In vitro propagation of Pyrus communis cv. Bartlett

M A PADDER; M A MIR; K M BHATT; Z A RATHER; G HUSSAIN

doi:10.56093/ijas.v82i3.15942

Authors

M A PADDER Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar 191 121
M A MIR Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar 191 121
K M BHATT Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar 191 121
Z A RATHER Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar 191 121
G HUSSAIN Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar 191 121

https://doi.org/10.56093/ijas.v82i3.15942

Keywords:

Bartlett, Forcing, In vitro propagation, Pyrus communis

Abstract

Shoot tip explants derived from forced and unforced explants were subjected to different sterilization regimes. Satisfactory culture asepsis (61.60%) and survival (45.00%) was obtained with 0.1% mercuric chloride when forced explants were used. MS medium supplemented with BAP+IBA (1.5+0.01mg/l) resulted in maximum explant establishment (46.66%). Maximum proliferating cultures (52.66%) and shoot number/explant (8.73) was recorded in MS medium supplemented with BAP+IBA (2.00+0.01mg/l). Microcuttings (10-15mm) from the proliferated cultures were inoculated in two media supplemented with different IBA concentrations for rooting. MS medium fortified with IBA (1.0 mg/l) resulted in maximum rooting (46.66%), root number/shoot (4.26) and root length (36.50 mm).

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