The protective role of methyl jasmonates (MeJA) in capsicum (Capsicum annuum var. conoide) under drought stress: Growth, photosynthetic, and biochemical responses

SAJID ALI  SHAH; SUNNY SINGH  CHIB; SAIMA  ASLAM

doi:10.56093/ijas.v96i02.167779

Authors

SAJID ALI SHAH Baba Ghulam Shah Badshah University, Rajouri, Jammu and Kashmir 185 234, India
SUNNY SINGH CHIB Baba Ghulam Shah Badshah University, Rajouri, Jammu and Kashmir 185 234, India
SAIMA ASLAM Baba Ghulam Shah Badshah University, Rajouri, Jammu and Kashmir 185 234, India

https://doi.org/10.56093/ijas.v96i02.167779

Keywords:

Antioxidant defense, Drought resilience, Foliar spray, Stomatal regulation, Water-deficit conditions

Abstract

Drought stress severely limits crop productivity and negatively affects the growth and physiology of capsicum (Capsicum annuum var. conoide). The present study was carried out during 2023–2024 at the Department of Biotechnology, Baba Ghulam Shah Badshah University, Rajouri, Jammu and Kashmir to evaluate the role of foliar-applied methyl jasmonate (MeJA, 25 and 50 μM) in improving drought tolerance. Drought significantly reduced growth, biomass, yield traits, photosynthetic pigments, and induced oxidative damage (H2O2 and MDA accumulation). MeJA application, particularly at 25 μM, alleviated these effects by enhancing growth, restoring pigment content, boosting antioxidant enzymes (CAT and SOD), improving protein levels, regulating elemental balance (C, Ca, K), and partially recovering stomatal traits. Statistical analysis confirmed the significance of these improvements. Overall, low-dose MeJA foliar spray effectively enhances drought resilience in C. annuum var. conoide under water-deficit conditions.

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References

Aebi H. 1984. Catalase in vitro. (In) Methods in Enzymology, Vol. 105, pp. 121–26. Academic Press.

Akbar M U, Aqeel M, Shah M S, Jeelani G, Iqbal N, Latif A and Noman A. 2023. Molecular regulation of antioxidants and secondary metabolites act in conjunction to defend plants against pathogenic infection. South African Journal of Botany 161: 247–57.

Arnon D I. 1949. Copper enzymes in isolated chloroplasts: Polyphenoloxidase in Beta vulgaris. Plant Physiology 24(1): 1–15.

Ballesteros G I, Newsham K K, Acuna-Rodríguez I S, Atala C, Torres-Díaz C and Molina-Montenegro M A . 2 0 2 4 . E x t r e m e environments as sources of fungal endophytes mitigating climate change impacts on crops in Mediterranean-type ecosystems. Plants, People, Planet 6(1): 148–61.

Dhindsa R S and Matowe W. 1981. Drought tolerance in two mosses: Correlated with enzymatic defence against lipid peroxidation. Journal of Experimental Botany 32(1): 79–91.

Ghoname A A, AbdelMotlb N A, Abdel-Al F S, Abu El-Azm N A, Abd Elhady S A, Merah O and Abdelhamid M T. 2023. Brassinosteroids or proline can alleviate yield inhibition under salt stress via modulating physio-biochemical activities and antioxidant systems in snap bean. Journal of Horticultural Science and Biotechnology 98(4): 526–39.

Grossmann, Klaus, Jacek Kwiatkowski and Stefan Tresch. 2001. Auxin herbicides induce H2O2 overproduction and tissue damage in cleavers (Galium aparine L.). Journal of Experimental Botany 52(362): 1811–16.

Grozeva S and Ganeva D. 2024. Effects of the drought-induced stress on the flowering and fruit set stages in tomato collection. Agricultural Science and Technology 16(41): 29–38.

Guihua Z, Feifei G, Rui X, Bo L, Zhuowu W, Qun Z and Zhenwen Z. 2024. The ameliorative effects of exogenous MeJA on grapevines under drought stress: Reactive oxygen species, carbon and nitrogen metabolism. Scientia Horticulturae 335: 113354.

Hatice Y C, Semsettin K and Hakan S. 2023. Effect of drought and UV-B stress on stoma characteristics in two maple species. Journal of Engineering Sciences 9(1): 1–9.

Huang Y, Wang H, Zhu Y, Huang X, Li S, Wu X and Wu Y. 2022. THP9 enhances seed protein content and nitrogen-use efficiency in maize. Nature 612(7939): 292–300.

Kesawat M S, Satheesh N, Kherawat B S, Kumar A, Kim H U, Chung S M and Kumar M. 2023. Regulation of reactive oxygen species during salt stress in plants and their crosstalk with other signaling molecules– Current perspectives and future directions. Plants 12(4): 864.

Khan V, Mubashshir M, Umar S and Iqbal N. 2024. MeJA and Pseudomonas fluorescens synergistically boost antioxidative defense, secondary metabolites and osmolyte production to enhance drought resilience in mustard. Journal of Plant Growth Regulation 44: 198–216.

Krishna M S R. 2018. Arbuscular mycorrhizal symbiosis alters morphological and biochemical indices in hot pepper (Capsicum annuum L.) under drought stress. International Journal of Green Pharmacy 12(2).

Lowry O H, Rosebrough N J, Farr A L and Randall R J. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193: 265–75.

Ma C, Tao Z, Yang X, Xiao R and Jia K. 2025. Transcriptome and physiological characterization reveal the effects of exogenous MeJA on turnips under salt stress. Horticulturae 11(2): 197.

