Influence of Dietary Supplementation of Peppermint (Mentha piperita) and Lemongrass (Cymbopogon citratus) Essential Oils on Health Biomarkers in CrossbredCalves
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Authors
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Gautami Sarma
GBPUAT Pantnagar
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Jyoti Palod
Veterinary College , G.B.Pant University of Agriculture and Technology, Pantnagar
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Anita Arya
GBPUAT Pantnagar
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Shive Kumar
GBPUAT Pantnagar
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Sanjay Sharma
GBPUAT Pantnagar
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R. K. Sharma
GBPUAT Pantnagar
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S. K . Singh
GBPUAT Pantnagar
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Sumit Gangwar
GBPUAT Pantnagar
Keywords:
Antioxidant status, Crossbred calves, Essential oils, Hemato-biochemical parametersAbstract
The present study evaluated the effects of dietary supplementation with Mentha piperita essential oil (MPEO) and Cymbopogon citratus essential oil (CCEO) on the hematological profile, serum biochemical parameters, and blood antioxidant status in crossbred calves. 18 crossbred calves (Sahiwal × Holstein Friesian), aged between two weeks and three months, were allocated into three groups: T0 (control), T1 (MPEO at 0.2% of calf starter), and T2 (CCEO at 0.2% of calf starter). The study was conducted over 90 days, with blood samples collected at monthly intervals: 0, 30th, 60th and 90th day for study of haemato-biochemical parameters while at 45th and 90th day for antioxidant parameters . Hematological analysis showed a significant (P<0.05) increase in total erythrocyte count (TEC) and packed cell volume (PCV) in the T2 group, while eosinophil counts were significantly (P<0.05) reduced in both T1 and T2 groups of calves. No significant treatment effects were observed on total leukocyte count (TLC), differential leukocyte counts, or platelet counts. Biochemical analysis revealed significant reductions in serum urea (P<0.01) levels and alanine aminotransferase (P<0.05) in T1 and T2 group of calves. Lipid profile results revealed significant (P<0.05) reductions in triglycerides, total cholesterol (P<0.01), LDL, and VLDL in Cymbopogon citratus essential oil group and an increase in HDL cholesterol in both Mentha piperita essential oil (MPEO) and Cymbopogon citratus essential oil groups. As regards antioxidant parameters, lipid peroxidase (LPO) was significantly (P<0.05) reduced, and superoxide dismutase (SOD) activity was significantly (P<0.01) increased in the Cymbopogon citratus essential oil group of calves. The results suggest that Cymbopogon citratus essential oil supplementation positively influences hematological parameters, lipid metabolism, and antioxidant status, with potential benefits for calf health and performance. Further studies are recommended to explore the long-term effects of these essential oils.
References
Ademuyiwa, A. J. and Grace, O. K. 2015. The effects of Cymbopogon citratus (Lemon grass) on the antioxidant profiles wistar albino rats. Merit Research Journal of Environmental Science and Toxicology. 3(4): 51-58.
Akbari, M., Torki, M. and Kaviani, K. 2016. Single and combined effects of peppermint and thyme essential oils on productive performance, egg quality traits, and blood parameters of laying hens reared under cold stress condition (6.8±3 °C). International Journal of Biometeorology. 60: 447-454.
Al-Azzami, A. A. and Mohammed, T. T. 2023. Effect of adding dry leaves of lemongrass (Cymbopogon citratus) to the diet on some biochemical tests of blood in broiler (Ross 308). In IOP Conference Series: Earth and Environmental Science., December, 2023. (Vol.1252, No.1, p.012123). IOP Publishing.
Alagawany, M., El-Saadony, M. T., Elnesr, S. S., Farahat, M., Attia, G., Madkour, M. and Reda, F. M. 2021. Use of lemongrass essential oil as a feed additive in quail's nutrition: its effect on growth, carcass, blood biochemistry, antioxidant and immunological indices, digestive enzymes and intestinal microbiota. Poultry Science. 100(6): 101172.
