Introgression of disease resistance in urdbean (Vigna mungo) by involving its related species

SHAILJA SHARMA; R K MITTAL; H K CHAUDHARY; V K SOOD

doi:10.56093/ijas.v92i11.124619

Authors

SHAILJA SHARMA College of Agriculture, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya (CSKHPKV), Palampur, Himachal Pradesh 176 062, India
R K MITTAL College of Agriculture, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya (CSKHPKV), Palampur, Himachal Pradesh 176 062, India
H K CHAUDHARY College of Agriculture, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya (CSKHPKV), Palampur, Himachal Pradesh 176 062, India
V K SOOD College of Agriculture, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya (CSKHPKV), Palampur, Himachal Pradesh 176 062, India

https://doi.org/10.56093/ijas.v92i11.124619

Keywords:

Cercospora, Hybridity, Interspecific, Morphological, Vigna mungo, V. radiata, V. umbellata

Abstract

Cercospora leaf spots is one of the major foliar diseases of urdbean (Vigna mungo (L.) Hepper) causing a considerable yield loss. Screening of germplasm and induction of mutation has not yielded any source of resistance. Hence, an experiment was conducted at research farm of CSKHPKV Palampur, Himachal Pradesh during 2017–21 for introgression of disease resistance in urdbean. A total of 45 interspecific cross combinations were attempted between urdbean × moongbean [Vigna radiata (L.) R. Wilczek], urdbean × ricebean [Vigna umbellata (Thunb.) Ohwi and H. Ohashi] and urdbean × adzukibean [Vigna angularis (Willd.) Ohwi and H. Ohashi]. Only 12 interspecific crosses were successful. SSRs were used for confirmation of hybridity of the wide crosses. True F1’s were phenotyped for 13 morphological characteristics with respect to parental lines to study the trait expression. Most of the morphological characteristics of the interspecific F1’s were either intermediate to both the parents or resembled to their male parents. Here, we also report the interspecific wide hybridization in Vigna through bridge species crossing strategy where the F1 of urdbean × moongbean was hybridized separately with ricebean and adzukibean. All the interspecific hybrids of urdbean and ricebean showed resistant reaction to Cercospora leaf spots.

Downloads

Download data is not yet available.

References

Abbas G, Hameed A, Rizwan M, Ahsan M, Asghar M J and Iqbal N. 2015. Genetic confirmation of moongbean (Vigna radiata) and mashbean (Vigna mungo) interspecific recombinants. Frontiers in Plant Science 1107(6): 1–7. DOI: https://doi.org/10.3389/fpls.2015.01107

Bhanu A K, Kumar P, Singh M N, Srivastava K and Hemantarajan A. 2017. Assessment of genetic purity of interspecific F1 hybrids involving Vigna radiata and Vigna umbellata. Journal of Experimental Biology and Agricultural Sciences 5(5): 636–43. DOI: https://doi.org/10.18006/2017.5(5).636.643

Bindra S, Mittal R K, Sood V K and Chaudhary H K. 2020. Alien Introgression studies involving Vigna mungo × Vigna umbellata hybridization. International Journal of Current Microbiology and Applied Science 9(02): 268–76. DOI: https://doi.org/10.20546/ijcmas.2020.902.034

Lekhi P, Gill R K, Kaur S and Bains T S. 2017. Generation of interspecific hybrids for introgression of moongbean yellow mosaic virus resistance in Vigna radiata (L.) Wilczek. Legume Research 41(4): 526–31. DOI: https://doi.org/10.18805/LR-3808

Lukoki L, Marechal R and Otoul E. 1980. The wild ancestors of the cultivated beans V. radiata and V. mungo. Bulletin du Jardin Botanique National de Belgique 50: 385–91. DOI: https://doi.org/10.2307/3667837

Mayee C D and Datar V V. 1986. Phytopathometry, pp. 72. Marathwada Agricultural University, Parbhani, Maharashtra, India.

Murray M G and Thompson W F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8: 4321–25. DOI: https://doi.org/10.1093/nar/8.19.4321

Gupta D S, Singh U, Kumar J, Shivay Y S, Dutta A, Sharanagat V S, Katiyar P K and Singh N P. 2020. Estimation and multi-variate analysis of iron and zinc concentration in a diverse panel of urdbean (Vigna mungo L. Hepper) genotypes grown under differing soil conditions. Journal of Food composition and Analysis 93: 119–29. DOI: https://doi.org/10.1016/j.jfca.2020.103605

Pratap A, Das A, Kumar S and Gupta S. 2021. Current prospective on introgression breeding in food legumes. Frontiers in Plant Sciences 11: 589189 doi: 10.3389/fpls.2020.589189. DOI: https://doi.org/10.3389/fpls.2020.589189

Singh B B, Solanki R K, Chaubey B K and Verma P. 2011. Breeding for improvement of warm season food legumes. Biology and Breeding of Food Legumes, pp. 63–80. Pratap A and Kumar J (Eds). CAB International, Oxford press, London. DOI: https://doi.org/10.1079/9781845937669.0063

Zeven A C and De Wet J M J. 1982. Dictionary of cultivated plants and their regions of diversity. Centre for Agricultural Publication and Documentation, Wageningen.