Antagonistic potential and growth promoting activities of native Trichoderma isolates against Fusarium oxysporum f. sp. ciceri


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Authors

  • DEEPAK KUMARI Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
  • N K YADAV Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
  • KISHOR CHAND KUMHAR Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
  • AJAY KUMAR Krishi Vigyan Kendra, Jhajjar, Haryana
  • PREETI VASHISHT Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
  • GARIMA Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India

https://doi.org/10.56093/ijas.v93i7.137287

Keywords:

Bio-control, Chickpea wilt, Cicer arietinum, F. oxysporum f. sp. ciceri, Trichoderma

Abstract

Chickpea wilt caused by Fusarium oxysporum f. sp. ciceri (Foc) has been considered as a devastating one which appears every year and causes heavy losses in yield. Trichoderma is a potential biocontrol agent against many diseases. Present investigation was carried out during winter (rabi) season 2021 to assess the antagonistic potential and growth promoting activities of native Trichoderma isolates against Foc. Thirteen Trichoderma isolates from chickpea rhizosphere were isolated and evaluated under in vitro conditions for their potentiality to antagonise Foc. The highest and lowest per cent of mycelial growth inhibition observed among these isolates was 88.1% (HST-1) and 62.2% (HCdT), respectively. Seed treatment with native T. asperellum plays an important role by enhancing plant growth parameters. Experimental results indicated that seed treatment with native T. asperellum exhibited significant increase in germination (94.29%), vigour index (2883.60), vigour index mass (117.16), plant height (25.64 cm), root length (4.96 cm), fresh plant weight (10.92 g), dry plant weight (1.24 g) and number of primary and secondary branches as compared to control. Additionally, T. asperellum caused plants to accumulate more lignin and showed an inhibitory effect on the occurrence of chickpea Fusarium wilt disease. Seed treatment with T. asperellum reduced disease incidence to 36% as compared to 96% in control (chickpea+Foc).

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References

Abdul Baki A A and Anderson J D. 1973. Vigour determination in soybean by multiple criteria. Crop Science 13(6): 630–33. DOI: https://doi.org/10.2135/cropsci1973.0011183X001300060013x

Argikar G P. 1970. Pulse crops of India, pp. 54–135. Indian Council of Agricultural Research, New Delhi, India.

Dubey S C, Singh S R and Singh B. 2010. Morphological and pathogenic variability of Indian isolates of Fusarium oxysporum f. sp. ciceris causing chickpea wilt. Archives of Phytopathology and Plant Protection 43(2): 174–90. DOI: https://doi.org/10.1080/03235400802021108

FAOSTAT. 2021. Available online: http://www.fao.org/faostat/ en/#data (accessed on March 11, 2021).

Guo D, Chen F, Inoue K, Blount J W and Dixon R A. 2001. Down regulation of caffeic acid 3-O methyltransferase and caffeoyl CoA 3-O-methyltransferase in transgenic alfalfa: Impacts on lignin structure and implications for the biosynthesis of G and S lignin. Plant Cell 13: 73–88. DOI: https://doi.org/10.1105/tpc.13.1.73

Harman G E, Howell C R, Viterbo A, Chet I and Lorito M. 2004. Trichoderma species-opportunistic, avirulent plant symbionts. Nature reviews microbiology 2(1): 43–56. DOI: https://doi.org/10.1038/nrmicro797

Johnson L F and Curl E A. 1972. Methods for Research on Ecology of Soil-borne Plant Pathogens, p. 247. Burgess Publishing Company, Minneapolis, USA.

Johnson L Z. 1957. Phytopathological notes. Phytopathology 47: 630.

