Effect of priming treatments on germination and seedling growth of artificially aged seed of guava (Psidium guajava)

Abstract views: 184 / PDF downloads: 132


  • NEHA SHARMA Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
  • J R SHARMA Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
  • ARVIND MALIK Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
  • AMIT SHARMA Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
  • VIKASH KUMAR Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
  • RITU YADAV Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
  • AJAY KUMAR Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India



Artificially ageing, Guava, Germination, Priming, Seed


An experiment was conducted to study the effect of priming treatments on artificially aged seed germination and seedling growth of guava (Psidium guajava L.) at Department of Seed Science and Technology, CCS Haryana Agriculture University, Hisar during 2019–20. It is comprised 21 treatments in complete randomize design with three replications. There were five priming methods with different treatments under study i.e. tap water, GA3 (at 500, 700 and 1000 ppm concentration), KNO3 (at 0.5 and 1% concentration), thiourea (at 0.5 and 1% concentration) and HCl (at 5% and 10% for 2 and 4 min). Duration of tap water, GA3, KNO3 and thiourea soaking was kept 24 and 48 hours. The study revealed that priming the artificially aged seeds in KNO3 greatly increased germination, especially soaking of artificially aged seeds in KNO3 at 1% for 48 h which shows up to 45.0% germination. Parameter like germination%, mean germination time, seedling length, seedling dry weight and seedling vigour were positively affected by all the priming treatments. Artificially aged seed viability decreased but the standard germination efficiently increased due to priming treatments. KNO3 at 1% for 48 h improved germination and resulted in maximum dry weight of seedling (206.0 mg), seedling length (4.16 cm) and vigour index I and II (187.0 and 9271.0) in laboratory. Artificially aged seeds without any priming treatments showed poor growth and germination.


Download data is not yet available.


Abdelgadir H A, Kulkarni M G, Arruda M P and Van S J. 2012. Enhancing seedling growth of Jatrophacurcas - A potential oil seed crop for biodiesel. South Africa Journal of Botany 78: 88–95. DOI: https://doi.org/10.1016/j.sajb.2011.05.007

Abdul-Baki A A and Anderson J D. 1973. Vigour determination in soyabean seed by multiple criteria. Crop Science 13: 630–33. DOI: https://doi.org/10.2135/cropsci1973.0011183X001300060013x

Akbudak N and Bolkan H. 2010. Diagonistic methods for predicting tomato seedling emergence. Journal Food Agriculture Enviornment 8(1): 170–74.

Anonymous. 2020. Indian Horticulture Database, National Horticulture Board, Gurgaon, Haryana, India.

Baskin C C. 2003. Breaking physical dormancy in seed- focusing on the lens. New Phytologist 158: 227–38. DOI: https://doi.org/10.1046/j.1469-8137.2003.00751.x

Bray C M. 1995. Biochemical processes during the osmopriming of seeds. Seed development and germination, pp. 767–89. Kigel J, Galili G (Eds). New York, Marcel Dekker. DOI: https://doi.org/10.1201/9780203740071-28

Bewley J D and Black M. 1982. Physiology and biochemistry of seeds in relation to germination. Viability, Dormancy and Environmental control, pp. 268–73. Bewley J D and M Black (Eds). Springer-Verlag, UK. DOI: https://doi.org/10.1007/978-3-642-68643-6

Demer I and Mavi K. 2004. The effect of priming on seedling emergence of differentially matured water melon (Citrulluslanatus (Thumb.) matsum and nakai. Seed Science Horticulture 102: 467–73. DOI: https://doi.org/10.1016/j.scienta.2004.04.012

Deshraj. 2002. ‘Studies on viability and vigour in coriander (Coriandrum sativum L.)’. MSc Thesis, CCSHAU, Hisar.

Farooq M, Basra S M A, Wahid A, Ahmad N and Saleem B A. 2009. Improving the drought tolerance in rice (Oryza sativa L.) by exogenous application of salicylic acid. Journal of Agronomy and Crop Science 195: 237–46. DOI: https://doi.org/10.1111/j.1439-037X.2009.00365.x

Golezani K M, Aliloo A A, Valizadeh M and Moghaddam M. 2008. Effect of different priming on seed invigoration and seedling establishment of lentil (lens culinaris Medik). Journal of Agriculture and Environment 6(2): 222–26.

