Morpho-Molecular Characterization of different isolates of Colletotrichum spp. and Screening of local germplasms of Manipur, Eastern Himalayan Region  against the Anthracnose Disease

Brajesh Heisnam; Sumitra Phurailatpam; Kaviya R; Baby Wangkhem; Rupert Anand Yumlembam; Pukhram Bhumita; Vijay Kumar

doi:10.56093/ijas.v96i10.174346

Authors

Brajesh Heisnam
Sumitra Phurailatpam
Kaviya R
Baby Wangkhem
Rupert Anand Yumlembam
Pukhram Bhumita
Vijay Kumar

https://doi.org/10.56093/ijas.v96i10.174346

Abstract

A comprehensive survey was conducted during the year 2022 to assess the occurrence and variability of chilli anthracnose across five major chilli-growing districts of Manipur. A total of 35 fields were evaluated, with disease incidence ranging from 26% to 41%, and the highest recorded in Imphal West. Among locations, Kwasiphai exhibited the highest incidence (74%), while Sekmaijin showed the lowest (16%). The seven isolates of Colletotrichum spp. were collected from surveyed districts and characterized morphologically and molecularly. Considerable variability was observed in colony traits, growth, sporulation, and conidial morphology. ITS-based phylogenetic analysis revealed six isolates as Colletotrichum truncatum and only one as C. brevisporum. Pathogenicity testing demonstrated significant virulence variation, with isolate C-05 identified as the most aggressive (80.25% PDI). The eight local chilli germplasm were evaluated for resistance using in vitro inoculation (with and without injury) and under polyhouse conditions. Without injury, two landraces (T6 and T8) exhibited immunity, while all genotypes became highly susceptible after injury. Under polyhouse conditions, T8 was resistant, five were moderately resistant, and two were susceptible. The study highlights high disease prevalence, substantial pathogen diversity, and identifies promising landraces for resistance breeding, particularly Morok Amubi (T8), offering valuable insights for disease management and cultivar development.

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Author Biography

Vijay Kumar

Dr. Vijay Kumar, Assistant Professor (Plant Protection), Multi Technology Testing Centre and Vocational Training Centre, Lembucherra, Tripura.

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