A protocol for efficient callus induction from hypocotyls explant in chickpea (Cicer arietinum)
Abstract views: 167 / PDF downloads: 45
https://doi.org/10.56093/ijas.v84i1.37179
Keywords:
Callus, Chickpea, HypocotylsAbstract
Callus induction in any crop is a pre-requisite for its utilization in tissue culture based techniques like somaclonal variation, screening of calli against biotic or abiotic stress, regeneration of plant and transgenic plant development. In present investigation an attempt was made to develop a protocol for efficient callus induction from hypocotyl explants in chickpea crop. Among different combinations of growth regulators- auxin alone and in combination with cytokinin used for callus induction, MS basal medium supplemented with IAA (2.5 mg/L) + BAP (0.5 mg/L), 2, 4-D (2 mg/L) and NAA (3 mg/L) + BAP (3 mg/L) were induced significantly higher callus induction (100%) in all genotypes of chickpea E100 Y (M), Gaurav and Pb-7) and (L-550) from hypocotyls explant.
Downloads
References
Brown D C W and Thorpe T A. 1995. Crop improvement through tissue culture. World Journal of Microbiology and Biotechnology 11: 409–15. DOI: https://doi.org/10.1007/BF00364616
Gawande A S, Ghive D V, Ghorade R B, Barbade N P and Pote S R. 2007. Studies on regeneration potential of callus in chickpea cv. ICCV-2. Asian Journal Bio-Science 2: 63–5.
Haware M P. 1998. Diseases of chickpea, The Pathology of Food and Pasture Legume, pp 473-516. Allen D J and Lenne J M (Eds). CAB International, Willingford, UK and ICRISAT, Patancheru, India.
Huda S, Siddique M A, Khatun N, Rahman, M H and Morshed M. 2003. Regeneration of shoot from cotyledon derived callus of chickpea (Cicer arietinum L.). Pakistan Journal of Biological Sciences 6(15): 1 310–13. DOI: https://doi.org/10.3923/pjbs.2003.1310.1313
Jain S M. 2001. Tissue culture-derived variation in crop improvement. Euphytica, 118(2): 153–66. DOI: https://doi.org/10.1023/A:1004124519479
Mallikarjana N and Muehlbauer F J. 2011. Chickpea hybridization using in vitro techniques. Methods in Molecular Biology 710: 93–105. DOI: https://doi.org/10.1007/978-1-61737-988-8_8
Murashige T and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum 15: 473–97. DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Naz S, Ali A and Iqbal J. 2008. Phenolic content in vitro cultures of chick pea (Cicer arietinum L.) during callogenesis and organogenesis. Pakistan Journal of Botony 46(6): 2 525–39.
Pande S, Ramgopal D, Kishore G K, Mallikarjuna N, Sharma M, Pathak M and Rao J N. 2006. Evaluation of wild Cicer species for resistance to ascochyta blight and botrytis gray mold in controlled environment at ICRISAT, Patencheru, India. ICPN 13: 25–7.
Rao B G and Chopra V L. 1987. Genotypic and explants differences in callus initiation and maintenance in chickpea. International Chickpea and Pigeonpea Newsletter 17: 10–12.
Robertson L D, Ocampo B and Singh K B. 1997. Morphological variation in wild annual Cicer species in comparison to the cultigens. Euphytica 95: 309–19. DOI: https://doi.org/10.1023/A:1003004516921
Singh C, Singh P and Singh R. 2003. Chickpea (Cicer arietinum L.), Modern Techniques of Raising Field Crops, pp 195–208. Singh C, Singh P and Singh R (Eds). Oxford & IBH Pub. Co. Pvt Ltd, New Delhi.
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2014 The Indian Journal of Agricultural Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Agricultural Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.