Molla A E, Andualem A M, Ayana M T and Zeru M A. 2023. Effects of drought stress on growth, physiological and biochemical parameters of two Ethiopian red pepper (Capsicum annuum L.) cultivars. Journal of Applied Horticulture 25(1): 32–38.

Muneer M A, Afridi M S, Saddique M A B, Chen X, Yan X, Farooq I and Wu L. 2024. Nutrient stress signals: Elucidating morphological, physiological and molecular responses of fruit trees to macronutrients deficiency and their management strategies. Scientia Horticulturae 329: 112985.

Ndiaye A, Diallo A, Fall N C, Diouf R D, Diouf D and Kane N A. 2022. Transcriptomic analysis of MeJA treatment reveals gene networks involved in drought tolerance in pearl millet. Science Reports 12(1): 10–432.

Naoghare P K, Kwon H T and Song J M. 2009. Development of a photosensitive, high-throughput chip-based superoxide dismutase (SOD) assay to explore the radioprotective activity of herbal plants. Biosensors and Bioelectronics 24(12): 3587–93. Nguyen T H, Silva-Alvim F A, Hills A and Blatt M R. 2023. OnGuard3e: A predictive, ecophysiology-ready tool for gas exchange and photosynthesis research. Journal of Experimental Botany 11: 3644–58.

Nimrah T, Naz R, Yasmin H, Nosheen A, Keyani R, Sajjad M, Hassan M N and Roberts T H. 2020. Combined seed and foliar pre-treatments with exogenous MeJA and salicylic acid mitigate drought-induced stress in maize. PLoS ONE 15(5): e0232269.

Noor M, Fan J, Kaleem M, Akhtar M T, Jin S, Nazir U and Yan X. 2024. Assessment of the changes in growth, photosynthetic traits and gene expression in Cynodon dactylon against drought stress. BMC Plant Biology 24(1): 235.

Okuda M, Li K, Beard M R, Showalter L A, Scholle F, Lemon S M and Weinman S A. 2002. Mitochondrial injury, oxidative stress and antioxidant gene expression are induced by hepatitis C virus core protein. Gastroenterology 122(2): 366–75.

Rehman F, Saeed A, Yaseen M, Shakeel A, Ziaf K, Munir H and Riaz A. 2019. Genetic evaluation and characterisation using cluster heat map to assess NaCl tolerance in tomato germplasm at the seedling stage. Chilean Journal of Agricultural Research 1: 56–65.

Rocha R P, Melo E C and Radünz L L. 2011. Influence of drying process on the quality of medicinal plants: A review. Journal of Medicinal Plants Research 5(33): 7076–84.

Sachdev S, Ansari S A, Ansari M I, Fujita M and Hasanuzzaman M. 2021. Abiotic stress and reactive oxygen species: Generation, signaling and defense mechanisms. Antioxidants 10(2): 277.

Samanta S and Roychoudhury A. 2025. Molecular crosstalk of jasmonate with major phytohormones and plant growth regulators during diverse stress responses. Journal of Plant Growth Regulation 44(1): 62–88.

Shah S A, Arshad M and Aslam S. 2025. Comprehensive review on the role of exogenous phytohormones in enhancing temperature stress tolerance in plants. Journal of Crop Health 77: 136.

Soto-Hernandez M, Aguirre-Hernandez E, Palma-Tenango M and Surguchov A. 2024. Phytochemicals in Agriculture and Food, Vol. 53. IntechOpen. http://dx.doi.org/10.5772/ intechopen.104363

Tayyab N, Naz R, Yasmin H, Nosheen A, Keyani R, Sajjad M, Hassan M N and Roberts T H. 2020. Combined seed and foliar pre-treatments with exogenous MeJA and salicylic acid mitigate drought-induced stress in maize. PLoS ONE 5: 0232269.

Wang C, Zhang J, Li J, Xie J and Chai Q. 2024. Exogenous MeJA regulates endogenous hormone synthesis of soilless cultivated Chinese chive to promote growth physiology and photosynthesis. Scientia Horticulturae 327: 112861.

Wubetie A, Wassie A, Andualem A, Abiyu E, Molla Z, Tarekegn M, Mersha W, Aragaw M and Misganaw T. 2023. Growth, physiological and biochemical responses of Ethiopian red pepper (Capsicum annuum L.) cultivars to drought stress. Scientific World Journal 2023: 4374318.

Xiao H, Hu Y, Zhang G, Jiang Y, Chen X and Yu D. 2018. Jasmonate negatively regulates stomatal development in arabidopsis cotyledons. Plant Physiology 176(4): 2871–85.

Yan S, Luo S, Dong S, Zhang T, Sun J, Wang N and Shen Y. 2014. Heterotrimeric G-proteins involved in the MeJA-regulated ion flux and stomatal closure in Arabidopsis thaliana. Functional Plant Biology 42(2): 126–35.

Yaqin J, Jun L C, Ying S, Qian Z and Chongli X. 2022. Effects of drought stress on growth, photosynthesis and ultrastructure of mesophyll cell of Ulmus szechuanica Fang seedlings. Bangladesh Journal of Botany 51(3): 461–68.

Yavasli D D and Erlat E. 2023. Climate model projections of aridity patterns in Turkiye: A comprehensive analysis using CMIP6 models and three aridity indices. International Journal of Climatology 43(13): 6207–24.

Yu T Y, Xu C X, Li W J and Wang B. 2022. Peptides/receptors signaling during plant fertilization. Frontiers in Plant Science 13: 1090836.

Zaid A, Mohammad F and Siddique K H. 2022. Salicylic acid priming regulates stomatal conductance, trichome density and improves cadmium stress tolerance in Mentha arvensis L. Frontiers in Plant Science 13: 895427.