Rapid Estimation of Chlorpyrifos in Feed Samples using Gas Chromatograph- Micro Electron Capture Detector (GC-µECD)
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Authors
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S. B. N. Rao
National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore
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K S Prasad
Retired
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Athira Thomas
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Jenita M Tellis
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M. A. Pavan Kumar
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Naveen B Devaraju
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C. C. Chethankumari
Keywords:
Chloropyrifos , Gas Chromatograph-Electron Capture Detector, Feeds, QuEChERSAbstract
In this research paper, we have standardized a rapid method for estimation of chlorpyrifos residues in feed samples using Gas Chromatograph- Micro Electron Capture Detector(GC-µECD). The extraction method used for this study was QuEChERS viz. quick, easy, cheap, effective, rugged and safe. Validation was done by performing the following parameters; Linearity, Limit of detection (LOD), Limit of quantification (LOQ), Matrix effect and Recovery percentage. A regression equation with regression coefficient (r2) of 0.9913 was obtained indicating excellent linearity. LOD and LOQ of the method were obtained as 0.1 mg/L and 0.4 mg/L respectively. Recovery percentage of the spiked concentration 0.1 ppm was obtained as 85.7% and for 0.5 ppm it was 77.7%. The method qualified the required parameters for analysis of chloropyriphos in the different classes of feeds.
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