytologist is New Phytol 126: 623-627.

Chookietwattana, K. and Maneewan, K. 2012. Selection of efficient salt-tolerant bacteria containing ACC deaminase for promotion

of tomato growth under salinity stress. Soil Environment 31: 30-36

Corwin, D.L. and Lesch, S.M. 2005. Apparent soil electrical conductivity measurements in agriculture. Computers and Electronics in

Agriculture 46:11-43.

Damerum, A., Selmes, S.L., Biggi, G.F., Clarkson, G.J., Rothwell, S.D., Truco, M.J., Michelmore, R.W., Hancock, R.D., Shellcock, C., Chapman, M.A. and Tylor, G. 2015. Elucidating the genetic basis of antioxidant status in lettuce (Lactuca sativa). Horticulture Research https://doi.org/10.1038/hortres.2015.55 PMID: 26640696

De Pinto, M.C., Locato, V. and De Gara, L. 2012. Redox regulation in plant programmed cell death. Plant Cell & Environment 35:234-244.

Ehmann, A. 1977. The Van Urk-Salkowski reagent-a sensitive and specific chromogenic reagent for silica gel thin-layer chromatographic detection and identification of indole derivatives. Journal of Chromatography 132: 267-276.

Eyidogan, F. and Oz, M.T. 2005. Effect of salinity on antioxidant responses of chickpea seedlings, Acta Physiologiae Plantarum 29: 485-493.

Fujishige, N.A., Kapadia, N.N. and Hirsch, A.M. 2006. A feeling for the micro-organism: structure on a small scale. Biofilms on plant roots. Botanical Journal of the Linnean Society 150: 79-88.

Habib, S.H., Kausar, H. and Saud, H.M. 2016. Plant Growth-Promoting Rhizobacteria Enhance Salinity Stress Tolerance in Okra through ROSScavenging Enzymes. Hindawi Publishing Corporation. BioMed Research International Article ID 6284547 1-10.

Harborne, J.B. 1973. Phytochemical Methods. Chapman and Hall, Ltd., London 49:188.

Hasanuzzaman, M., Nahar, K. and Fujita, M. 2013. Plant response to salt stress and role of exogenous protectants to mitigate salt induced damages. In: Ahmad, P., Azooz, M., Prasad, M. (eds) Ecophysiology and Responses of Plants under Salt Stress. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-4747-4_2.

Kerepesi, I. and Galiba, G. 2000. Osmotic and salt stress-induced alteration in soluble carbohydrate content in wheat seedlings. Crop Science 40: 482- 487.

Khan, M.A. and Webber, D.J. 2008. Ecophysiology of High Salinity Tolerant Plants (tasks for vegetation science), Ist Edn. Springer, Amsterdam ISBN 978- 1-4020-9298-5.

Lim, J.H., Park, K.J., Kim, B.K., Jeong, J.W. and Kim, H.J. 2012. Effect of salinity stress on phenolic compounds and carotenoids in buckwheat (Fagopyrum esculentum M.) sprout. Food Chem 135:1065-1070.

Lowry, O.H., Rosenbury, N.J., Farr, A.L. and Randall, R.J. 1951. Protein measurement with folin phenol reagent. Journal of Biological Chemistry 193: 262-275.

Luck, H. 1974. Estimation of catalase. In: Methods in Enzymatic Analysis. (Ed. H. Bergmeyer), pp.885. Academic Press, New York.

Munnus, R. 2002. Salinity, growth and phytohormones. In: Salinity: Environment-Plants-Molecules (Eds. A. Lauchli and U. Luttge), pp.

271-90. Kluwer, The Netherlands.

Nautiyal, C.S. 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters 170: 265-270.

Patil, A.D. 2015. Alleviating Salt Stress in Crop Plants through Salt Tolerant Microbes. International Journal of Science and Research (IJSR) 1297-1302.

Puniran-Hartley, N., Hartley, J., Shabala, L. and Shabala, S. 2014. Salinity-induced accumulation of organic osmolytes in barley and wheat leaves correlates with increased oxidative stress tolerance: in planta evidence for cross-tolerance.

Plant Physiology and Biochemistry 83: 32-39.

Putter, J. 1974. Peroxidase In: Methods of Enzymatic Analysis (Ed. H.U. Bergmeyer) Weinhan. Verlag Chemie 2: 685-690.

Qureshi, M.I., Isra, M., Abdin, M.Z. and Iqbal, M. 2005. Responses of Artemisia annua L., to lead salt induced oxidative stress. Environmental and Experimental Botany 53: 185-193.

Ramadoss, D., Lakkineni, V.K., Bose, P., Ali, S. and Annapurna, K. 2013. Mitigation of salt stress in wheat seedlings by halotolerant bacteria isolated from saline habitats. Springer Plus 6: 1-7.

Reddy, K., Subhani, S., Khan, P. and Kumar, K. 1995. Effect of light and benzyl adenine on dark-treated growing rice leaves: II changes in peroxidase activity. Plant Cell Physiology 24: 987- 994.

Reinhold, B., Hurek, T., Fendrik, I., Pot, B., Gillis, M., Kersters, K., Thielemans, S. and Ley, J.D. 1987. Azospirillum halopraeferens sp. nov., a nitrogen fixing organism associated with roots of Kallar grass (Leptochloafusca (L.) Kunth. International

Journal of Systematic Bacteriology 37: 43.

Reinhold, B., Hurek, T., Niemann, E.G. and Fendrik, L.1986. Close association of Azospirillum and diazotrophic rods with different root zones of Kallar grass. Applied and Environmental Microbiology 52: 520.

Schwyn, B. and Neilands, J.B.1987. Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry 160: 47–56.

Shrivastava, P. and Kumar, R. 2015. Soil salinity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Sciences 22: 123-131.

Siddikee, M.A., Chauhan, P.S., Anandham, R., Han, G.H. and Sa, T .2010. Isolation, characterization, and use for plant growth promotion under salt stress, of ACC Deaminase-Producing Halotolerant Bacteria Derived from Coastal Soil.

Journal of Microbiology and Biotechnology 1577- 1584.

Sorty, A.M., Meena, K.K., Choudhary, K., Bitla, U.M., Minhas, P.S. and Krishnani, K.K. 2016. Effect of plant growth gromoting bacteria associated with halophytic weed (Psoralea corylifolia L.) on germination and seedling growth of wheat under saline conditions. Applied Biochemistry and Biotechnology 180: 872-882.

Supanekar, S.V. and Sorty, A.M. 2013. Siderophoregenic Klebsiella pneumoniae SUP II from wheat (Triticum aestivum) rhizoplane.

Indian Journal of Research 2: 243-245.

Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30: 2725-2729.

Upadhyay, S.K., Singh, J.S., Saxena, A.K. and Singh, D.P. 2012. Impact of PGPR inoculation on growth and antioxidant status of wheat under saline conditions. Plant Biology 14: 605-611.

Vyride, I. and Stuckey, D.C. 2009. Adaptation of anaerobic biomass to saline conditions: Role of compatible solutes and extracellular

polysaccharides. Enzyme and Microbial Technology 44: 46-51.

Yen, G.C. and Duh, P.D. 1994. Scavenging effect of methanolic extracts of peanut hulls on free radical and active-oxygen species. Journal of Agricultural and Food Chemistry 42: 629-632.

Yuan, J., Zhang, N., Huang, Q., Raza, W., Li, R., Vivanco, J.M. and Shen, Q. 2015. Organic acids from root exudates of banana help

root colonization of PGPR strain Bacillus amyloliquefaciens NJN-6. Scientific Reports 24: 234-238.