Evaluation of various substrates for mycorrhizal inoculum production

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  • MANOJ PARIHAR Banaras Hindu University, Varanasi, Uttar Pradesh 221 005, India
  • AMITAVA RAKSHIT Banaras Hindu University, Varanasi, Uttar Pradesh 221 005, India
  • KIRAN RANA Banaras Hindu University, Varanasi, Uttar Pradesh 221 005, India
  • GOPAL TIWARI Banaras Hindu University, Varanasi, Uttar Pradesh 221 005, India
  • SURENDRA SINGH JATAV Banaras Hindu University, Varanasi, Uttar Pradesh 221 005, India
  • HANUMAN RAM Banaras Hindu University, Varanasi, Uttar Pradesh 221 005, India



Arbuscular mycorrhizal fungi, Micronutrients, Porosity, Root colonization, WHC


In the present study, six media were standardized to investigate their suitability for on-farm arbuscular mycorrhizal fungi (AMF) production by examining their physical condition and nutrient status which could be a major determinant of AMF propagule in the final product. The experiment was conducted with Sorghum as a host plant which was inoculated with Funneliformis mosseae and Rhizoglomus intraradices. Solirite alone recorded 4.66–24.66% higher root colonization than other medium while for spore density in per g of finished product, solirite and red soil mixture (106.33) was found superior followed by solirite and biochar mixture (94.33) and solirite alone (83), respectively, and lowest in soil medium (63 to 74). Moreover, spore density and root colonization were significantly and positively correlated (R2 = 0.583, P<0.05) with each other. Solirite alone or mixed with other media improved water holding capacity and porosity with optimum nutrient availability which culminated in better host plant growth and ultimately higher number of AMF propagules.


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Berruti A, Lumini E, Balestrini R and Bianciotto V. 2015. Arbuscular Mycorrhizal Fungi as Natural Biofertilizers: Let’s Benefit from Past Successes. Frontiers in Microbiology 6: 1559.

Black C A. 1965. Methods of soil analysis. Part 1. Physical and Mineralogical Methods. American Society of Agronomy, Inc., Madison (Wisconsin).

Borghezan M, Moraes L K A de, Moreira F M and Silva A L da. 2003. Propagação in vitro e avaliação de parâmetros morfofisiológicos de porta-enxertos de videira. Pesquisa Agropecuária Brasileira 38: 783–89.

Caravaca F, Barea J M, Figueroa D and Roldán A. 2002. Assessing the effectiveness of mycorrhizal inoculation and soil compost addition for enhancing reafforestation with Olea europaea subsp. sylvestris through changes in soil biological and physical parameters. Applied Soil Ecology 20: 107–18.

Chen S, Zhao H, Zou C, Li Y, Chen Y, Wang Z, Jiang Y, Liu A, Zhao P, Wang M and Ahammed G J. 2017. Combined inoculation with multiple arbuscular mycorrhizal fungi improves growth, nutrient uptake and photosynthesis in cucumber seedlings. Frontiers in Microbiology 8: 2516.

Datta P and Kulkarni M. 2012. Arbuscular mycorrhizal fungal diversity in sugarcane rhizosphere in relation with soil properties. Notulae Scientia Biologicae 4: 66.

Gaur A and Adholeya A. 2000. Effects of the particle size of soil-less substrates upon AM fungus inoculum production. Mycorrhiza 10: 43–48.

Gerdemann J W and Nicolson T H. 1963. Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Transactions of the British Mycological Society 46: 235–44.

Gosling P, Hodge A, Goodlass G and Bending G D. 2006. Arbuscular mycorrhizal fungi and organic farming. Agriculture, Ecosystems and Environment 113: 17–35.

Jansa J, Wiemken A and Frossard E. 2006. The effects of agricultural practices on arbuscular mycorrhizal fungi. Geological Society 266: 89–115.

Johnson N C, Angelard C, Sanders I R and Kiers E T. 2013. Predicting community and ecosystem outcomes of mycorrhizal responses to global change. Ecology Letters 16: 140–53.

Kumar A, Choudhary A K, Suri V K and Rana K S. 2016. AM fungi lead to fertilizer phosphorus economy and enhanced system productivity and profitability in okra (Abelmoschus esculentus L.)–pea (Pisum sativum L.) cropping system in Himalayan acid Alfisol. Journal of Plant Nutrition 39: 1380–90.

Lindsay W L and Norvell W A. 1978. Development of a DTPA soil test for Zinc, Iron, Manganese, and Copper 1. Soil Science Society of America Journal 42: 421.

Maitra P, Al-Rashid J, Barman N C, Khan M, Morshed N, Mandal D, Rasul N M, Chowdhury A, El-Sappah A H and Li J. 2021. sand particle size and phosphorus amount affect rhizophagus irregularis spore production using In vitro propagated spore as a starter inoculum in rhizosphere of maize (Zea mays) plantlets. Journal of Fungi 7(10): 846.

Moreira F M S and Siqueira J O Micorrizas. 2002. Microbiologia e Bioquímica do Solo. UFLA: Lavras, p. 473–539.

Newman E I. 1966. A method of estimating the total length of root in a sample. Journal of Applied Ecology 3: 139.

Olsen S R, Cole C V, Watanabe F S and Dean L A. 1954. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. Circular of United States Department of Agriculture pp. 939.

Piper C S. 1966. Soil and Plant Analysis. Hans Publishers, Bombay.

Řezáčová V, Slavíková R, Zemková L, Konvalinková T, Procházková V, Šťovíček V, Hršelová H, Beskid O, Hujslová M, Gryndlerová H and Gryndler M. 2018. Mycorrhizal symbiosis induces plant carbon reallocation differently in C3 and C4 Panicum grasses. Plant and Soil 425: 441–56.

Singh B P and Sainju U M. 1998. Soil physical and morphological properties and root growth. Horticultural Science 33: 966–71.

Sonika C, Sunita K, Neena B and Ashok A. 2013. Inoculum production of Acaulospora laevis using fresh and decomposed apple pomace as substrate. International Journal of Biological Sciences 2: 32–6.

Subbiah B V and Asija C L. 1956. A rapid procedure for the estimation of available nitrogen in soils. Current Science 25: 32.

Tennant D A. 1975. Test of a modified line intersect method of estimating root length. Journal of Ecology 63: 995.









How to Cite

PARIHAR, M., RAKSHIT, A., RANA, K., TIWARI, G., JATAV, S. S., & RAM, H. (2022). Evaluation of various substrates for mycorrhizal inoculum production. The Indian Journal of Agricultural Sciences, 92(1), 31-35. https://doi.org/10.56093/ijas.v92i1.120825