Prediction of faba bean (Vicia faba) seed viability through single seed conductivity test

Abstract

Electrical conductivity of seed leachates is a well developed test to assess the quality of seeds. Membrane integrity and incidence of dead tissue are both influenced by the two major factors determining seed vigour in grain legumes mainly seed ageing and the incidence of imbibition damage. The conductivity of individual seed is not as well known as the conductivity of a seed-lot for analyzing the extent of deterioration of seed. The present investigation was carried out to examine the relationship between conductivity of individual faba bean (Vicia faba L) seeds of three cultivars and their germination. Seed lots of different vigour were obtained through controlled ageing for varying periods. After measuring the leachate conductivity, seeds were allowed to germinate. The conductivity of seeds which were scored as abnormal or dead was predominantly greater than those which produced normal seedlings. The usefulness of this nondestructive method for assessment and prediction of seed viability is discussed.