Larkin R P and Fravel D R. 1998. Efficacy of various fungal and bacterial biocontrol organisms for control of Fusarium wilt of tomato. Plant Disease 82(9): 1022–28. DOI: https://doi.org/10.1094/PDIS.1998.82.9.1022

Meher J, Singh S N and Sonkar S S. 2018. Growth promotion of chickpea plant on treatment with native isolates of Trichoderma spp. Journal of Pharmacognosy and Phytochemistry 7(4): 1631–36. DOI: https://doi.org/10.20546/ijcmas.2018.701.025

Mishra R K, Mishra M, Pandey S, Saabale P R and Naimuddin. 2021. Exploring the indigenous Trichoderma strains from pulses rhizosphere and their biocontrol potential against Fusarium oxysporum f. sp. ciceri in chickpea. Indian Phytopathology 75(1): 273–78. DOI: https://doi.org/10.1007/s42360-021-00416-1

Nagamani P, Bhagat S, Biswas M K and Viswanath K. 2020. Antagonistic and plant growth promoting activity of Trichoderma spp. against soil borne diseases of chickpea. Indian Council of Agricultural Research, New Delhi, India.

Patel S and Saraf M. 2017. Biocontrol efficacy of Trichoderma asperellum MSST against tomato wilting by Fusarium oxysporum f. sp. lycopersici. Archives of Phytopathology and Plant Protection 50(5–6): 228–38. DOI: https://doi.org/10.1080/03235408.2017.1287236

Rangaswami G. 1972. Diseases of crop plants in India, pp. 520. Prentice Hall of India Pvt. Ltd., New Delhi.

Singh G, Chen W, Rubiales D, Moore K, Sharma Y R and Gan Y. 2007. Twenty four diseases and their management. Chickpea breeding and management, p. 497. Cromwell Press, Trowbridge, UK. DOI: https://doi.org/10.1079/9781845932138.024

Singh K B. 1997. Chickpea (Cicer arietinum L.). Field crops research 53(1–3): 161–70. DOI: https://doi.org/10.1016/S0378-4290(97)00029-4

Singh P, Kumar A, Singh S N and Kumar S. 2018. Antagonistic variability among the isolates of Trichoderma against Fusarium oxysporum f. sp. ciceri. International Journal of Current Microbiology and Applied Sciences 7(8): 4833–39. DOI: https://doi.org/10.20546/ijcmas.2018.708.509

Singh V, Upadhyay R S, Sarma B K and Singh H B. 2016. Trichoderma asperellum spore dose dependent modulation of plant growth in vegetable crops. Microbiological research 193: 74–86. DOI: https://doi.org/10.1016/j.micres.2016.09.002

Suresh M, Kumar C V and Sudhakar R. 2011. Efficacy of native isolates of Trichoderma sp. against chickpea wilt in southern Telangana zone of Andhra Pradesh. International Journal of Bio-resource and Stress management 2(1): 48–53.

Vincent J M. 1947. Distortion of fungal hyphae in presence of certain inhibitors. Nature 159: 850. DOI: https://doi.org/10.1038/159850b0

Younesi H, Bazgir E, Darvishnia M and Chehri K. 2021. Selection and control efficiency of Trichoderma isolates against Fusarium oxysporum f. sp. ciceris in Iran. Physiological and Molecular Plant Pathology 116: 101731. DOI: https://doi.org/10.1016/j.pmpp.2021.101731

Yusnawan E, Inayati A and Baliadi Y. 2019. Isolation of antagonistic fungi from rhizospheres and its biocontrol activity against different isolates of soil borne fungal pathogens infected legumes. Biodiversitas Journal of Biological Diversity 20(7). DOI: https://doi.org/10.13057/biodiv/d200735

Submitted

2023-06-05

Published

2023-08-08

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Articles

How to Cite

KUMARI, D., YADAV, N. K., KUMHAR, K. C., KUMAR, A., VASHISHT, P., & GARIMA. (2023). Antagonistic potential and growth promoting activities of native Trichoderma isolates against Fusarium oxysporum f. sp. ciceri. The Indian Journal of Agricultural Sciences, 93(7), 780–785. https://doi.org/10.56093/ijas.v93i7.137287
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