Ploidy study of chrysanthemum (Chrysanthemum morifolium) through PMCâ€™s method

ANU BALA; MADHU BALA; PUJA SRIVASTAVA; VIKRANT KHARE

doi:10.56093/ijas.v92i3.122735

Authors

ANU BALA Punjab Agricultural University, Ludhiana, Punjab 141 004, India
MADHU BALA Punjab Agricultural University, Ludhiana, Punjab 141 004, India
PUJA SRIVASTAVA Punjab Agricultural University, Ludhiana, Punjab 141 004, India
VIKRANT KHARE Punjab Agricultural University, Ludhiana, Punjab 141 004, India

https://doi.org/10.56093/ijas.v92i3.122735

Keywords:

Carnoyâ€™s fixative, Chrysanthemum, Genotypes, Ploidy, Pollen mother cells, Polyploidy

Abstract

Determination of the ploidy level of plants is utmost important for identification of species, selection of suitable parental lines and also to identify the relationships between species to determine the evolutionary patterns. In chrysanthemum species, chromosomes often have different degrees of differentiation, both between and within species. Total 30 genotypes of Chrysanthemum (Chrysanthemum morifolium Ramat.) were used to study the cytology in the Department of Floriculture and Landscaping and Department of Plant Breeding and Genetics, PAU, Ludhiana during 2019 with the objective to find out the genetic variation among the diverse genotypes of chrysanthemum. The ploidy level of 30 chrysanthemum genotypes was studied through meiotic study of pollen mother cells and for this study of meiotic chromosomes, stage and timings of bud collection is very much important. A range of ploidy level was encountered in chromosomal study of selected chrysanthemum germplasm and ploidy level was 2n= 52â€“116. This study indicated that polyploidy variation was found in all varieties of chrysanthemum selected for study. The elucidation of ploidy level of various crops and species provides foundation for understanding their genetic background, therefore, the results of present study will be further helpful for germplasm conservation of chrysanthemum flower crop.

Downloads

Download data is not yet available.

References

Acquaah G. 2007. Principles of plant genetics and breeding. Wiley-Blackwell, Malden.

Bala A, Bala M and Khare V. 2020. A review on cytological study in Chrysanthemum species. Journal of Pharmacognosy and Phytochemistry 9(5): 549–53.

Bennett M D and Leitch I J. 2005. Plant genome size research: a field in focus. Annals of Botany 95: 1–6. DOI: https://doi.org/10.1093/aob/mci001

Chang L I, Chen S, Chen F, Zhen L and Fang W. 2009. Karyo morphological studies on Chinese pot chrysanthemum genotypes with large inflorescences. Agricultural Science of China 8(7): 793–802. DOI: https://doi.org/10.1016/S1671-2927(08)60280-1

Chen L, Lou Q, Zhuang Y, Chen J, Zhang X and Wolukau J N. 2007. Cytological diploidization and rapid genome changes of the newly synthesized allotetraploids Cucumis × hytivus. Planta 225: 603–14. DOI: https://doi.org/10.1007/s00425-006-0381-2

Chen Z. 2010. Molecular mechanisms of polyploidy and hybrid vigor. Trends in Plant Science 15: 57–71. DOI: https://doi.org/10.1016/j.tplants.2009.12.003

Comai L. 2005. The advantages and disadvantages of being polyploid. Nature Reviews Genetics 6: 836–46. DOI: https://doi.org/10.1038/nrg1711

Dai S L, Chen J Y and Li W B. 1998. Application of RAPD analysis in the study on the origin of Chinese cultivated chrysanthemum. Acta Botanica Sinica 40: 1053–59.

Darlington C D. 1973. Chromosome botany and origins of cultivated plants, 161p. George Allen and Unwin Ltd, London.

Dowrick G J. 1953. The chromosomes of chrysanthemum (II). Garden varieties. Heredity 7: 59–72. DOI: https://doi.org/10.1038/hdy.1953.5

Endo N. 1969a. The chromosome survey on the cultivated chrysanthemums, Chrysanthemum morifolium Ramat. I. On DOI: https://doi.org/10.2503/jjshs.38.343