Irradiation effect on leaf sclerophylly, gas exchange and stomata in sweet orange (Citrus sinensis)
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Keywords:
Leaf sclerophylly, Leaf gas exchange, Mutant, Stomata, Sweet orangeAbstract
The study was carried out in mutated population of sweet orange [Citrus sinensis (L.) Osbeck] cv. Mosambi developed from varying dose of gamma rays (10-35 Gy) to determine its effect on leaf sclerophylly, photosynthesis and leaf anatomical characters at the Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi during 2017 and 2018. As compared to wild type leaf area, fresh and dry mass was stimulated by 19.53, 18.42 and 20.37% in mutant GS-3 developed from 10 Gy, while in the mutants GS-33 and GS-35 developed from 35 Gy >50% inhibitory effects were noticed. Leaves were however, 82.06% more succulent in the mutant developed from 30 Gy. Mutated population showed dose dependent effect with regard to SLA, SLW and DFT. A significant decline in the photosynthetic rate and stomatal conductance was noticed at 35 Gy in GS-32, whereas mutant GS-34 developed from the same irradiation dose had the minimum transpiration rate. Stomata length and width in the mutants GS-17 and GS-15 as compared to wild type registered an increase of 25.50% and 15.96%. A general reduction in stomata number was observed in the mutated populations. Our result indicated alteration in traits of the mutated population which are of significance and can be used as a selection criterion for improvement of sweet orange.Downloads
References
Ahloowalia B S and Maluszynski M. 2001. Induced mutations–A new paradigm in plant breeding. Euphytica 118: 167–73. DOI: https://doi.org/10.1023/A:1004162323428
Anonymous. 2018. Indian Horticuiture Database, National Horticulture Board, 1st Advance Estimate. Ministry of Agriculture, Government of India.
Broertjes C and Van Harten A M. 2013. Applied Mutation Breeding for Vegetatively Propagated Vrops, Vol. 12. Elsevier, Amsterdam, Netherlands.
De Micco V, Arena C, Pignalosa D and Durante M. 2011. Effects of sparsely and densely ionizing radiation on plants. Radiation and Environmental Biophysics 50(1): 1–19. DOI: https://doi.org/10.1007/s00411-010-0343-8
Ennajeh M, Vadel A M, Cochard H and Khemira H. 2010. Comparative impacts of water stress on the leaf anatomy of a drought resistant and a drought sensitive olive cultivar. Journal of Horticultural Science and Biotechnology 85 (4): 289–94. DOI: https://doi.org/10.1080/14620316.2010.11512670
Hearne C J. 1984. Development of seedless orange and grapefruit cultivars through seed irradiation. Journal of the American Society for Horticultural Science 109: 270–73. DOI: https://doi.org/10.21273/JASHS.109.2.270
Kok D. 2011. Involvement of peroxidase activity in various sensitivity to gamma irradiation in scions of Cabernet Sauvignon and Merlot cvs (Vitis vinifera L.). Journal of Food, Agriculture and Environment 9(2): 392–96.
Mallick M, Awasthi O P, Paul V, Verma M K and Jha G. 2016. Effect of physical and chemical mutagens on leaf sclerophylly and stomatal characteristics of Kinnow mandarin mutants. Indian Journal of Horticulture 73(2): 291–93. DOI: https://doi.org/10.5958/0974-0112.2016.00063.3
Moghaddam S S, Jaafar H B, Aziz M A, Ibrahim R, Rahmat A B and Philip E. 2011. Flavonoid and leaf gas exchange responses of Centella asiatica to acute gamma irradiation and carbon dioxide enrichment under controlled environment conditions. Molecules 16(11): 8930–44. DOI: https://doi.org/10.3390/molecules16118930
Nobel P S. 1999. Physicochemical and Environmental Plant Physiology, 2nd edn. Academic Press, San Diego, California.
Qosim W A, Purwanto R, Wattimena G A and Witjaksono. 2011. Alteration of leaf anatomy of mangosteen (Garcinia mangostana L.) regenerants in vitro by gamma irradiation. Plant Mutation Reports 2(3): 15–16.
Sampson J. 1961. A method of replicating dry or moist surfaces for examination by light microscopy. Nature 191: 932. DOI: https://doi.org/10.1038/191932a0
Sharma L K, Kaushal M, Gill M I S and Bali S K. 2013. Germination and survival of Citrus Jambhiri seeds and epicotyls after treating with different mutagens under in vitro conditions. Middle-East Journal of Scientific Research 16(2): 250–55.
Widiastuti A, Sobir S and Suhartanto M R. 2010. Diversity analysis of mangosteen (Garcinia mangostana) irradiated by gamma-ray based on morphological and anatomical characteristics. Nusantara Bioscience 2: (1). DOI: https://doi.org/10.13057/nusbiosci/n020104
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