Marker-assisted pyramiding of southern leaf blight resistance QTLs qSLB3.1 and qSLB8.1 in maize (Zea mays)

PRABHMEET KAUR; GURWINDER KAUR; MOHAMMED KYUM; SHABNEEK KAUR; SUTEJ BAINS; PRITI SHARMA; HARLEEN KAUR; SURINDER K SANDHU; YOGESH VIKAL

doi:10.56093/ijas.v92i12.128318

Authors

PRABHMEET KAUR School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana
GURWINDER KAUR School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana 141 004, India
MOHAMMED KYUM Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana
SHABNEEK KAUR School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana 141 004, India
SUTEJ BAINS School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana 141 004, India
PRITI SHARMA School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana 141 004, India
HARLEEN KAUR School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana 141 004, India
SURINDER K SANDHU Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana
YOGESH VIKAL School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana 141 004, India https://orcid.org/0000-0001-5821-9345

https://doi.org/10.56093/ijas.v92i12.128318

Keywords:

Marker-assisted selection, Pyramiding, Quantitative trait loci, Southern leaf blight resistance

Abstract

Southern leaf blight (SLB) is one of the major diseases that cause substantial yield losses in maize (Zea mays L.) worldwide. Stacking broad-spectrum resistance genes/QTLs into prevalent cultivars is the prerequisite for durable disease resistance breeding programme. Therefore, a study was carried at the research farm and molecular biology laboratory of School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab during 2017–21 to introgress SLB resistance QTLs from two donors, viz. CM139 (qSLB3.1) and LM5 (qSLB8.1) into CM140 inbred using marker-assisted backcross breeding (MABB). Crosses were made between CM139 × CM140 and LM5 × CM140 to generate two separate F1s. Each F1 was backcrossed twice to generate BC2F1 progenies. Foreground selection was performed at each step using linked flanking markers to each QTL and also evaluated for SLB resistance. The selected heterozygous BC2F1 plants from each cross were inter-crossed to combine qSLB3.1 and qSLB8.1 in the same genetic background. The F2 population was also evaluated phenotypically for SLB resistance and other morphological traits. A total of 18 plants were obtained having both the QTLs with homozygous donor alleles. The F2 plants having both QTLs and singly in the homozygous state were advanced to generate F3 progenies. The pyramided lines exhibited 29% lesser disease severity than the lines with either QTL. The present results indicated that additive effects of the QTLs for SLB resistance played an important role among these lines. To our best knowledge, this is the first report for the pyramiding of QTLs associated with SLB resistance. The pyramided lines would serve as potential donors in maize breeding programs.

Downloads

Download data is not yet available.

References

Ashikari M and Matsuoka M. 2006. Identification, isolation and pyramiding of quantitative trait loci for rice breeding. Trends in Plant Science 11: 344–50. DOI: https://doi.org/10.1016/j.tplants.2006.05.008

Bai B, He Z H, Asad M A, Lan C X, Zhang Y and Xia X C. 2012. Pyramiding adult-plant powdery mildew resistance QTLs in bread wheat. Crop Pasture Science 63: 606–11. DOI: https://doi.org/10.1071/CP12183

Balint-Kurti P, Zwonitzer J, Pe M, Pea G, Lee M and Cardinal A J. 2008. Identification of quantitative trait loci for resistance to southern leaf blight and days to anthesis in two maize recombinant inbred line populations. Phytopathology 96: 1067–71. DOI: https://doi.org/10.1094/PHYTO-96-1067

Belcher A, Zwonitzer J, Cruz J, Krakowsky M, Chung C, Nelson R, Arellano C and Balint-Kurti P. 2012. Analysis of quantitative disease resistance to southern leaf blight and of multiple disease resistance in maize, using near-isogenic lines. Theoretical Applied Genetics 124: 433–45. DOI: https://doi.org/10.1007/s00122-011-1718-1

Craig J and Fajemisin J M. 1969. Inheritance of chlorotic lesion resistance to Helminthosporium maydis in maize. Plant Disease Report 53: 742–43.

Crossa J, Pérez-Rodríguez P, Cuevas J, Montesinos-Lopez O, Jarquín D, De Los Campos G, Burgueño J and González- Camacho J M. 2017. Genomic selection in plant breeding: methods, models, and perspectives. Trends in Plant Science 22: 961–75. DOI: https://doi.org/10.1016/j.tplants.2017.08.011

Hooda K S, Bagaria P K, Khokhar M, Kaur H and Rakshit S. 2018. Mass Screening Techniques for Resistance to Maize Diseases, pp. 93. ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana.

Kaur K. 2019. ‘Fine mapping of QTL QSlb.pau-3.04 for southern leaf blight resistance in maize (Zea mays L.)’. PhD Thesis, Punjab Agricultural University, Ludhiana.

Kaur S. 2020. ‘Fine mapping of QTL (QSLB8.1 and QSLB9.1) for southern corn leaf blight resistance in maize (Zea mays L.)’. MSc Thesis, Punjab Agricultural University, Ludhiana.

Kaur D, Kaur A, Kaur P I, Grewal M S and Vikal Y. 2013. Analysis of molecular diversity in water stress tolerant and susceptible maize inbred lines. Crop improvement 40: 1–10.

