Effects of Bacterial Inoculants and Xylanase on Silage Fermentation Characteristics of Wheat straw and Pasture hay based Green Maize Silage
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Authors
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Saman Yusufkhan Belim
PhD Scholar,Department of Animal Nutrition,College of Veterinary Science and Animal Husbandry,Kamdhenu University, Junagadh
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Harish H. Savsani
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Yash G. Kansagara
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Mitesh R. Chavda
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Mulraj D. Odedara
Keywords:
Lactobacillus fermentum, Lactobacillus plantarum, Pasture hay, Silage,Wheat strawAbstract
Different silages were prepared using green maize fodder and wheat straw and pasture hay separately in the proportion of 10:0 & 7:3 ratio in plastic jar of 3 kg capacity by adding common salt @ 0.5%, urea @ 1% and molasses @ 1.5% in each silage with nine different treatments, viz., Control (only green maize), WS (green maize and wheat straw in 7:3 ratio), X (WS added with Xylanase), LF (WS added with Lactobacillus fermentum), LPLF (WS added with both bacterial inoculants), PH (green maize and pasture hay in 7:3 ratio), XPH (PH added with Xylanase), LFPH (PH added with Lactobacillus fermentum), LPLFPH (PH added with both bacterial inoculants). Xylanase, L. plantarum and L. fermentum were used @ 1500 IU/g, 1 x 106 cfu/g and 2 x 106 cfu/g, respectively. All silages were evaluated in terms of their proximate composition, cell wall constituents and quality parameters of silage on 45 days of ensiling. Bacterial inoculants significantly reduce DM content of silage. LPLF silage and XPH and LFPH significantly increases crude protein content. X and LPLFPH shows significant improvement in EE content. LF shows significantly improve values of CF and NFE. X and XPH silage significantly lowers NDF and ADF content. LPLF and XPH silage significantly improves cellulose while additives do not have any significant effect on hemicellulose content. Bacterial inoculants and Xylanase significantly improves TVFA, WSC and LA to lower silage pH for very good silage. Xylanase, Lactobacillus fermentum and combination of bacterial inoculants with wheat straw and pasture hay silage, respectively significantly reduces ammonia nitrogen for excellent silage quality. Thus, it is concluded that Xylanase and/or Latobacillus fermentum significantly improves overall silage fermentation characteristics and nutrient content in green maize based silage along with wheat straw or pasture hay in 7:3 ratio.
References
Agarussi, M. C. N., Pereira, O. G., De Paula, R. A., Da Silva, V. P., Roseira, J. P. S. and e Silva, F. F. (2019). Novel lactic acid bacteria strains as inoculants on alfalfa silage fermentation. Scientific Reports. 9(1): 1-9.
AOAC (2023). Official Methods of Analysis. 22nd Edition, Association of official analytical collaboration international (AOAC), Washington, (DC), USA.
Carvalho, B. F., Sales, G. F. C., Schwan, R. F. and Avila, C. L. S. (2020). Criteria for lactic acid bacteria screening to enhance silage quality. Journal of Applied Microbiology. 130(2): 341-355.
Chen, L., Li, J., Dong, Z. and Shao, T. (2019). Effects of lactic acid bacteria inoculants and fibrolytic enzymes on the fermentation quality, in vitro degradability, ruminal variables and microbial communities of high‐moisture alfalfa silage. Grassland Science. 65(4): 216-225.
Dakore, S. (2018). Effect of bacterial inoculants and exogenous enzymes on the ensiling characteristic of sugargraze silage and nutrient utilization in crossbred calves. M.V.Sc. Thesis, ICAR-National Dairy Reserch Institute, Karnal, Haryana, India.
Filya, I. (2003). The effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corn and sorghum silages. Journal of Dairy Science. 86(11): 3575–3581.
Govea, F. E., Muck, R. E., Broderick, G. A. and Weimer, P. J. (2013). Lactobacillus plantarum effects on silage fermentation and in vitro microbial yield. Animal Feed Science and Technology. 179(1-4): 61-68.
