Effect of Urea and Exogenous Fibrolytic Enzyme Treated Paddy Straw on In Vitro Rumen Fermentation Characteristics and Degradability
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Authors
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Naitam Shubham Devidas
Veterinary University, Mathura
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Raju Kushwaha
Veterinary University, Mathura
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Vinod Kumar
Veterinary University, Mathura
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Muneendra Kumar
Veterinary University, Mathura
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Shalini Vaswani
Veterinary University, Mathura
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Avinash Kumar
Veterinary University, Mathura
Keywords:
Exogenous fibrolytic enzyme, in vitro, paddy straw, Rumen fermentation, ureaAbstract
This study was conducted to evaluate the effect of urea and exogenous fibrolytic enzyme (EFE) treatment
on in-vitro digestibility and fermentation attributes of paddy straw. In this study, in vitro degradability
of nutrients in paddy straw as an effect of treatment of urea and EFE at various levels (1.0, 2.0, 3.0, 3.5, 4.0,
4.5, 5.0, 6.0, 7.0, 8.0 and 9.0 % of urea and 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0 and 9.0 g/kg DM of EFE) were
tested. For selecting the best levels of urea and EFE, test samples were incubated with strained rumen
liquor and different dosages levels were studied on rumen fermentation parameters like total gas production
along with in vitro DM and OM digestibility, ammonia nitrogen, pH and some calculated parameters
such as partitioning factor (PF), microbial biomass production (MBP) and efficiency of microbial protein
synthesis (EMPS) by incorporation of urea and EFE treated paddy straw. The result showed significant
improvement on in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD),
in vitro total gas production (IVTGP), MBP and PF at 4% and 8 g/kg DM of urea and EFE treatment level
in comparison to other levels. The optimum results were obtained at 4% and 8 g/kg DM of urea and EFE
treated paddy straw, hence a 4% and 8 g/kg DM dosage, respectively may be selected for in vivo study
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