Screening lentil (Lens culinaris) RIL population for high yield and aluminium toxicity tolerance under acidic field and hydroponic conditions

MAYURAKSHEE  MAHANTA; NOREN SINGH  KONJENGBAM; REGINAH  PHEIRIM; ANDREAN ALLWIN  LYNGDOH

doi:10.56093/ijas.v94i6.132449

Authors

MAYURAKSHEE MAHANTA College of Post Graduate Studies in Agricultural Sciences (Central Agricultural University, Imphal, Manipur), Umiam, Meghalaya 793 103, India
NOREN SINGH KONJENGBAM College of Post Graduate Studies in Agricultural Sciences (Central Agricultural University, Imphal, Manipur), Umiam, Meghalaya 793 103, India https://orcid.org/0000-0002-7474-186X
REGINAH PHEIRIM College of Post Graduate Studies in Agricultural Sciences (Central Agricultural University, Imphal, Manipur), Umiam, Meghalaya 793 103, India
ANDREAN ALLWIN LYNGDOH College of Post Graduate Studies in Agricultural Sciences (Central Agricultural University, Imphal, Manipur), Umiam, Meghalaya 793 103, India

https://doi.org/10.56093/ijas.v94i6.132449

Keywords:

Aluminium toxicity, High yield, Hydroponics, Lentil, Tolerance

Abstract

The present study was carried out during winter (rabi) season of 2020–21 at two locations namely the experimental field of College of Post Graduate Studies in Agricultural Sciences (Central Agricultural University, Imphal, Manipur), Umiam, Meghalaya and Agro-forestry experimental plot of ICAR-North-Eastern Hill Region, Meghalaya followed by hydroponics screening during winter (rabi) season of 2021–22. The experiments involved screening of a recombinant inbred line (RIL) population of lentil (Lens culinaris Medik) obtained from a cross between BM-4 (Al sensitive parent) and L-4602 (Al tolerant parent) for high yield and Al (aluminium) toxicity tolerance through evaluation in the acidic field conditions, character association studies and root growth studies under hydroponics having toxic Al concentration of 148 µM. Highly significant variance due to genotypes revealed presence of sufficient variability for all the traits except number of seeds/pod (SP). Among the characters, high Hbs2 associated with high GA% were recorded in number of primary branches/plant (NB), plant height (PH) and 100-seed weight (SW). Path analysis revealed that, number of pods/plant (PP) (0.840) had the greatest direct effects in influencing seed yield/plant (SYPP), followed by biological yield/plant (BYPP) (0.795), number of seeds/pod (SP) (0.474), number of primary branches/plant (NB) (0.309) and harvest index (HI) (0.307). Correlation studies among root and shoot parameters under hydroponic studies revealed significant correlation between root dry weight (RDW) and shoot dry weight (SDW); shoot fresh weight (SFW) and root fresh weight (RFW); total root length (TRL) and surface area (SA); and haematoxylin stain score (STNS) and root regrowth (RRG). Based on mean performance of SYPP and attributing traits, combined with root growth studies under hydroponics, the high yielding and Al toxicity tolerant genotypes identified were LRIL-37, LRIL-22, LRIL-68, LRIL-96 and LRIL-97. In addition to serving as parents in hybridization programmes, these genotypes may undergo additional evaluation in multiple environments prior to final release in an effort to enhance performance.

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References

Abate E, Hussein S, Amelework A, Shaff J E, Laing M, Tadele Z and Mengistu F. 2022. Investigation of Al-toxicity tolerance in tef (Eragrostis tef) under hydroponic system using root growth measurement and haematoxylin staining methods. Australian Journal of Crop Science 16(8): 1047–059.

Abbas G, Asghar M J, Shahid M, Hussain J, Akram M and Ahmad F. 2019. Yield performance of some lentil genotypes over different environments. Agrosystems, Geosciences and Environment 2(1): 1–3.

Anonymous. 2020. Agricultural Statistics at a Glance. Economics and Statistics Division, Department of Agriculture and Farmers Welfare, Ministry of Agriculture and Farmers Welfare, Government of India.

Anonymous. 2022. Export Import Data Bank. Department of Commerce, Ministry of Commerce and Industry. Government of India. jkkihttps://tradestat.commerce.gov.in/eidb/ecomcntq. asp. Retrieved on 15.7.22

Chowdhury M M, Haque M A, Malek M A, Rasel M and Ahamed K U. 2019. Genetic variability, correlation and path coefficient analysis for yield and yield components of selected lentil (Lens culinaris Medik) genotypes. Fundamental and Applied Agriculture 4(2): 769–76.

De Macedo C E C, Van Sint Jan V, Kinet J M and Lutts S. 2009. Effects of aluminium on root growth and apical root cells in rice (Oryza sativa L.) cultivars. Reliability of screening tests to detect Al resistance at the seedling stage. Acta Physiologiae Plantarum 31: 1255–262.

Dugassa A, Legesse H and Geleta N. 2014. Genetic variability, yield and yield associations of lentil (Lens culinaris Medik) genotypes grown at Gitilo Najo, western Ethiopia. Science, Technology and Arts Research 3(4): 10–18.

