Phytochemical profiling, antioxidant capacities and anthocyanin compositions of the pigmented rice (Oryza sativa) of north-east India
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Keywords:
Anthocyanins, Antioxidants, DPPH, HPLC, Pigmented riceAbstract
The association between the consumption of pigmented rice (Oryza sativa L.) and the improvement of human health is generating a great deal of interest among the researchers. An experiment was conducted during 2020 and 2021 at Assam Agricultural University, Jorhat, Assam to study the phytochemical profiling, antioxidant capacities and anthocyanin compositions of the 14 pigmented rice genotypes. The results were compared with the well-known traditional pigmented rice variety from Manipur, Poreiton Chakhao. The total phenolic content, total flavonoid content and total anthocyanin content ranged from 37.101 to 493.611 mg gallic acid equivalent/100 g, 53.316 to 151.667 mg quercetin equivalent/100 g and 1.006 to 13.904 mg cyanidin-3-chloride equivalent/100 g in the studied rice genotypes. The amount of rice showing 50% DPPH free radical scavenging activity (IC 50 value) ranged from 6.610 to 29.376 mg. The HPLC analysis revealed presence of both cyanidin-3-glucoside and peonidin-3-glucoside in the black pigmented rice genotypes, viz. TTB Black Rice 7, Chakhao-1, Chakhao-2 and TTB Black Rice 11. However, in the red rice genotypes analysed (Balam and Nepali Chakuwa), these two anthocyanins were not detected.
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Abdel-Aal E-SM and Hucl P. 1999. A rapid method for quantifying total anthocyanins in blue aleurone and purple pericarp wheats. Cereal Chemistry 76: 350–54. DOI: https://doi.org/10.1094/CCHEM.1999.76.3.350
Abdel-Aal ESM, Young J C and Rabalski I. 2006. Anthocyanin composition in black, blue, pink, purple, and red cereal grains. Journal of Agricultural and Food Chemistry 54: 4696–4704. DOI: https://doi.org/10.1021/jf0606609
Achparaki M, Thessalonikeos E, Tsoukali H, Mastrogianni O, Zaggelidou E and Chatzinikolaou F. 2012. https://www.intechopen.com/books/advanced-biometric-technologies/liveness-detection-in-biometrics%0A.
Asem I D, Imotomba R K, Mazumder P B and Laishram J M. 2015. Anthocyanin content in the black scented rice (Chakhao): Its impact on human health and plant defense. Symbiosis 66: 47–54. DOI: https://doi.org/10.1007/s13199-015-0329-z
Bhuvaneswari S, Gopala Krishnan S, Bollinedi H, Saha S, Ellur R K, Vinod K K, Singh I M, Prakash N, Bhowmick P R, Nagarajan M, Singh N R and Singh A K. 2020. Genetic Architecture and anthocyanin profiling of aromatic rice from Manipur reveals divergence of chakhao landraces. Frontiers in Genetics 11: 570731. DOI: https://doi.org/10.3389/fgene.2020.570731
Brand-Williams W, Cuvelier M E and Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology 28: 25–30. DOI: https://doi.org/10.1016/S0023-6438(95)80008-5
Chaudhary R C. 2003. Speciality rices of the world: Effect of WTO and IPR on its production trend and marketing. Journal of Food Agriculture and Environment 1(2): 34–41.
