Molecular variability of Macrophomina phaseolina isolates using SSR marker

PRIYANKA PRIYANKA; A C MATHUR; RIDHI SHANKAR SHARMA; R K BAGRI; A K MEENA

doi:10.56093/ijas.v92i1.120874

Authors

PRIYANKA PRIYANKA Rajasthan Agricultural Research Institute, Durgapura, SKNAU, Jobner, Rajasthan 303 329, India
A C MATHUR Rajasthan Agricultural Research Institute, Durgapura, SKNAU, Jobner, Rajasthan 303 329, India
RIDHI SHANKAR SHARMA Rajasthan Agricultural Research Institute, Durgapura, SKNAU, Jobner, Rajasthan 303 329, India
R K BAGRI Rajasthan Agricultural Research Institute, Durgapura, SKNAU, Jobner, Rajasthan 303 329, India
A K MEENA Rajasthan Agricultural Research Institute, Durgapura, SKNAU, Jobner, Rajasthan 303 329, India

https://doi.org/10.56093/ijas.v92i1.120874

Keywords:

Clusterbean, Genetic diversity, Macrophomina phaseolina, Molecular variability, SSR markers

Abstract

An experiment was conducted at RARI, Durgapura, SKNAU, Jobner during kharif 2020 to analyze the genetic diversity of the 12 Macrophomina phaseolina isolates by using simple sequence repeat (SSR) markers. The SSR primers were tested for amplification of genomic DNA of Macrophomia phaseolina isolates. Total five primers of MB series were screened against twelve Macrophomia phaseolina isolates. All primes amplified scorable banding pattern. Level of polymorphism for SSR analysis ranged between 60â€“85.7%. The similarity matrix indicated that 12 isolates exhibited 42.82â€“100% similarity coefficient. The SSR analysis shows high molecular variability among the isolates grouped into five major clusters A, B, C, D and E. In the similarity matrix Mp-DPR was found to have higher value of similarity coefficient (1), whereas Mp-JSM was found to have lower value if similarity coefficient (0.42).

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