Standardization of Indian cultivars of Brassica spp. for characterization of Xanthomonas campestris pv. campestris races causing black rot disease of crucifer crops

PRIYANKA SINGH RATHAUR; DINESH SINGH; RICHA RAGHUWANSHI

doi:10.56093/ijas.v86i12.65394

Authors

PRIYANKA SINGH RATHAUR Banaras Hindu University, Varanasi, Uttar Pradesh
DINESH SINGH IARI New Delhi 110 012
RICHA RAGHUWANSHI Banaras Hindu University, Varanasi, Uttar Pradesh 221 005.

https://doi.org/10.56093/ijas.v86i12.65394

Keywords:

Brassica spp, Differentials, Pathogenic variability, Races, Resistance

Abstract

Xanthomonas campestris pv. Campestris (Xcc) causing black rot disease of crucifer crops, is a serious worldwide problem resulting in >50% loss under environmental conditions favorable to the pathogen. For the characterization of Xanthomonas campestris pv. campestris races to standardize the Indian cultivars of Brassica spp. total of 184 representatives lines of the family crucifereae from of five economically important Brassica species, i.e. B. carinata, B. juncea, B. napus, B. oleracea, and B. rapa grown in India were inoculated by 75 strains of Xcc, belonging to three races 1, 4 and 6 in 2012-13 and 2013-14. Disease reaction of these cultivars was compared with the standard differential hosts of crucifer for disease incidence and diseases severity. Indian cultivars of B. juncea (Pusa Bold, Pusa Varuna, Pusa Vijay, Pusa Mustard-21, Pusa Mustard-25, Pusa Sag), B. olerecea var. botrytis (Pusa Sukti), B. olerecea var. capitata (KGMR) and B. rapa (Pusa Swarnima) were at par with the standard differentials of respective Brassica species and thus could be suitable to useas alternative differential hosts for characterization of Xcc races. 20 cultivars of Brassica spp. grown in India were further validated as differential crucifer host to characterize the races of Xcc on the molecular basis using four set of primers, viz. CAPS (ACS2) CAPS (BTPT) and CAPS (NDPK3) and among them, Br 019305 primer amplified at 906 bp in all cultivars, while primer CAPS (ACS2), CAPS (BTPT) and CAPS (NDPK3) primers were amplified multiple bands. Combined phylogenetic UPGMA study at 75% similarity coefficient, 19/20 accessions were divided into three groups and distinguished resistant and susceptible cultivars against black rot disease.

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References

Doullah M, Mohsin G, Ishikawa K, Hori H and Okazaki K. 2011.Construction of a linkage map and QTL analysis for black rot resistance in Brassica oleracea L. International Journal of Natural Sciences.1(1):1–6. DOI: https://doi.org/10.3329/ijns.v1i1.8591

Elvira-Recuenco M, Ellis T H N and Taylor J D. 2000. Inheritance of race non-specific resistance to Pseudomonas syringae pv. pisi derived from Pisum abyssinicum and molecular markers for resistance. (in) Sustainable Control of Pea Bacterial Blight. Approaches For Durble Genetic Resistance and Biocontrol by Endophytic Bacteria. PhD thesis, University of Wageningen, The Netherlands, pp 39–67.

Gomez K A and Gomez A A. 1984. Statistical Procedure for Agriculture Research, 2nd edition. p 104. Coibey-Inter science. Guo H, Dickson M H and Hunter J E. 1991. Brassica napus sources of resistance to black rot in crucifers and inheritance of resistance. Hortscience 26: 1 545–7.

Ignatov A, Kuginuki Y and Hida K. 2000. Distribution and inheritanceof race-specific resistance to X. campestris pv. Campestris in Brassica rapa and B. napus. Journal of Russian Phytopathology Society 1: 89–94.

