Polyamine-induced somatic embryogenesis and plantlet regeneration in vitro from plumular explants of dwarf cultivars of coconut (Cocos nucifera)

RAJESH M K; RADHA E; SAJINI K K; ANITHA KARUN

doi:10.56093/ijas.v84i4.39473

Authors

RAJESH M K Central Plantation Crops Research Institute, Kasaragod, Kerala 671 124
RADHA E Indian Institute of Spices Research, Experimental Farm, Peruvannamuzhi P O Kozhikode, Kerala 673 528
SAJINI K K Central Plantation Crops Research Institute, Kasaragod, Kerala 671 124
ANITHA KARUN Central Plantation Crops Research Institute, Kasaragod, Kerala 671 124

https://doi.org/10.56093/ijas.v84i4.39473

Keywords:

Coconut, Plumule culture, Polyamines, Regeneration, Somatic embryogenesis

Abstract

Regeneration of complete plantlets via organogenesis and somatic embryogenesis was achieved from plumular tissues of two dwarf cultivars of coconut, viz. Chowghat Green Dwarf (CGD) and Malayan Yellow Dwarf (MYD). Significant differences were noticed between varieties for the formation of embryogenic calli. There were also significant differences for interaction between variety and regeneration medium with respect to formation of somatic embryos. Well developed plantlets were acclimatized under green house conditions and then successfully established in the field. The development of an efficient method of cloning coconut using plumular explants (with more rapid development of calli and somatic embryos and greater frequencies of plant regeneration compared with calli from inflorescence or leaf tissues) offers a potential for the development of a long-term in vitro means of conserving significant coconut germplasm by cryopreservation of plumular explants. Mass multiplication of elite palms selected on the basis of resistance to root (wilt) diseases of coconut is possible using plumular explants. Plumule cultures can be used for rapid multiplication of proven coconut hybrids. This study can also form a model for future regeneration studies from adult tissues of coconut.

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References

Bais H P and Ravishankar G A. 2002. Role of polyamines in the ontogeny of plants and their biotechnological applications. Plant Cell Tissue Organ Culture 69: 1–34.

De-la-Pena C, Galaz-Avalos R M and Loyola-vargas V M. 2008. Possible role of light and polyamines in the onset of somatic embryogenesis of Coffea canephora. Molecular Biotechnology 39: 215–24. DOI: https://doi.org/10.1007/s12033-008-9037-8

Eeuwens C J. 1976. Mineral requirements for growth and callus initiation of tissue explants excised from mature coconut palms (Cocos nucifera L.) and cultured in vitro. Physiologia Plantarum 36: 23–8. DOI: https://doi.org/10.1111/j.1399-3054.1976.tb05022.x

Hornung R, Domas R and Lynch P T. 2001. Cryopreservation of plumular explants of coconut (Cocos nucifera L.) to support programmes for mass clonal propogation through somatic embryogenesis. Cryo Letters 22: 211–20.

Hou M H, Lin S B, Yuann J M, Lin W C, Wang AH and Kan L S. 2001. Effects of polyamines on the thermal stability and formation kinetics of DNA duplexes with abnormal structure. Nucleic Acids Research 29: 5 121–8. DOI: https://doi.org/10.1093/nar/29.24.5121

Minocha R, Minocha S C and Long S. 2004. Polyamines and their biosynthetic enzymes during somatic embryo development in red spruce (Picea rubens Sarg.). In Vitro Cellular and Developmental Biology- Plant 40: 572–80. DOI: https://doi.org/10.1079/IVP2004569

Nair M K, Anitha Karun and Rajesh M K. 1999. Research potentialities of coconut biotechnology. AgBiotechNet 1: 1-7.

Rajesh M K, Radha E, Anitha Karun and Parthasarathy V A. 2003. Plant regeneration from embryo-derived callus of oil palm- the effect of exogenous polyamines. Plant Cell Tissue Organ Culture 75: 41–7. DOI: https://doi.org/10.1023/A:1024679910085

Smith TA. 1985. Polyamines. Annual Review of Plant Physiology 36: 117–43. DOI: https://doi.org/10.1146/annurev.pp.36.060185.001001

Snedecor G W and Cochran R W. 1975. Statistical methods, pp 1–593. Oxford and IBH Publishing Co, New Delhi.

Takahashi T and Kakehi J I. 2010. Polyamines: ubiquitous polycations with unique roles in growth and stress responses. Annals of Botany 105: 1–6. DOI: https://doi.org/10.1093/aob/mcp259

Wu X B, Wang J, Liu J H and Deng X X. 2009. Involvement of polyamine biosynthesis in somatic embryogenesis of Valencia sweet orange (Citrus sinensis) induced by glycerol. Journal of Plant Physiology 166: 52–62. DOI: https://doi.org/10.1016/j.jplph.2008.02.005