Efficacy of phosphate solubilizing microorganisms in utilizing native phosphorus in an alkaline alluvial soil of North India

KHURSHID ALAM; MANDIRA BARMAN; SIBA PRASAD DATTA; SARVENDRA KUMAR; K ANNAPURNA; LIVLEEN SHUKLA; DEBASHIS CHAKRABORTY

doi:10.56093/ijas.v90i11.108594

Authors

KHURSHID ALAM Ph D Scholar, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
MANDIRA BARMAN Corresponding author and Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
SIBA PRASAD DATTA Professor and Principal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
SARVENDRA KUMAR Scientist, Division of Soil Science and Agricultural Chemistry, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
K ANNAPURNA Head and Principal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
LIVLEEN SHUKLA Principal Scientist, Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
DEBASHIS CHAKRABORTY National Fellow, Division of Agricultural Physics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India

https://doi.org/10.56093/ijas.v90i11.108594

Keywords:

Alluvial soil, Fixed pool, Phosphate solubilizing microorganism (PSM), P release pattern, Solubilization

Abstract

Utilization of phosphate solubilizing microorganisms (PSMs) to solubilize the fixed P pool in soil is a promising method considering the shrinking availability of raw material (rock phosphate) of phosphatic fertilizer production in one hand and global food security on the other. An incubation study was carried out for 90 days to assess the ability of the two PSMs, namely Enterobacter sp. and Aspergillus niger in mediating the release of P from fixed pool in an alluvial soil (pH= 8.30) of IARI farm, New Delhi. On an average, both the microorganisms significantly increased the release of P into solution from fixed P pool of soil. However, Enterobacter sp. treated soil showed better P release (0.52 mg/kg) than that of Aspergillus niger treated soil (0.44 mg/kg) over uninoculated control (0.42 mg/kg). Enterobacter sp. was capable in mediating P release into soil solution during the whole period of incubation. Soil treated with Enterobacter sp. showed almost similar level of solution P concentration from 2nd week to 6th week of incubation and after that, there was a decrease. Whereas, A. niger was able to mediate P release into soil solution only up to 28 days of incubation, after that solution P concentration of treated soil became statistically at par with untreated control. Thus, Enterobacter sp. performed better than A. niger in utilizing native P in alluvial soil.

Downloads

Download data is not yet available.

References

Balemi T and Negisho K. 2012. Management of soil phosphorus and plant adaptation mechanisms to phosphorus stress for sustainable crop production: a review. Journal of Soil Science and Plant Nutrition 12(3): 547-562. DOI: https://doi.org/10.4067/S0718-95162012005000015

Bouyoucos G J. 1962. Hydrometer method improved for making particle size analyses of soils. Agronomy Journal 54(5): 464- 465. DOI: https://doi.org/10.2134/agronj1962.00021962005400050028x

Hanway J J and Heidel H. 1952. Soil analysis methods as used in the Iowa State College Soil Testing Laboratory. Iowa Agriculture 57: 1-13.

Illmer P and Schinner F. 1992. Solubilization of inorganic phosphates by microorganisms isolated from forest soils. Soil Biology and Biochemistry 24(4): 389-395. DOI: https://doi.org/10.1016/0038-0717(92)90199-8

Illmer P, Barbato A and Schinner F. 1995. Solubilization of hardly-soluble AlPO4 with P-solubilizing microorganisms. Soil Biology and Biochemistry 27(3): 265-270. DOI: https://doi.org/10.1016/0038-0717(94)00205-F

Jackson M L. 1973. Methods of Chemical Analysis. Prentic Hall., EngleWood Cliffs, NTJ.

Khan M S, Zaidi A and Wani P A. 2007. Role of phosphate-solubilizing microorganisms in sustainable agriculture-a review. Agronomy for Sustainable Development 27(1): 29-43. DOI: https://doi.org/10.1051/agro:2006011

Khan M S, Zaidi A, Ahemad M, Oves M and Wani P A. 2010. Plant growth promotion by phosphate solubilizing fungi-current perspective. Archives of Agronomy and Soil Science 56(1): 73-98. DOI: https://doi.org/10.1080/03650340902806469

Mohammadi K. 2012. Phosphorus solubilizing bacteria: occurrence, mechanisms and their role in crop production. Resources and Environment 2(1): 80-85.

Nahas E. 1996. Factors determining rock phosphate solubilization by microorganisms isolated from soil. World Journal of Microbiology and Biotechnology 12(6): 567-572. DOI: https://doi.org/10.1007/BF00327716

Narsian V, Thakkar J and Patel H H. 1995. Mineral phosphate solubilization by Aspergillus aculeatus. Indian Journal of Experimental Biology 33: 91-93.

Owen D, Williams A P, Griffith G W and Withers P J. 2015. Use of commercial bio-inoculants to increase agricultural production through improved phosphrous acquisition. Applied Soil Ecology 86: 41-54. DOI: https://doi.org/10.1016/j.apsoil.2014.09.012

Richards L A. 1954. Diagnosis and Improvement of Saline and Alkaline soils. United States Department of Agriculture Handbook No. 60, United States Government Printing Office, Washington DC.

Roberts T L and Johnston A E. 2015. Phosphorus use efficiency and management in agriculture. Resources, Conservation and Recycling 105: 275-281. DOI: https://doi.org/10.1016/j.resconrec.2015.09.013

Singh H and Reddy M S. 2011. Effect of inoculation with phosphate solubilizing fungus on growth and nutrient uptake of wheat and maize plants fertilized with rock phosphate in alkaline soils. European Journal of Soil Biology 47(1): 30-34. DOI: https://doi.org/10.1016/j.ejsobi.2010.10.005

Snedecor G W and Cochran W G. 1967. Statistical Methods, 6th edition, p 31. Ames. Iowa, USA: Iowa State University Press.

Subbaiah B V and Asija G L. 1956. A rapid procedure for estimation of available nitrogen in soil. Current Science 25: 259-260.

Waksman S A and Fred E B. 1922. A tentative outline of the plate method for determining the number of micro-organisms in the soil. Soil Science 14(1): 27-28. DOI: https://doi.org/10.1097/00010694-192207000-00004

Walkley A and Black I A. 1934. An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Science 37(1): 29-38. DOI: https://doi.org/10.1097/00010694-193401000-00003

Wang T, Liu M Q and Li H X. 2014. Inoculation of phosphate-solubilizing bacteria Bacillus thuringiensis B1 increases available phosphorus and growth of peanut in acidic soil. Acta Agriculturae Scandinavica, Section B-Soil & Plant Science 64(3): 252-259. DOI: https://doi.org/10.1080/09064710.2014.905624

Watanabe F S and Olsen S R. 1965. Test of an ascorbic acid method for determining phosphorus in water and NaHCO3 extracts from soil. Soil Science Society of America Journal 29(6): 677-678. DOI: https://doi.org/10.2136/sssaj1965.03615995002900060025x