Kamoun S and Kado CI. 1990. A plant-inducible gene of Xanthomonas campestris pv. campestris encodes an exocellular component required for growth in the host and hypersensitivity on nonhosts. Journal of Bacteriology 172: 5 165–72. DOI: https://doi.org/10.1128/jb.172.9.5165-5172.1990

Kamoun S, KamdarH V, Tola E and Kado C I.1992. Incompatible interactions between crucifers and Xanthomonas campestris involve avascular hypersensitive response: Role of the hrpX locus. Molecular Plant Microbe Interactions 5: 22–23. DOI: https://doi.org/10.1094/MPMI-5-022

Murray H G and Thompson W F 1980. Rapid isolation of high molecular weight DNA. Nucleic Acids Research and 8: 4 321– 5. DOI: https://doi.org/10.1093/nar/8.19.4321

Schaad N W, Jones J B and Lacy G H. 2001. Xanthomonas. Laboratory guide for identification of plant-pathogenic bacteria. American Phytopathological Society Press, St Paul.

Singh D, Dhar S and Yadava D K. 2011. Genetic and pathogenic variability of Indian strains of Xanthomonas campestris pv. Campestris causing black rot disease in crucifers. Current Microbiology 63: 551–60. DOI: https://doi.org/10.1007/s00284-011-0024-0

Singh D, Raghavendra B T, Rathaur P S, Singh H, Raghuwanshi R and Singh R P. 2014. Detection of black rot disease causing

pathogen Xanthomonas campestris pv. Campestris by bio- PCRfrom seeds and plant parts of cole crops. Seed Science and Technology 42: 36–46. DOI: https://doi.org/10.15258/sst.2014.42.1.04

Singh D, Rathaur Priyanka Singh, Singh Ashwariya and Raghuwanshi R. 2015. genetic diversity of Xanthomonas campestris pv. campestris isolated from Brassica crops using RAPD and Rep-PCR. Indian Journal of Agricultural Sciences 85(6): 792–6. DOI: https://doi.org/10.4172/2157-7471.1000317

Soengas P, Hand P, Vicente, J G, Pole J M and Pink, DAC. 2007. Identification of quantitative trait loci for resistance to Xanthomonas campestris pv. campestris in Brassica rapa. Theoretical and Applied Genetics 114: 637–45. DOI: https://doi.org/10.1007/s00122-006-0464-2

Taylor J D, Teverson, D M and Davis J H C. 1996. Sources of resistance to Pseudomonas syringaepv. syringae races in Phaseolusvulgaris. Plant Pathology 45: 479–85. DOI: https://doi.org/10.1046/j.1365-3059.1996.d01-148.x

Taylor J D, Conway J, Roberts S J, Astley D and Vicente J G. 2002. Sources and origin of resistance to Xanthomonas campestris pv. campestris in Brassica genomes. Phytopathology 92: 105–11.

Teverson D M. 1991. ‘Genetics of pathogenicity and resistance in thehalo-blight disease of beans in Africa’. Ph D thesis, University of Birmingham, UK.

Vicente J G, Conway J, Roberts S J and Taylor J D. 2001. Identificationand origin of Xanthomonas campestrispv. campestris races andrelated pathovars. Phytopathology 91: 492–9. DOI: https://doi.org/10.1094/PHYTO.2001.91.5.492

Vicente J G, Taylor J D, Sharpe A G, Parkin I A P, Lydiate D J and King G J.2002. Inheritance of race-specific resistance to Xanthomonas campestris pv. campestris in Brassica genomes. Phytopathology 92:1 134–41. DOI: https://doi.org/10.1094/PHYTO.2002.92.1.105

Vicente J G, Everett B and Roberts S J. 2006. Identification of isolates that cause a leaf spot disease of brassicas as Xanthomonas campestris pv. raphani and pathogenic and genetic comparison with related pathovars. Phytopathology 96: 735–45. DOI: https://doi.org/10.1094/PHYTO-96-0735

Westman A L.1998. ‘Molecular marker variation and disease reaction to Alternariabrassicicola and Xanthomonas campestris pv. campestris in Brassica nigra and other crucifer species’. Ph D thesis, Cornell University, Ithaca, New York, 15 p.