Biochemical responses to natural and accelerated ageing on seed quality in Indian mustard (Brassica juncea)

PRADEEP SINGH; V S MOR; ANURAG MALIK; HIMANI PUNIA; AXAY BHUKER

doi:10.56093/ijas.v92i7.113603

Authors

PRADEEP SINGH CCS Haryana Agricultural University, Hisar, Haryana 125 004, India
V S MOR CCS Haryana Agricultural University, Hisar, Haryana 125 004, India
ANURAG MALIK CCS Haryana Agricultural University, Hisar, Haryana 125 004, India https://orcid.org/0000-0002-5037-425X
HIMANI PUNIA CCS Haryana Agricultural University, Hisar, Haryana 125 004, India
AXAY BHUKER CCS Haryana Agricultural University, Hisar, Haryana 125 004, India

https://doi.org/10.56093/ijas.v92i7.113603

Keywords:

accelerated aging, mustard, quality, superoxide dismutase, viability, vigor

Abstract

Indian mustard [Brassica juncea (L.) Czern. & Coss.] is an important oilseed crop of many tropical and subtropical
countries of the world. The present study was carried out at research farm of Department of Seed Science
and Technology, CCS Haryana Agricultural University, Hisar during winter (rabi) season 2017–18 and 2018–19.
The objective of present study was to evaluate the effect of natural and accelerated aging on biochemical constitutes in 3 Indian mustard varieties, viz. RH-30, RH-9304 and RH-0406 with three seed lots (fresh seed, 1-year old seed, 2-year-old seed). Naturally aged seed lots of variety RH-30 was found to meet out Indian Minimum Seed Certification Standard after 1-year under ambient condition. Tetrazolium test indicated that the viability of Indian mustard seeds decreased after increasing the ageing duration. Maximum seed viability recorded in the variety RH-30 and minimum was recorded in variety RH-0406. Activities of all the enzymes, viz. superoxide dismutase, dehydrogenase and peroxidase significantly decreased with natural ageing; RH-30 was found to be a good storer variety. The maximum performance for standard germination and enzymes activity was observed in fresh seed lots. Results showed that among different Indian mustard varieties, RH-30 and RH-9304 were found best responsive with minimal seed deterioration effects, and better germination and seedling establishment potential.

Downloads

Download data is not yet available.

Author Biographies

PRADEEP SINGH, CCS Haryana Agricultural University, Hisar, Haryana 125 004, India

Department of seed science & technology
V S MOR, CCS Haryana Agricultural University, Hisar, Haryana 125 004, India

Seed Science and technology
ANURAG MALIK, CCS Haryana Agricultural University, Hisar, Haryana 125 004, India

Department of Seed and Technology
HIMANI PUNIA, CCS Haryana Agricultural University, Hisar, Haryana 125 004, India

Department of Biochemistry
AXAY BHUKER, CCS Haryana Agricultural University, Hisar, Haryana 125 004, India

Seed Science and technology

References

Balesevic-Tubic S, Malencic D, Tatic M and Miladinovic J. 2007. Influence of aging process on biochemical changes in sunflower seed. Helia 28(42): 107–14. DOI: https://doi.org/10.2298/HEL0542107B

Bhanuprakash K, Yogeesha H S and Arun M N. 2010. Physiological and biochemical changes in relation to seed quality in ageing bell pepper seed. Indian Journal of Agricultural Sciences 80(9): 777–80.

Chauhan D S, Deswal D P, Dahiya O S and Punia R C. 2011. Change in storage enzymes activities in natural and accelerated aged seed of wheat (Triticum aestivum). Indian Journal of Agricultural Sciences 81(11): 1037–40.

Demirkaya M and Sivritepe H O. 2011. Physiological and biochemical changes occur in onion seeds during ageing. Tarim Bilimleri Dergisi 17(2): 105–12.

Ghassemi-Golezani K, Bakhshy J, Raey R and Hossainzadeh-Mahootchy A. 2010. Seed vigour and field performance of winter oilseed rape (Brassica napus L.) cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 38(3):146–150.

Giannopolitis C N and Reis S K. 1977. Superoxide dismutase occurrence in higher plants. Plant Physiology 59: 309–14. DOI: https://doi.org/10.1104/pp.59.2.309

Hussein J, Hussein S, Abdulla I and Yasser O M. 2012. Effect of accelerated ageing on vigour of local maize seeds in term of electrical conductivity and relative growth rate. Iraqi Journal of Science 53(2): 285–91.

ISTA. 1999. International rules for seed testing. Seed Science & Technolnology (Suppl) 27: 1–334.