Khan M M, Iqbal M J and Abbas M. 2005. Loss of viability correlates with membrane damage in aged turnip (Brassica rapa) seeds. Seed Science and Technology 33(2): 517–20. DOI: https://doi.org/10.15258/sst.2005.33.2.26

Koehler K H, Voigt B, Spittler H and Schelenz M. 1997. Biochemical events after priming and priming of seeds. Basic and Applied Aspects of Aeed Biology, pp. 531–36. Springer. DOI: https://doi.org/10.1007/978-94-011-5716-2_58

Kumar T R B. 2019. Studies on influence of different seed treatments on dormancy breaking in Aonla (Phyllanthus embolic L.) Journal of Pharmacognosy and Phytochemistry 2: 131–33.

Lay P, Baswaraju G V, Sarika G and Amrutha N. 2013. Effects of seed treatments to enhance seed quality of papaya (carica papaya L.) cv. Surya. Greener journal of biomedical and health science 2(2): 221–25.

Lara T S, Lira J M S, Rodrigues A C, Rakocevic M and Alvarenga A A. 2014. Potassium nitrate priming affects the activity of nitrate reductase and antioxidant enzymes in tomato germination. Journal of Agriculture Science 6: 72–80. DOI: https://doi.org/10.5539/jas.v6n2p72

Moradi D P, Sharif zadeh F and Janmohammadi M. 2008. Influence of priming techniques on seed germination behavior of maize inbred lines (Zea mays L.). ARPN Journal of Agricultural and Biological Science 3(3): 22–25.

Priestley D A. 1986. Seed aging implications of seed storage and persistence in the soil. Cornell University Press, Ithaca.

Rai M K, Asthana P and jaiswal U. 2010. Biotechnology advances in guava (psidium guajava L). Recent Developments and Prospects for Futher Research. Tree: Structure Functional 24: 1–12. DOI: https://doi.org/10.1007/s00468-009-0384-2

Raj D, Dahiya O S, Arya R K, Yadav A K and Kumar K. 2013. Improvement in germination characteristics in artificially aged seeds of okra (Abelmoschus esculentus) by osmo-conditioning. Indian Journal Agriculture Science 83(7): 699–702.

Shim S I, Moon J C, Jang C S, Raymer P and Kim W. 2008. Effect of potassium nitrate priming on seed germination of seashore paspalum. Horticulture Science 43(7): 2259–62. DOI: https://doi.org/10.21273/HORTSCI.43.7.2259

Singh A, Dahiru R, Musa M and Haliru B S 2014. Effect of osmo priming duration on germination, emergence and early growth of cowpea (Vigna unguiculata L. Walp) in the Sudan savanna Nigeria. International Journal of Agronomy 4 ID 841238:4. DOI: https://doi.org/10.1155/2014/841238

Singhal D K, Kumar V, Tehlan S K and Mor V S. 2017. Evaluation of biochemical changes associated with seed quality under naturally stored conditions in fennel. International Journal of Chemical Studies 5(5): 1800–03.

Sheoran O P, Tonk D S, Kaushik L S, Hasija R C and Pannu R S. 1998. Statistical Software Package for Agricultural Research Workers. Recent Advances in information theory, Statistics and Computer Applications. D S Hooda and R C Hasija Department of Mathematics Statistics, CCS HAU, Hisar, 139–43.

Srinivasan K and Saxena S. 2007. Removal of tenacious glumes enhances the seed germination and seedling vigour in eight Aegilops species for efficient conservation in genebank. Indian Journal of Agricultural Sciences 83(4): 437–40.

Thomas L R, Micheal G B, Ralph S C and Terence M M 2006. Plant Biology: Seeds and Fruit, 6th edn. 14: 213–20.

Tokuhisa D, Dias D C F D S, Alvarenga E M, Hilst P C and Demuner A J. 2007. Compostos fenolicos inibidores da germinacao em sementes de mamao (Carica papaya). Revista Brasileira de Sementes 29: 180–88. DOI: https://doi.org/10.1590/S0101-31222007000300022

Vazirimehr M R, Ganjali H R, Rigi K and Keshtehgar A. 2014. Effect of seed priming on quantitative traits corn. International Journal of Plant Science 4: 134–40

Windauer L, Altun A and Benech Arnold R. 2007. Hydrotime analysis of lesquerella fendleri seed germination responses to priming treatments. Industrial Crops and Products 25: 70–74. DOI: https://doi.org/10.1016/j.indcrop.2006.07.004









How to Cite

SHARMA, N. ., SHARMA, J. R. ., MALIK, A. ., SHARMA, A. ., KUMAR, V. ., YADAV, R. ., & KUMAR, A. . (2022). Effect of priming treatments on germination and seedling growth of artificially aged seed of guava (Psidium guajava). The Indian Journal of Agricultural Sciences, 92(4), 516-520. https://doi.org/10.56093/ijas.v92i4.124008