Hassan M S, Raslan M A E, Kalhy G M and Ali M A. 2021. Evaluation and path analysis for yield and its components in some genotypes of lentil (Lens culinaris Medikus) under upper Egypt condition. SVU-International Journal of Agricultural Sciences 3(2): 37–51.

Kochian L V, Pineros M A and Hoekenga O A. 2005. The physiology, genetics and molecular biology of plant aluminum resistance and toxicity. Plant and Soil 274: 175–95.

Ma Q, Yi R, Li L, Liang Z, Zeng T, Zhang Y and Nian H. 2018. GsMATE encoding a multidrug and toxic compound extrusion transporter enhances aluminum tolerance in Arabidopsis thaliana. BMC Plant Biology 18(1): 1–10.

Mahajan R C, Wadikar P B, Pole S P and Dhuppe M V. 2011. Variability, correlation and path analysis studies in sorghum. Research Journal of Agricultural Sciences 2(1): 101–03.

Majumdar S, Behera U K and Wanniang S. 2022. Acid soil management in north-eastern region of India. Indian Farming 72(3): 35–42.

Pant K R, Gurung S B, Dhami N B, Shrestha J, Aryal L and Darai R. 2019. Agro-morphological traits variability of lentil genotypes. Nepalese Journal of Agricultural Sciences 18: 108–14.

Peoples M B, Brockwell J, Herridge D F, Rochester I J, Alves B J R, Urquiaga S and Jensen E S. 2009. The contributions of nitrogen-fixing crop legumes to the productivity of agricultural systems. Symbiosis 48(1): 1–17.

Polle E, Konzak C F and Kattrick J A. 1978. Visual detection of aluminum tolerance levels in wheat by hematoxylin staining of seedling roots. Crop Science 18(5): 823–27.

Satpathy S and Debnath S. 2020. Genetic analysis of yield and its attributing traits in lentil. Journal of Pharmacognosy and Phytochemistry 9(2): 713–18.

Sharma R, Chaudhary L, Kumar M and Panwar N. 2022. Analysis of genetic parameters and trait relationship for seed yield and its attributing components in lentil (Lens culinaris Medik.). Legume Research-An International Journal 45(11): 1344–350.

Silva S, Pinto-Carnide O, Martins-Lopes P, Matos M, Guedes- Pinto H and Santos C. 2010. Differential aluminium changes on nutrient accumulation and root differentiation in an Al sensitive vs. tolerant wheat. Environmental and Experimental Botany 68(1): 91–98.

Singh D and Raje R S. 2011. Genetics of aluminium tolerance in chickpea (Cicer arietinum). Plant Breeding 130(5): 563–68.

Singh C K, Singh D, Tomar R S S, Karwa S, Upadhyaya K C and Pal M. 2018. Molecular mapping of aluminium resistance loci based on root regrowth and Al-induced fluorescent signals (callose accumulation) in lentil (Lens culinaris Medikus). Molecular Biology Reports 45: 2103–113.

Singh D, Dikshit H K and Singh R. 2012. Variation of aluminium tolerance in lentil (Lens culinaris Medik.). Plant Breeding 131(6): 751–61.

Singh D, Pal M, Singh C K, Taunk J, Jain P, Chaturvedi A K, Maurya S, Karwa S, Singh R, Tomar R S S, Nongthambam R, Chongtham N and Singh M P. 2016. Molecular scanning and morpho-physiological dissection of component mechanism in Lens species in response to aluminium stress. PLoSONE 11(7): 1–30.

Singh D, Pal M, Singh R, Singh C K and Chaturvedi A K. 2015. Physiological and biochemical characteristics of Vigna species for Al stress tolerance. Acta Physiologiae Plantarum 37: 1–13.

Singh R K and Chaudhary B D. 1985. Biometrical Method in Quantitative Genetics Analysis. Kalyani Publishers, New Delhi. Singh U and Srivastava R K. 2013. Genetic variability, heritability, interrelationships association and path analysis in lentil (Lens culinaris Medik.). Trends in Biosciences 6(3): 277–80.

Singh D and Choudhary A K. 2010. Inheritance pattern of aluminum tolerance in pea. Plant Breeding 129(6): 688–92.

Stodart B J, Raman H, Coombes N and Mackay M. 2007. Evaluating landraces of bread wheat Triticum aestivum (L.) for tolerance to aluminium under low pH conditions. Genetic Resources and Crop Evolution 54: 759–66.

Takele E, Mekbib F and Mekonnen F. 2022. Genetic variability and characters association for yield, yield attributing traits and protein content of lentil (Lens Culinaris Medikus) genotype in Ethiopia. CABI Agriculture and Bioscience 3(1): 1–14.

Urbano G, Porres J M, Frias J and Vidal-Valverde C. 2007. Nutritional value. Lentil, pp. 47–93. Yadav S S, McNeil D L and Stevenson P C (Eds). Springer, Dordrecht.

Verma S K, Panwar R K, Gaur A K, Bisht C, Deep H, Yadav H and Chauhan C. 2022. An integrated approach for simultaneous selection of stable and high yielding genotypes in lentil (Lens culinaris Medikus). Legume Research-An International Journal 1: 8.