Guo, G., Yuan, X., Li, L., Wen, A. and Shao, T. (2014). Effects of fibrolytic enzymes, molasses and lactic acid bacteria on fermentation quality of mixed silage of corn and hulless–barely straw in the Tibetan Plateau. Grassland Science. 60(4): 240-
Jalc, D., Laukova, A. and Kisidayova, S. (2010). Effect of inoculants on fermentation parameters and chemical composition in grass and corn silages. Slovak Journal of Animal Science. 43(3): 141-146.
Jalc, D., Laukova, A., Simonova, M., Varadyova, Z. and Homolka, P. (2009). The use of bacterial inoculants for grass silage: their effects on nutrient composition and fermentation parameters in grass silages. Czech Journal of Animal Science. 54(2): 84-91.
Khota, W., Pholsen, S., Higgs, D. and Cai, Y. (2017). Fermentation quality and in vitro methane production of sorghum silage prepared with cellulase and lactic acid bacteria. Asian-Australian Journal of Animal Sciences. 30(11): 1568-1574.
Kung Jr., L. (2010) A Review on Silage Additives and Enzymes. Department of Animal and Food Sciences University of Delaware Newark, DE 19717-1303.
Marbun, T. D., Lee, K., Song, J., Kwon, C. H., Yoon, D., Lee, S. M., Kang, J. Lee, C., Cho, S. and Kim, E. J. (2020). Effect of lactic acid bacteria on the nutritive value and in vitro ruminal digestibility of maize and rice straw silage. Applied Sciences. 10(21): 7801.
Nkosi, B. D., Vadlani, P. V., Brijwani, K., Nanjunda, A. and Meeske, R. (2012). Effects of bacterial inoculants and an enzyme on the fermentation quality and aerobic stability of ensiled whole-crop sweet sorghum. South African Journal of Animal Science. 42(3): 232-240.
Prasad, J. (2015). Chemical analysis of silage. In: Principles and practices of animal nutrition. 4th revised edition. Kalyani publishers. Ludhiana.
Snedecore, G. and Cochran, W. (1994). Statistical Methods. 8th edition. Oxford and IBH. New Delhi. Pp: 503.
Su, R., Ni, K., Wang, T., Yang, X., Zhang, J., Liu, Y., Shi, W., Yan, L., Jie, C. and Zhong, J. (2019). Effects of ferulic acid esterase-producing Lactobacillus fermentum and cellulase additives on the fermentation quality and microbial community of alfalfa silage. PeerJ. 7: e7712.
Van Soest, P. J., Robertson, J. B. and Lewis, B. A. (1991). Methods for dietary fiber neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science. 74(10): 3583-3597.
Xing, L., Chen, L. J. and Han, L. J. (2009). The effect of an inoculant and enzymes on fermentation and nutritive value of sorghum straw silages. Bioresource Technology. 100(1): 488-491.
Yadav, S. K. (2018). The effect of Lactic acid bacterial inoculants and exogenous enzymes on maize silage quality and nutrient utilisation in buffalo calves. M.V.Sc. Thesis, ICAR-National Dairy Reserch Institute, Karnal, Haryana, India.
Yemm, E. W. and Willis, A. (1954). The estimation of carbohydrates in plant extracts by anthrone. Biochemical Journal. 57(3): 508-514.
Zhao, C., Wang, L., Ma, G., Jiang, X., Yang, J., Lv, J. and Zhang, Y. (2021). Cellulase interact with lactic acid bacteria to affect fermentation quality, microbial community and ruminal degradability in mixed silage of soyabean residue and corn stover. Animals. 11(2): 334.
Zielińska, K. J. and Fabiszewska, A. U. (2017). Improvement of the quality of maize grain silage by a synergistic action of selected lactobacilli strains. World Journal of Microbiology and Biotechnology. 34(9): 1-8.
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