Hu C, Zawistowski J, Ling W and Kitts D D. 2003. Black rice (Oryza sativa L.) pigmented fraction suppresses both reactive oxygen species and nitric oxide in chemical and biological model systems. Journal of Agricultural and Food Chemistry 51: 5271–77. DOI: https://doi.org/10.1021/jf034466n
Jayaraman R, Yadavalli C, Singh V and Khanum F. 2019. Phenolic compounds and antioxidant activities in dehusked and polished pigmented rice genotypes. Oryza-An International Journal on Rice 56: 263–84. DOI: https://doi.org/10.35709/ory.2019.56.3.3
Lee J H. 2010. Identification and quantification of anthocyanins from the grains of black rice (Oryza sativa L.) genotypes. Food Science and Biotechnology 19: 391–97. DOI: https://doi.org/10.1007/s10068-010-0055-5
Mackon E, Jeazet Dongho Epse Mackon G C, Ma Y, Kashif M H, Ali N, Usman B and Liu P. 2021. Recent insights into anthocyanin pigmentation, synthesis, trafficking, and regulatory mechanisms in rice (Oryza sativa L.) caryopsis. Biomolecules 11(394): 1–25. DOI: https://doi.org/10.3390/biom11030394
Mohan B H, Malleshi N G and Koseki T. 2010. Physico-chemical characteristics and non-starch polysaccharide contents of Indica and Japonica brown rice and their malts. LWT-Food Science and Technology 43: 784–91. DOI: https://doi.org/10.1016/j.lwt.2010.01.002
Mudoi T and Das P. 2019. A study on phytochemicals and mineral content of indigenous red rice of Assam, India. International Journal of Current Microbiology and Applied Science 8: 1–12. DOI: https://doi.org/10.20546/ijcmas.2019.804.001
Pathak K, Rathi S, Sarma R N and Baishya S. 2016. Assessment of physical, chemical and antioxidant properties of few pigmented glutinous rice grown in Northeast India. Indian Journal of Plant Physiology 21: 287–99. DOI: https://doi.org/10.1007/s40502-016-0237-0
Pengkumsri N, Chaiyasut C, Saenjum C, Sirilun S, Peerajan S, Suwannalert P, Sirisattha S and Sivamaruthi B S. 2015. Physicochemical and antioxidative properties of black, brown and red rice varieties of northern Thailand. Food Science and Technology 35(2): 331–38. DOI: https://doi.org/10.1590/1678-457X.6573
Pereira-Caro G, Watanabe S, Crozier A, Fujimura T, Yokota T and Ashihara H. 2013. Phytochemical profile of a Japanese black–purple rice. Food Chemistry 141: 2821–27. DOI: https://doi.org/10.1016/j.foodchem.2013.05.100
Pradipta S, Ubaidillah M and Siswoyo T A. 2020. Physicochemical, functional and antioxidant properties of pigmented rice. Current Researchin Nutrition and Food Science 8: 837–51. DOI: https://doi.org/10.12944/CRNFSJ.8.3.15
Pramai P and Jiamyangyuen S. 2016. Chemometric classification of pigmented rice genotypes based on antioxidative properties in relation to color. Songklanakarin Journal of Science and Technology 38: 463–72.
Rauf A, Imran M, Izneid T A, Haq I U, Patel S, Pan X, Naz S, Silva A S, Saeed F and Suleria H A R. 2019. Proanthocyanidins: A comprehensive review. Biomedicine and Pharmacotherapy 116: 108999. DOI: https://doi.org/10.1016/j.biopha.2019.108999
Reddy M, Turaidar V, Krupa K N, Ramachandra A, Subhash Bharani S and Dalawai N. 2018. Enhancement of iron and zinc in rice grain through biofortification approach. International Journal of Current Microbiology and Applied Science 7: 628–37.
Saikia S, Dutta H, Saikia D and Mahanta C L. 2012. Quality characterisation and estimation of phytochemicals content and antioxidant capacity of aromatic pigmented and non- pigmented rice genotypes. Food Research International 46: 334–40. DOI: https://doi.org/10.1016/j.foodres.2011.12.021
Samyor D, Deka S C and Das A B. 2016. Phytochemical and antioxidant profile of pigmented and non-pigmented rice cultivars of Arunachal Pradesh, India. International Journal of Food Properties 19: 1104–14. DOI: https://doi.org/10.1080/10942912.2015.1055761
Singleton V L, Orthofer R and Lamuela-Raventos R M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagents. Methods in Enzymology 299: 152–78. DOI: https://doi.org/10.1016/S0076-6879(99)99017-1
Wu S J and Ng L T. 2008. Antioxidant and free radical scavenging activities of wild bitter melon (Momordica charantia Linn. var. abbreviata Ser.) in Taiwan. LWT-Food Science and Technology 41: 323–30. DOI: https://doi.org/10.1016/j.lwt.2007.03.003
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