Ali, A., Wu, H., Ponnampalam, E. N., Cottrell, J. J., Dunshea, F. R. and Suleria, H. A. 2021. Comprehensive profiling of most widely used spices for their phenolic compounds through LC-ESI-QTOF-MS2 and their antioxidant potential. Antioxidants. 10(5): 721-744.
Al-Janabi, A. A., Alsalami M. S. and Noaman A. I. 2023. The effect of lemongrass, Cymbopogon citratus, on some physiological parameters in male Awassi lambs. Iranian Journal of Ichthyology. 10: 47-54.
Al-Kassie, G. A. 2010. The role of peppermint (Mentha piperita) on performance in broiler diets. Agriculture and Biology Journal of North America. 1(5): 1009-1013.
Arab Ameri, S., Samadi, F., Dastar, B. and Zerehdaran, S. 2015. Effect of peppermint (Mentha piperita) powder on immune response of broiler chickens in heat stress. Iranian Journal of Applied Animal Science. 6(2): 435-445.
Bharti, R., Ahuja, G., Sujan, G. P. and Dakappa, S. S. 2013. A review on medicinal plants having antioxidant potential. Journal of Pharmacy Research. 5(8): 4278-4287.
Brahmi, F., Khodir, M., Mohamed, C. and Pierre, D. 2017. Aromatic and medicinal plants - back to nature, 2nd Edn. IntechOpen, Croatia. p47.
Chiofalo, V., Liotta, L., Fiumanò, R., Riolo, E. B. and Chiofalo, B. 2012. Influence of dietary supplementation of Rosmarinus officinalis L. on performances of dairy ewes organically managed. Small Ruminant Research. 104(1-3): 122-128.
Coelho, M. G., da Silva, A. P., de Toledo, A. F., Cezar, A. M., Tomaluski, C. R., Barboza, R. D. and Bittar, C. M. 2023. Essential oil blend supplementation in the milk replacer of dairy calves: Performance and health. PLoS One. 18(10): e0291038.
Coimbra, A., Miguel, S., Ribeiro, M., Coutinho, P., Silva, L., Duarte, A. P. and Ferreira, S. 2022. Thymus zygis essential oil: Phytochemical characterization, bioactivity evaluation and synergistic effect with antibiotics against Staphylococcus aureus. Antibiotics. 11:146-162.
Coles, E.H. 1986. Veterinary Clinical Pathology, 4th Edn. W.B. Saunders Co., Philadelphia. 43-70 р.
Donnelly, K. L., Smith, C. I., Schwarzenberg, S. J., Jessurun, J., Boldt, M. D. and Parks, E. J. 2005. Sources of fatty acids stored in liver and secreted via lipoproteins in patients with nonalcoholic fatty liver disease. The Journal of Clinical Investigation. 115(5): 1343-1351.
Dorantes-Iturbide, G., Orzuna-Orzuna, J. F., Lara-Bueno, A., Mendoza-Martínez, G. D., Miranda-Romero, L. A. and Lee-Rangel, H. A. 2022. Essential oils as a dietary additive for small ruminants: A meta-analysis on performance, rumen parameters, serum metabolites, and product quality. Veterinary Sciences. 9(9): 475-481.
El-Essawy, A. M., Anele, U. Y., Abdel-Wahed, A. M., Abdou, A. R. and Khattab, I. M. 2021. Effects of anise, clove, and thyme essential oils supplementation on rumen fermentation, blood metabolites, milk yield, and milk composition in lactating goats. Animal Feed Science and Technology. 271: 114760.
Farghaly, M. M. and Abdullah, M. A. M. 2021. Effect of dietary oregano, rosemary, and peppermint as feed additives on nutrients digestibility, rumen fermentation, and performance of fattening sheep. Egyptian Journal of Nutrition and Feeds. 24(3): 365–376.
