Extraction and Functional Group Analysis of Gossypol from Cottonseed Meal

Abstract views: 218 / PDF downloads: 8


  • MANOJ KUMAR Scientist, ICAR - Central Institute for Research on Cotton Technology (CIRCOT), Mumbai-400019
  • JAYASHREE POTKULE Junior Research Fellow, ICAR - Central Institute for Research on Cotton Technology (CIRCOT), Mumbai-400019
  • SUJATA SAXENA Principal Scientist, ICAR - Central Institute for Research on Cotton Technology (CIRCOT), Mumbai-400019


Gossypol, Extraction, FTIR, Cottonseed meal


Gossypol is a polyphenolic aldehydic compound which has been studied for its versatile biological activities. The extraction of gossypol from cottonseed meal was attempted using acidified solvent system. Mixture of acetone: ethanol and water (70:20:10) at 4.5 pH with solid-to-liquid ratio of 1:20 can be considered as best conditions for the extraction of gossypol. The purity and presence of specific functional groups was confirmed using Fourier Transform Infrared (FTIR) spectroscopy. Study will give a basis to further optimize the protocol for efficient extraction of gossypol form cottonseed meal for possible applications in agriculture, medicine and pharmaceuticals.


Download data is not yet available.


Bellamy, L. J. (1975) - The Infrared Spectra of Complex Molecules (3rd Ed.), Chapman & Hall, London, pp.151–177.

Coutinho, E. M. (2002) - Gossypol: a contraceptive for men - Contraception, 65(4):259–263.

Dechary, J. M., Kupperman, R. P., Thurber, F. H., Altschul, A. M. and Loboratory, R. R. (1952) - Removal of gossypol from cottonseed by solvent extraction procedures - J. Am. Oil Chem. Soc., 29:339–341.

Gadelha, I. C., Fonseca, N. B., Oloris, S. C., Melo, M. M. and Soto-Blanco, B. (2014) - Gossypol toxicity from cottonseed products- Sci. World J., 231635.

Keshmiri-Neghab, H. and Goliaei, B. (2013) - Therapeutic potential of gossypol: An overview - Pharm.l Biol., 52(1):124–128.

Kuk, M. S., Tetlow, R. and Dowd, M. K. (2005)- Cottonseed extraction with mixtures of acetone and hexane - J. Am. Oil Chem. Soc., 82(8):609-612.

Kumar, M., Mageshwaran, V., Saxena, S., D’ Souza, C., Ashtaputre N., Patil S., Mahapatra A., Patil P. G. and Tak, Y. (2019) - Value addition of cottonseed meal for feed industries by degossypolization employing papain and solvent - Res. J. Biotechnol., 14 (11).

Kumar, M., Potkule, J., Saxena, S. and Patil, P. G. (2018) - Value-addition of cottonseed: Protein extraction, functional characterization and its application - Cotton Res. J., 9(1-2): 51-57

Li, Z., Smith, K. H. and Stevens, G. W. (2016) - The use of environmentally sustainable bio-derived solvents in solvent extraction applications—A review - Chin. J. Chem. Eng., 24: 215–220.

Mirghani, M. E. S. and Che Man, Y. B. (2003) - A new method for determining gossypol in cottonseed oil by FTIR spectroscopy - J. Am. Oil Chem. Soc.,80(7):625–628.

Nakanishi, K. and Solomon P. H. (1977) - Infrared Absorption Spectroscopy (2nd Ed.), Holden-Day, San Francisco, pp.1–5.

Pelitire, S. M., Dowd, M. K. and Cheng, H. N. (2014) - Acidic solvent extraction of gossypol from cottonseed meal - Animal Feed Sci. Technol., 195:120–128.

Singh, S., Sharma, S. K. and Kansal, S. K. (2015) - Extraction of Gossypol from Cottonseed -Review in Adv. Sci. Eng., 4:301–318.

Stuart, B. (1996) - Modern Infrared Spectroscopy, (D. J. Ando, ed.), John Wiley and Sons, New York, 135.






How to Cite

KUMAR, M., POTKULE, J., & SAXENA, S. (2021). Extraction and Functional Group Analysis of Gossypol from Cottonseed Meal. Cotton Research Journal, 10(1). Retrieved from https://epubs.icar.org.in/index.php/CoRJ/article/view/114037