Identification of BCMV resistant germplasm in mungbean (Vigna radiata) using serological and molecular diagnostics

D D DEEPIKA; KULDEEP TRIPATHI; ABHISHEK G J; NEETA SINGH; RUCHI SHARMA; VAISHALI DUTTA SHARMA; A K MAURYA; V CELIA CHALAM

doi:10.56093/ijas.v93i1.123053

Authors

D D DEEPIKA ICAR-Indian Agricultural Research Institute, New Delhi
KULDEEP TRIPATHI ICAR-National Bureau of Plant Genetic Resources, New Delhi, Delhi 110 012, India
ABHISHEK G J ICAR-Indian Agricultural Research Institute, New Delhi
NEETA SINGH ICAR-National Bureau of Plant Genetic Resources, New Delhi, Delhi 110 012, India
RUCHI SHARMA ICAR-National Bureau of Plant Genetic Resources, New Delhi, Delhi 110 012, India
VAISHALI DUTTA SHARMA ICAR-National Bureau of Plant Genetic Resources, New Delhi, Delhi 110 012, India
A K MAURYA ICAR-National Bureau of Plant Genetic Resources, New Delhi, Delhi 110 012, India
V CELIA CHALAM ICAR-National Bureau of Plant Genetic Resources, New Delhi, Delhi 110 012, India

https://doi.org/10.56093/ijas.v93i1.123053

Keywords:

BCMV, ELISA, Germplasm, Resistance, RT-PCR, TEM

Abstract

Diverse germplasm of mungbean serves as a source of resistant gene, screening of germplasm for BCMV resistance will provide resistance source. So, in 2018–2019 a total of 65 diverse mungbean accessions along with susceptible and resistant checks were screened against BCMV under natural conditions in the field and artificial inoculation under controlled conditions. Transmission electron microscopy, per cent disease incidence, serological Direct antigen coating- Enzyme-linked immunosorbent assay (DAC-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) diagnostics were used to detect and identify the BCMV resistant accessions. The study revealed that a total of 29 accessions were found to be immune to BCMV infections. However, these accessions need to be further evaluated in BCMV disease hotspots and under multilocation trials. Therefore, in the present study, accessions that were immune to BCMV may serve as valuable donors for the BCMV resistant mungbean breeding programme.

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