Franz, C. H., Novak, J., Hajdari, A. and Mustafa, B. 2010. Total flavonoids, total phenolics and antioxidant activity of Betonica officinalis L. from Kosovo. In IV International Symposium on Breeding Research on Medicinal and Aromatic Plants-ISBMAP2009 860. 17, June, 2009. pp75-80.
Ghanima, M. M. A., Swelum, A. A., Shukry, M., Ibrahim, S. A., Abd El-Hack, M. E., Khafaga, A. F., Alhimaidi, A. R., Ammari, A. A., El-Tarabily, K. A. and Younis, M. E. M. 2021. Impacts of tea tree or lemongrass essential oils supplementation on growth, immunity, carcass traits, and blood biochemical parameters of broilers reared under different stocking densities. Poultry Science. 100(11): 101443.
Grundy, S. M. and Denke, M. A. 1990. Dietary influences on serum lipids and lipoproteins. Journal of Lipid Research. 31: 1149-1172.
Hosoda, K., Kuramoto, K., Eruden, B., Nishida, T. and Shioya, S. 2005b. The effects of three herbs as feed supplements on blood metabolites, hormones, antioxidant activity, IgG concentration, and ruminal fermentation in Holstein steers. Asian-Australasian Journal of Animal Sciences. 19(1): 35-41.
Ishfaq, A., Bhat, A. R., Ganai, A. M., Beigh, Y. A. and Sheikh, G. G. 2019. Effect of Pudina (Mentha piperita) supplementation on nutrient utilization and blood biochemical parameters of sheep. Indian Journal of Animal Nutrition. 36: 146-152.
Karageorgou, A., Tsafou, M., Goliomytis, M., Hager-Theodorides, A., Politi, K. and Simitzis, P. 2023. Effect of dietary supplementation with a mixture of natural antioxidants on milk yield, composition, oxidation stability, and udder health in dairy ewes. Antioxidants. 12(8): 1571-1583.
Khalifah, A. M., Abdalla, S. A., Dosoky, W. M., Shehata, M. G. and Khalifah, M. M. 2021. Utilization of lemongrass essential oil supplementation on growth performance, meat quality, blood traits and caecum microflora of growing quails. Annals of Agricultural Sciences. 66(2): 169-175.
Khamisabadi, H. and Fazaeli, H. 2021. Effect of Thymus vulgaris or peppermint on lactating Sanjabi ewe performance, milk composition, lamb growth, and relevant blood metabolites. Journal of Medicinal Plants and By-products. 10(Special): 95–101.
Khattab, M. S. A., El-Zaiat, H. M., Abd El Tawab, A. M., Matloup, O. H., Morsy, A. S., Abdou, M. M., Ebaid, H., Attia, M. F. A. and Sallam, S. M. A. 2017. Impact of lemongrass and galangal as feed additives on performance of lactating Barki goats. International Journal of Dairy Science. 12(3): 184–189.
Kris-Etherton, P. M. and Yu, S. 1997. Individual fatty acid effects on plasma lipids and lipoproteins: human studies. The American Journal of Clinical Nutrition. 65: 1628S-1644S.
Kumar, V. S., Inamdar, M. N. and Viswanatha, G. L. 2011. Protective effect of lemongrass oil against dexamethasone induced hyperlipidemia in rats: possible role of decreased lecithin cholesterol acetyl transferase activity. Asian Pacific Journal of Tropical Medicine. 4(8): 658-660.
Nehme, R., Andrés, S., Pereira, R. B., Ben Jemaa, M., Bouhallab, S., Ceciliani, F., López, S., Rahali, F. Z., Ksouri, R., Pereira, D. M. and Abdennebi-Najar, L. 2021. Essential oils in livestock: from health to food quality. Antioxidants. 10: 330-366.
Ojo, O., Kabutu, F., Bello, M. and Babayo, U. 2006. Inhibition of paracetamol-induced oxidative stress in rats by extracts of lemongrass (Cymbopogon citratus) and green tea (Camellia sinensis) in rats. African Journal of Biotechnology. 5(12): 1227-1232.
