Micropropagation of banana (Musa acuminata) through proliferation of axillary shoots
Abstract views: 144 / PDF downloads: 133
https://doi.org/10.56093/ijas.v82i5.17811
Keywords:
Banana, Micropropagation, Multiplication rate, Sucker, Shoot-tipAbstract
An efficient and reproducible micropropagation protocol was developed for mass multiplication of the elite banana (Musa acuminata L.) cultivar Grand Naine through axillary shoot proliferation. In vitro multiplication of banana was studied. Conventional propagation through suckers has disadvantages like low multiplication rate (5 to 10 in number depending upon variety), lack of uniform plant size and transmission of pathogens from one generation to the next generation. Application of micropropagation technique for large scale production of elite clones is an effective and superior alternative to propagation through conventional cuttings of Musa spp. Shoot tips were cultured on MS medium supplemented with 5.0 mg/l BAP. Observations were recorded at the end of each subculture, after three weeks of shifting on a new medium. It was noted that suckers of same genotype exhibited different shoot multiplication rates under in vitro conditions. On an average, upto 175.57 shoots were produced from each shoot tip after seven subculturings.
Downloads
References
Abdullah K, Khan I A, Siddiqui S H, Ahmed M and Siddiqui K A. 1997. In vitro culture of indigenous and exotic banana clones for maximum multiplication. Pakistan Journal of Botany 29: 143– 50.
Alloufa M A I, Macedo C E C, Barroso P A V, Barbalho A D and Oliveira C H B. 2002. Evaluation of the use of inflorescences of banana plant variety ‘Pacovan’ (Musa spp.) as explants source for the culture in vitro in the presence of different antioxidant agents. Ciencia e Agrotecnologia 26:1092–6.
Alvard D, Cote F and Teisson C. 1993. Comparison of methods of liquid medium culture for banana micropropagation. Plant Cell, Tissue and Organ Culture 32: 55–60.
Cote F D, Alvard R, Domergue L, Mastache N and Teisson C. 1990. Micropropagation in vitro du bananier. Fruits 45: 112–8.
Dale J L. 1987. Banana Bunchy Top: An Economically Important Tropical Plant Virus Disease. Advances in Virus Research 33: 301–25.
Darvari F M, Sariah M, Puad M P and Maziahl M. 2010. Micropropagation of some Malaysian banana and plantain (Musa spp.) cultivars using male flowers. African Journal of Biotechnology 9: 2360–6.
Doreswamy R, Rao N K S and Chacko E K. 1983. Tissue-culture propagation of banana. Scientia Horticulturae 18: 247–52.
Drew R A and Smith M K. 1990. Field evaluation of tissue cultured bananas in southeastern Queensland. Australian Journal of Experimental Agriculture 30: 569–74.
Ferreira S A, Trujillo E E and Ogata D Y. 1997. Banana bunchy top virus. Plant Disease 12: 1–4.
Israeli Y, Lahav E and Reuveni O. 1995. In vitro culture of bananas. Fruits 43: 219–23.
Karan M, Hardy R M and Dale J L 1994. Evidence for two groups of banana bunchy top virus isolates. Journal of General Virology 75: 3541–6.
Ma S S and Shii C T. 1972. In vitro formation of adventitious buds in banana shoot apex following decapitation. Journal of Chinese Society of Horticultural Sciences 18: 135–42.
Mendes B M J, Filippi S B, Demetrio C G B and Rodriguez A P M. 1999. A statistical approach to study the dynamics of micropropagation rates, using banana (Musa spp.) as an example. Plant Cell Reports 18: 967–71.
Muhammad A, Hussain I, Saqlan Naqvi S M and Rashid H. 2004. Banana plantlet production through tissue culture. Pakistan Journal of Botany 36: 617–20.
Murashige T and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473–97.
Novak F J, Afza R, Duren M V, Dallos M P, Conger B V and Xiaolang T. 1989. Somatic embryogenesis and plant regeneration in suspension cultures of dessert (AA and AAA) and cooking (ABB) bananas (Musa spp.). Bio-Technology 7: 154–59.
Ranasingh N. 2007. Field diagnosis and management of banana bunchy top disease. Orissa Review: 78–80.
Resmi L and Nair A S. 2007. Plantlet production from male inflorescence tips of Musa acuminata cultivars from South India. Plant Cell, Tissue and Organ Culture 88: 333–8.
Vishnoi R, Raj S K and Prasad V 2009. Molecular characterization of an Indian isolate of Banana bunchy top virus based on six genomic DNA components. Virus Genes 38: 334–44.
Vuylsteke D. 1998. Shoot-tip culture for the propagation, conservation and distribution of Musa germplasm, 82 pp. IITA, Ibadan, Nigeria.
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2014 The Indian Journal of Agricultural Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Agricultural Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.