Identification and in-silico characterization of Serpin genes in legumes genomes

PAWAN S MAINKAR; MANOJ M L; DEEPANSHU JAYASWAL; YAMINI AGARWAL; RAKESH K PRAJAPAT; REKHA KANSAL

doi:10.56093/ijas.v90i9.106624

Authors

PAWAN S MAINKAR ICAR-National Institute for Plant Biotechnology, Lal Bahadur Shastri Building, Pusa Campus, New Delhi 110 012
MANOJ M L ICAR-National Institute for Plant Biotechnology, Lal Bahadur Shastri Building, Pusa Campus,New Delhi 110 012
DEEPANSHU JAYASWAL ICAR-National Institute for Plant Biotechnology, Lal Bahadur Shastri Building, Pusa Campus,New Delhi 110 012
YAMINI AGARWAL ICAR-National Institute for Plant Biotechnology, Lal Bahadur Shastri Building, Pusa Campus,New Delhi 110 012
RAKESH K PRAJAPAT ICAR-National Institute for Plant Biotechnology, Lal Bahadur Shastri Building, Pusa Campus,New Delhi 110 012
REKHA KANSAL ICAR-National Institute for Plant Biotechnology, Lal Bahadur Shastri Building, Pusa Campus,New Delhi 110 012

https://doi.org/10.56093/ijas.v90i9.106624

Keywords:

Legumes, Proteases, Reactive center loop, Serine protease inhibitor, Serpins

Abstract

Serine protease inhibitors (serpins) are a unique family of protease inhibitor containing mobile reactive center loop. The availability of genome sequences of pigeonpea, soybean, commanbean, cowpea, mungbean and adzuki bean provided an opportunity to search for the serpin genes. A total of seven serpin genes were identified in the above legume genomes. Phylogenetic analysis of legume serpins, devided them into two major clades (Clade-I:VanSerpin, VraSerpin, VunSerpin, PvuSerpin and clade-II: GmaSerpin1, GmaSerpin2 and CcaSerpin) based on conserved reactive center (P2-P1’), domains and exon-intron boundary in the gene structure. The common reactive center of all the seven serpins indicated their ability to target cognate proteases and play an important role in response to insects, fungal pathogens and also the drought.

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