Molecular variability of Macrophomina phaseolina isolates using SSR marker
![](/public/icon/gor.png)
![](/public/icon/pdf.png)
Keywords:
Clusterbean, Genetic diversity, Macrophomina phaseolina, Molecular variability, SSR markersAbstract
An experiment was conducted at RARI, Durgapura, SKNAU, Jobner during kharif 2020 to analyze the genetic diversity of the 12 Macrophomina phaseolina isolates by using simple sequence repeat (SSR) markers. The SSR primers were tested for amplification of genomic DNA of Macrophomia phaseolina isolates. Total five primers of MB series were screened against twelve Macrophomia phaseolina isolates. All primes amplified scorable banding pattern. Level of polymorphism for SSR analysis ranged between 60–85.7%. The similarity matrix indicated that 12 isolates exhibited 42.82–100% similarity coefficient. The SSR analysis shows high molecular variability among the isolates grouped into five major clusters A, B, C, D and E. In the similarity matrix Mp-DPR was found to have higher value of similarity coefficient (1), whereas Mp-JSM was found to have lower value if similarity coefficient (0.42).Downloads
References
Kumar D and Rodge A B. 2012. Status, scope and strategies of arid legumes research in India: A Review. Journal of Food Legumes 25: 255–72.
Kumar K and Singh J. 2000. Location, survival, transmission and management of seed borne, Macrophomina phaseolina causing charcoal rot in soybean. Annals of Plant Protection Sciences 8: 44–46.
Lohar P, Mane SS and Walunj P. 2021. Molecular variability among different isolates of Fusarium solani causing root rot in chickpea by SSR marker. International Journal of Chemical Studies 9(3): 241–45.
Murray M G and Thompson W F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8: 4321–26. DOI: https://doi.org/10.1093/nar/8.19.4321
Rangaswami G and Mahadevan A. 2004. Diseases of Crop Plants in India, 4th edn. New Delhi, PHI Learning Private Limited, pp 275–78.
Rohlf F J. 2000. NTSYS- pc Numerical taxonomy and multivariate analysis System. Exeter Biological Software, Setauket, New York. 1:80
Sharma V K, Gaur R B and Bisnoi H R. 2004. Cultural, morphological and physiological variability in Macrophomina phaseolina. Journal of Mycology and Plant Pathology 34: 532–34.
Walunj P, Mane S S, Tatte R, Lohar P and Dhepe D. 2018. Molecular variability in isolates of Rhizoctonia bataticola causing root rot in chickpea by SSR marker. International Journal of Chemical Studies 6(5): 849–52.
Downloads
Submitted
Published
Issue
Section
License
The copyright of the articles published in The Indian Journal of Agricultural Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.