Analysis of relative nuclear DNA content in carnation (Dianthus caryophyllus) accessions reveals ploidy levels by flow cytometry

H ANSAR; M V DHANANAJAYA; AMREEN TAJ; B FAKRUDIN; A REKHA; RAJIV KUMAR; G K HALESH

doi:10.56093/ijas.v86i11.62930

Authors

H ANSAR ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka 560 089
M V DHANANAJAYA ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka 560 089
AMREEN TAJ ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka 560 089
B FAKRUDIN ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka 560 089
A REKHA ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka 560 089
RAJIV KUMAR ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka 560 089
G K HALESH ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru, Karnataka 560 089

https://doi.org/10.56093/ijas.v86i11.62930

Keywords:

Carnation accessions, Chromosome counting, Flow cytometry, Ploidy level

Abstract

Carnation (Dianthus caryophyllus L.) is one of the fifth most important ornamental species worldwide. Many desirable plant characteristics such as big size flower, adaptation to stress, and intra or interspecific hybridization capability are dependent on plant ploidy level. We optimized a quick flow cytometry method for DNA content determination in carnation accession samples that allowed a systematic evaluation of ploidy levels. To verify the actual ploidy levels, we counted chromosome numbers in the root tips of representative cultivar for each ploidy level. The relative nuclear DNA content was distributed into four kinds of discontinuous groups: 1.32 to 1.95 pg (group 1), 2.03 to 2.72 pg (group 2), 2.98 to 4.65 pg (group 3) and 5.33 pg (group 4) which might correspond to the following ploidy levels; diploid, triploid, tetraploid and hexaploid. The results showed that out of 60 carnation accessions, 33 were diploid, 5 were triploid, 21 were tetraploid and 1 was hexaploid.

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