Biofortification of iron in rice (Oryza sativa) grown in acid soil of Assam, India

##makale.goruntuleme.sayisi##: 172 / ##makale.indirme.sayisi##: 146


  • MADHUSMITA BARUAH Assam Agricultural University, Jorhat, Assam 785 013, India
  • PRIYANKA DAS Assam Agricultural University, Jorhat, Assam 785 013, India
  • NILAY BORAH Assam Agricultural University, Jorhat, Assam 785 013, India
  • SANJAY K CHETIA Regional Agricultural Research Station, Titabar, Jorhat, Assam
  • BORSHA NEOG Assam Agricultural University, Jorhat, Assam 785 013, India



Biofortification, Brown rice iron content, Iron fertilizer


A pot experiment was conducted during 2019 under rain protected condition at Assam Agricultural University, Jorhat to know the effect of two different levels of soil iron (Fe) content on Fe content of rice tissues including grain (brown rice). Soils of initial DTPA extractable Fe content of 159.40±0.51 mg/kg was applied with 50 ppm Ferrous sulfate solution to increase the soil Fe content to 182.35±0.57 mg/kg. Two popular rice varieties, Ranjit and Mahsuri, and one traditional variety Kajoli Chakua were cultivated as winter rice under submerged condition in pots. There was no Fe toxicity symptom in the leaves for the plants grown at two different soil Fe content. At harvesting stage, the concentration of Fe in different tissues of rice were detected in the order: leaves>husk>grain. The Fe content of brown rice was significantly higher (>100% than that of control) in plants grown in soils of higher Fe content. Significant variation in the Fe content of brown rice was observed according to its position on the rachis, being the highest at the top and the lowest at the bottom. Considering initial soil Fe status, application of Fe fertilizer of suitable concentration to the soil was found to be successful in biofortification of Fe in brown rice, leading to increase of Fe content depending on varieties.


Download data is not yet available.


Baruah K K and Bharali A. 2015. Physiological basis of iron toxicity and its management in crops. Recent Advances in Crop Physiology, Vol. 2, pp. 203–24. Singh A L (Ed). Daya Publishing House, New Delhi.

Borah N, Das K N, Bordoloi P K and Goswami J. 2000. Effect of added iron in irrigation water on iron content of rice plant in relation to soil iron and clay content. Annals of Agri Bio Research 5(1): 15–18.

Kok A D, Yoon L L, Sekeli R, Yeong W C, Yusof Z N B and Song L K. 2018. Iron biofortification of rice: Progress and prospects. Rice Crop-Current Developments, pp. 25–44.

Shah F, Khan Z H and Iqbal A (Eds). Intech Open, London, UK.

Krupa K N, Dalawai N, Shashidhar H E and Swamy V H V. 2017. Assessment of iron content and distribution in different parts of rice grain. International Journal of Pure and Applied Biosciences 5(4): 212–20.

Majumder S, Datta K and Datta S K. 2019. Rice fortification: high iron, zinc and vitamin-A to fight against “hidden hunger”. Agronomy 9: 803–25.

Meng F, Wei Y and Yang X. 2005. Iron content and bioavailability in rice. Journal of Trace Elements in Medicine and Biology 18: 333–38.

Saini R, Saini, H S, Dahiya A and Jain S. 2017. Iron content in rice under differential iron treatment. International Journal of Advanced Research 5(7): 794–801.

Singh B R, Timsina Y N, Lind O C, Cagno S and Janssens K. 2018. Zinc and iron concentration as affected by nitrogen fertilization and their localization in wheat grain. Frontiers in Plant Science 9: 307.

Su D, Sultan F, Zhao N, Lei B, Wang F, Pan G and Cheng F. 2014. Positional variation in grain mineral nutrients within a rice panicle and its relation to phytic acid concentration. Journal of Zhejiang University-Science B (Biomedicine and Biotechnology) 15(11): 986–96.

Wheeler E L and Ferrel R E. 1971. A method for phytic acid determination in wheat and wheat fractions. Cereal Chemistry 48: 312–14.

Wong S Y. 1928. Colourimetric determination of iron and haemoglobin in blood. Journal of Biological Chemistry 77(2): 409–12.









How to Cite

BARUAH, M., DAS, P., BORAH, N., CHETIA, S. K., & NEOG, B. (2022). Biofortification of iron in rice (Oryza sativa) grown in acid soil of Assam, India. The Indian Journal of Agricultural Sciences, 92(11), 1399–1401.