Kaur M, Vikal Y, Kaur H, Pal L, Kaur K and Chawla J S. 2019. Mapping quantitative trait loci associated with southern leaf blight resistance in maize (Zea mays L.). Journal of Phytopathology 167: 591–600. DOI: https://doi.org/10.1111/jph.12849

Kump K, Holland J, Jung M, Wolters P and Balint-Kurti P. 2010. Joint analysis of near isogenic and recombinant inbred line populations yields precise positional estimates for quantitative trait loci. Plant Genome 3: 142–53. DOI: https://doi.org/10.3835/plantgenome2010.05.0011

Kump K L, Bradbury P J, Wisser R J, Buckler E S, Belcher A R, Oropeza-Rosas M A and Holland J B. 2011. Genome-wide association study of quantitative resistance to southern leaf blight in the maize nested association mapping population. Nature Genetics 43: 163–68. DOI: https://doi.org/10.1038/ng.747

Lennon J R, Krakowsky M, Goodman M, Flint-Garcia S and Balint-Kurti P J. 2017. Identification of teosinte alleles for resistance to southern leaf blight in near isogenic maize lines. Crop Science 57: 1973–83. DOI: https://doi.org/10.2135/cropsci2016.12.0979

Li Y X, Chen L, Li C, Bradbury P J, Shi Y S, Song Y and Wang T. 2018. Increased experimental conditions and marker densities identified more genetic loci associated with southern and northern leaf blight resistance in maize. Scientific Reports 8: 6848. DOI: https://doi.org/10.1038/s41598-018-25304-z

Lim S M. 1975. Heterotic effects of resistance in maize to Helminthosporium maydis race O. Phytopathology 65: 1117–20. DOI: https://doi.org/10.1094/Phyto-65-1117

Manjunatha C, Gogoi R, Singh B, Jeevan B, Rai S N and Singh P K. 2019. Phenotypic and physiological characterization of maize inbred lines resistant and susceptible to maydis leaf blight. Indian Phytopathology 72: 217–24. DOI: https://doi.org/10.1007/s42360-019-00117-w

Martins L B, Rucker E, Thomason W, Wisser R J, Holland J B and Balint-Kurti P. 2019. Validation and characterization of maize multiple disease resistance QTL. G3: Genes Genome Genetics 9: 2905–12. DOI: https://doi.org/10.1534/g3.119.400195

Saghai-Maroof H A, Soliman K M, Jorgensen A R and Allard R W.1984. Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Procceedings of National Academic Sciences USA 81: 8014–18. DOI: https://doi.org/10.1073/pnas.81.24.8014

Santa-Cruz J H, Kump K L, Arellano C, Goodman M M, Krakowsky M D, Holland J B and Balint-Kurti P J. 2014. Yield effects of two southern leaf blight resistance loci in maize hybrids. Crop Science 54: 882–94. DOI: https://doi.org/10.2135/cropsci2013.08.0553

Shaner G and Finney R E. 1977. The effect of nitrogen fertilization on the expression of slow-mildewing resistance in Knox wheat. Phytopathology 67: 1051–56. DOI: https://doi.org/10.1094/Phyto-67-1051

Smith D R and Hooker A L.1973. Monogenic chlorotic-lesion resistance in corn to Helminthosporium maydis. Crop Science 13: 330–31. DOI: https://doi.org/10.2135/cropsci1973.0011183X001300030013x

Sun X, Qi X, Wang W, Liu X, Zhao H, Wu C, Chang X and Zhang M. 2020. Etiology and symptoms of maize leaf spot caused by Bipolaris spp. in Sichuan, China. Pathogen 9: 229–46. DOI: https://doi.org/10.3390/pathogens9030229

Thompson D and Bergquist R. 1984. Inheritance of mature plant resistance to Helminthosporium maydis race O in maize. Crop Science 24: 807–11. DOI: https://doi.org/10.2135/cropsci1984.0011183X002400040042x

Tyagi S, Mir R R, Kaur H, Chhuneja P, Ramesh B, Balyan H S and Gupta P K. 2014. Marker-assisted pyramiding of eight QTLs/ genes for seven different traits in common wheat (Triticum aestivum L.). Molecular Breeding 34: 167–75. DOI: https://doi.org/10.1007/s11032-014-0027-1

Ullstrup A J. 1972. The impacts of the southern corn leaf blight epidemis of 1970–71. Annual Review of Phytopathology 10: 37–50. DOI: https://doi.org/10.1146/annurev.py.10.090172.000345

Vasmatkar P, Kaur K, Pannu P P S, Kaur G and Kaur H. 2019. Unraveling the metabolite signatures of maize genotypes showing differential response towards southern corn leaf blight by 1H-NMR andFTIR spectroscopy. Physiological and Molecular Plant Pathology 108: 101441. DOI: https://doi.org/10.1016/j.pmpp.2019.101441

Wei J K, Lui K M, Chen J P, Luo P C and Stadelmann O Y. 1988. Pathological and physiological identification of race C of Bipolaris maydis in China. Phytopathology 78: 550–54. DOI: https://doi.org/10.1094/Phyto-78-550

Yang T, Zhang S, Zhao J, Liu Q, Huang Z, Mao X, Dong J and Wang X. 2016. Identification and pyramiding of QTLs for cold tolerance at the bud bursting and the seedling stages by use of single segment substitution lines in rice (Oryza sativa L.). Molecular Breeding 36: 96. DOI: https://doi.org/10.1007/s11032-016-0520-9

Zwonitzer J, Coles N, Krakowsky M, Arellano C, Holland J, McMullen M, Pratt R and Balint-Kurti P. 2010. Mapping resistance quantitative trait loci for three foliar diseases in maize recombinant inbred line population-Evidence for multiple disease resistance. Phytopathology 100: 72–79. DOI: https://doi.org/10.1094/PHYTO-100-1-0072