Impact of physio-biochemical responses on amelioration of short-term storage and management of post-harvest seed quality of greengram (Vigna radiata)

Abstract views: 116 / PDF downloads: 82


  • B BORUAH Assam Agricultural University, Jorhat, Assam 785 013, India image/svg+xml
  • S D DEKA Assam Agricultural University, Jorhat, Assam 785 013, India image/svg+xml
  • P SHARMA Assam Agricultural University, Jorhat, Assam 785 013, India image/svg+xml
  • S GOGOI Assam Agricultural University, Jorhat, Assam 785 013, India image/svg+xml
  • B SARMAH Assam Agricultural University, Jorhat, Assam 785 013, India image/svg+xml
  • P D NATH Assam Agricultural University, Jorhat, Assam 785 013, India image/svg+xml


α-amylase, Dehydrogenase activity, Emamectin benzoate, HDPE interwoven bags, Lipid peroxidation, Seed storage


The present study was carried out during winter (rabi) and rainy (kharif) seasons of 2021 and 2022 at Assam Agricultural University, Jorhat, Assam to address the significance of maintaining post-harvest seed quality and its management throughout the nation, and to promote the knowledge and create awareness among the farming communities and pulse growers at the national level in terms of the area with high rainfall and high relative humidity. The greengram [Vigna radiata (L.) R. Wilczek] variety (SGC-16) seeds were stored in 4 different types of packaging materials, viz. jute bag, polylined jute bags; HDPE (High Density Polyethylene) interwoven bags; and 700-gauze polythene bags and treated with treatment combination of emamectin benzoate (40 mg/kg) (chemical based); 5 ml/kg neem oil (plant based); 3 g/kg black pepper (recommended practise for the state); and 3 g/kg silica gel (desiccants). With an increase in storage time, a gradual decline in seed quality parameters such as viability, germination, seedling length, seedling dry weight, Seed vigour index-I, Seed vigour index-II, germination index value, field emergence, and chlorophyll content was observed. Variations for biochemical indicators of seed quality were also recorded. The seed germination above Indian Minimum Seed Certification Standard (IMSCS) level was maintained by all treatments up to 120 days of storage. However, emamectin benzoate (40 mg/kg) along with 3 g/kg of silica gel in HDPE interwoven bags found to be best in keeping seed quality standards with lowest lipid peroxidation activity (0.36 g/gm) and highest in seeds stored in jute bag (0.68 g/gm).


Download data is not yet available.


Agarwal V K. 1976. Technique for the detection of seed borne fungi. Seed Research 4: 24–31.

Anonymous. 2022. Directorate of Pulses Development, Vindhyachal Bhawan, Bhopal, Madhya Pradesh.

AOSA. 1983. Seed Vigour Testing Handbook. Contribution No: 32 to Handbook on Seed Testing. Association of Official Seed Analysis.

Aron D. 1949. Copper enzymes isolated chloroplasts, polyphenoloxidase in Beta vulgaris. Plant Physiology 24: 1–15.

Copeland L O and Mc Donald M B. 1985. Principle of Seed Science and Technology. Springer Sciences and Business Media.

Gomez A K and Gomez A A. 1984. Statistical Procedures for Agricultural Research. John Wiley and Sons.

Heath R L and Packer L. 1968. Photo peroxidation in isolated chloroplast I. Kinetics and stoichiometry of fatty acid peroxidation. Archieves of Biochemistry and Biophysics 125: 189–98.

Hou D, Yousaf L, Xue Y, Hu J, Wu J, Hu X, Feng N and Shen Q. 2019. Mungbean (Vigna radiata L.): Bioactive polyphenols, polysaccharides, peptides and health benefits. Nutrients 11(6): 1238.

ISTA. 2004. Seed Sampling, 2nd edn. International Seed Testing Association, Zurich. ISTA. 2019. ISTA Rules for Seed Testing. 14: 6–17.

Khan A A and Faust A M. 1967. Effect of growth retardants on α amylase production in germinating barley seed. Plant Physiology 20: 673–81.

Khatun A, Kabir G and Bhuiyan M A H. 2009. Effect of harvesting stages on seed quality of lentil (Lens culinaris L.) during storage. Bangladesh Journal of Agricultural Research 34(4): 565–76.

Kittock D L and Law A G. 1968. Relationship of seedling vigour to respiration and tetrazolium reduction in germinating wheat seeds. Journal of Agronomy 60: 268–88.

Lakon G. 1942. Topographischer nachweis der keimfahigkeit der getreidefruchte durch tetrazoliumsalze. Berichte der Deutschen Botanischen Gesellscha 60: 299–305.

Lin Y X, Xu H J, Yin G K, Zhou Y C, Lu X X and Xin X. 2022. Dynamic changes in membrane lipid metabolism and antioxidant defense during soybean (Glycine max L. Merr.) seed aging. Frontiers in Plant Science 13: 908–49.

Marichamy M S, Jyothsna S, Harini V, Devi B, Rajapriya B, Rajalakshmi A and Ahaljith R. 2020. Studies on effect of different packaging materials and storage temperature on physiological loss in weight of amaranthus (Amaranthus viridis). International Journal of Current Microbiology and Applied Science 9: 720–31.

Minhas A. 2022. Pulses in India, Statistics and Facts. Statista Monira U S, Amin M H A, Aktar M M and Mamun M A A. 2012. Effect of containers on seed quality of storage soybean seed. Bangladesh Research Publications Journal 7(4): 421–27.

Nie L, Song S, Yin Q, Zhao T, Liu H, He A and Wang W. 2022. Enhancement in seed priming-induced starch degradation of rice seed under chilling stress via ga-mediated α-amylase expression. Rice 15: 19

Odokonyero K, Adair Gallo J R and Mishra H. 2021. Nature-inspired wax-coated jute bags for reducing post-harvest storage losses. Scientific Reports 11: 153–54.

Onder S, Dayan E, Karakurt Y and Tonguc M. 2022. Changes in germination, antioxidant enzyme activities and biochemical contents of safflower (Carthamus tinctorius L.) under different salinity levels. University of Arts and Science Journal of Science 17(1): 185–94.

Sanjay S, Odedra K N and Jadeja B A. 2023. Study of some physiological and biochemical changes of Greengram seeds in three different storage containers. Journal of Pharmacognosy and Phytochemistry 12: 690–97.

Satasiya R M, Antala D K, Sojitra M A, Kothiya A V and Chauhan P M. 2021. Effect of packaging on storage behaviour of chickpea grain. Journal of Pharmacognosy and Phytochemistry 10: 1575–584.

Xing M, Long Y, Wang Q, Tian X, Fan S, Zhang C and Huang W. 2023. Physiological alterations and non-destructive test methods of crop seed vigour: A comprehensive review. Agriculture 13: 527.

Zhang Q, Pritchard J, Mieog J, Byrne K, Colgrave M L, Wang J R and Ral J P F. 2022. Over-expression of a wheat late maturity α-amylase type 1 impact on starch properties during grain development and germination. Frontiers in Plant Science 13: 811728.









How to Cite

BORUAH, B. ., DEKA, S. D. ., SHARMA, P. ., GOGOI, S. ., SARMAH, B. ., & NATH, P. D. . (2024). Impact of physio-biochemical responses on amelioration of short-term storage and management of post-harvest seed quality of greengram (Vigna radiata). The Indian Journal of Agricultural Sciences, 94(6), 626–631.