Kanwar R, Mehta D K and Manohar Lal. 2014. Effect of seed priming on physiological parameters of aged and non-aged seeds of bitter gourd. International journal of Farm Sciences 4(3): 24–32.

Kapoor N, Arya A, Siddiqui M A, Amir A and Kumar H. 2010. Seed deterioration in chickpea (Cicer arietnum L) under accelerated ageing Asian Journal of Plant Science 9(3): 158–62. DOI: https://doi.org/10.3923/ajps.2010.158.162

Kavitha S, Meneka C and Ananthi M. 2017. Deterioration in sesame seeds under natural and accelerated ageing. International Journal of Chemical Studies 5(4): 1141–46.

Kittock D L and Law A G. 1968. Relationship of seedling vigour to respiration and tetrazolium chloride reduction by germinating wheat seeds. Agronomy Journal 60: 286–88. DOI: https://doi.org/10.2134/agronj1968.00021962006000030012x

Kumar V, Malik T P, Tehlan S K and Kumar A. 2017. Evaluation of seed quality in naturally aged seed lots of coriander. International journal of Plant and Soil Science 15(6): 1–8. DOI: https://doi.org/10.9734/IJPSS/2017/32722

Kumar S, Radhamani J and Srinivasan K. 2011. Physiological and biochemical changes in seeds of karanj (Pongamiapinnata) under different storage conditions. Indian Journal of Agricultural Sciences 81(5): 423–28.

Lather B P, Deshwal S and Punia R C. 2010. Vigur determines performance of cotton seed in fields. (In) Proceeding of National Symposium on Food Security in Context of Changing Climate. The Society of Agriculture Professionals. Kanpur Oct.-Nov. 01 Abstract 5: 14(93).

Loycrajjou L, Steven Y, Groot P C, Belghazi M, Job C and Job D. 2008. Proteome wide characterization of seed ageing in Arabidopsis comparison between artificial and natural ageing. Plat Physiology 148: 620–41. DOI: https://doi.org/10.1104/pp.108.123141

Moore R P. 1973. Tetrazolium stain for assessing seed quality. Seed Ecology, pp 347–66. W Heydecker (Eds). The Pennsylvania State University Park, PA.

Punia H, Madan S, Malik A and Sethi S K. 2019. Stability analysis for quality attributes in durum wheat (Triticum durum L.) genotypes. Bangladesh Journal of Botany 48: 967–72. DOI: https://doi.org/10.3329/bjb.v48i4.49036

Punia Tokas J, Malik A, Sangwan S, Rani A, Yashveer S, Alansi S, Hashim M J and El-Sheikh M A. 2021a. Genome-wide transcriptome profiling characterization and functional identification of NAC transcription factors in sorghum under salt stress. Antioxidants 10(10): 1605. DOI: https://doi.org/10.3390/antiox10101605

Punia H, Tokas J, Malik A, Bajguz A, El-Sheikh M A and Ahmad P. 2021b. Ascorbate–glutathione oxidant scavengers metabolome analysis and adaptation mechanisms of ion exclusion in sorghum under salt stress. International Journal of Molecular Sciences 22: 13249. DOI: https://doi.org/10.3390/ijms222413249

Punia H, Tokas J, Mor V S, Bhuker A, Malik A, Singh N, Satpal Alsahli A A and Hefft D I. 2021c. Deciphering reserve mobilization antioxidant potential and expression analysis of starch synthesis in sorghum seedlings under salt stress. Plants 10(11): 2463. DOI: https://doi.org/10.3390/plants10112463

Rai H, Ovais H P, Dahiya O S and Jakhar S S. 2017. Seed vigour assessment in different varieties of Indian mustard (Brassica juncea (L.) Czern & Coss). International Journal of Current Microbiology and Applied Sciences 6(10): 1930–36. DOI: https://doi.org/10.20546/ijcmas.2017.610.232

Shannon L M, Kay E and Law J Y. 1966. Peroxidase isoenzyme from horse radish roots: Isolation and physical properties. Journal of Biological Chemistry 241: 166–2172. DOI: https://doi.org/10.1016/S0021-9258(18)96680-9

Singh B, Bhuker A, Mor V S, Dahiya O S and Punia R C. 2015. Effect of natural ageing on seed quality of fenugreek (Trigonella foenumgraecum L.). International Journal of Scientific Research in Science and Technology 1(4): 243–48.

Tian X, Song S and Lei Y. 2008. Cell death and reactive oxigen species metabolism during accelerated ageing of soybean axes. Russian Journal of Plant Physiology 55(1): 33–40. DOI: https://doi.org/10.1134/S1021443708010032