Orzuna-Orzuna, J. F., Dorantes-Iturbide, G., Lara-Bueno, A., Miranda-Romero, L. A., Mendoza-Martínez, G. D. and Santiago-Figueroa, I. 2022. A meta-analysis of essential oils use for beef cattle feed: rumen fermentation, blood metabolites, meat quality, performance and, environmental and economic impact. Fermentation. 8(6): 254.
Palhares Campolina, J., Gesteira Coelho, S., Belli, A. L., Samarini Machado, F. R., Pereira, L. G. R., Tomich, T. A., Carvalho, W. S., Silva, R. O. L., Voorsluys, A. V., Jacob, D. and Magalhães Campos, M. 2021. Effects of a blend of essential oils in milk replacer on performance, rumen fermentation, blood parameters, and health scores of dairy heifers. PLoS One. 16(3): e0231068.
Patra, A. K., Park, T., Braun, H. S., Geiger, S., Pieper, R., Yu, Z. and Aschenbach, J. R. 2019. Dietary bioactive lipid compounds rich in menthol alter interactions among members of ruminal microbiota in sheep. Frontiers in Microbiology. 10: 2038.
Puvadolpirod, S. and Thaxton, J. P. 2000. Model of physiological stress in chickens 2. Dosimetry of adrenocorticotropin. Poultry Science. 79: 370-376.
Rahman, A., Bayram, I. and Gultepe, E. E. 2021. Effect of Mentha on performance, haematological and biochemical parameters in laying hens. South African Journal of Animal Science. 51(2): 221-230.
Rahman, M. A., Redoy, M. R. A., Chowdhury, R. and Al Mamun, M. 2024. Effect of dietary supplementation of plantain herb, lemongrass and their combination on milk yield, immunity, liver enzymes, serum, and milk mineral status in dairy cows. Journal of Advanced Veterinary and Animal Research. 11(1): 185-193.
Rekiel, A., Beyga, K. and Vaško, V. 2007. Effect of backfat in point P2 and of body weight of primiparous sows in high pregnancy on their condition at weaning. Rocz Nauk Pol Tow Zoot. 3: 89-104.
Snedecor, G. W. and Cochran, W. B. 1994. Statistical Methods. 8th Edn., Iowa State University Press, Iowa. USA. p491.
Srivastava, S. 2021. Impact of dietary supplementation of lemongrass and peppermint essential oils on the performance of Japanese quail. Doctoral Thesis, G. B. Pant University of Agriculture and Technology, Uttarakhand, India.
Su, G., Zhou, X., Wang, Y., Chen, D., Chen, G., Li, Y. and He, J. 2018. Effects of plant essential oil supplementation on growth performance, immune function and antioxidant activities in weaned pigs. Lipids in Health and Disease. 17: 1-10.
Uchida, N. S., Silva-Filho, S. E., Aguiar, R. P., Wiirzler, L. A. M., Cardia, G. F. E. and Cavalcante, H. A. O. 2017. Protective effect of Cymbopogon citratus essential oil in experimental model of acetaminophen-induced liver injury. The American Journal of Chinese Medicine. 45(3): 515–532.
Witkowska, D., Sowińska, J., Murawska, D., Matusevičius, P., Kwiatkowska-Stenzel, A., Mituniewicz, T. and Wójcik, A. 2019. Effect of peppermint and thyme essential oil mist on performance and physiological parameters in broiler chickens. South African Journal of Animal Science. 49(1): 29-39.
Zeeshan, M., Masood, S., Ashraf, S., Bokhar, S. G., Zainab, H., Ijaz, S. and Usman, M. M. 2023. Efficacy of mannan-oligosaccharide and live yeast feed additives on performance, rumen morphology, serum biochemical parameters, and muscle morphometric characteristics in buffalo calves. arXiv preprint. arXiv:2308. 07456.
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