Analysis of population structure and genetic relatedness among root (wilt) disease-resistant and susceptible west coast tall coconut palms (Cocos nucifera) using microsatellite markers
277 / 63
Authors
-
K Deva Kumar
Central Agricultural Research Institute, Port Blair
-
R J Thomas
CPCRI Regional Station, Kayangulam
-
R V Nair
CPCRI, Kasaragod
-
A B Jerard
CPCRI, Kasaragod
-
M K Rajesh
CPCRI, Kasaragod
-
P M Jacob
CPCRI Regional Station, Kayangulam
-
K Jayadev
Alvas College, Moodbidri, DK dist, Karnataka
-
V A Parthasarathy
IISR, Kozhikode
Keywords:
Coconut, Genetic relatedness, Microsatellites, Resistance breeding, Root (wilt) diseaseAbstract
The population structures among the root (wilt) disease-resistant and susceptible coconut palms from 12 locations in the three disease-endemic districts of southern Kerala, were analyzed using nine microsatellite markers. The pair-wise population differentiation estimate (Fst) between the resistant and susceptible populations was 0.021. Two major populations and a subpopulation cluster were identified among the resistant palms. The analysis of genetic relatedness between the resistant mother palms showed that most of the palms located in a single locality shared sib relationship among them. The existence of close genetic relationship among resistant palms from Chengannur, Thiruvalla, Kottayam and Pavukkara localities in Kerala were reported. The results are discussed in terms of breeding strategies for increasing heterozygosity and obtaining the maximum number of disease-resistant seedlings.
Downloads
References
Bassam B J and Caetano-Anollés G. 1993. Silver staining of DNA in polyacrylamide gels. Applied Biochemistry and
Biotechnology 42: 181–8.
CPCRI. 1985. A Survey Report - Coconut Root (Wilt) Disease - Intensity, Production Loss and Future Strategy, pp 45. Central Plantation Crops Research Institute, Kasaragod, Kerala.
CPCRI 1991. Annual Report 1990–91, 154. pp Central Plantation Crops Research Institute, Kasaragod, Kerala.
Evanno G, Regnaut S and Goudat J. 2005. Detcting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology 14: 2611–20.
Kalinowski, S T, Wagner A P and Taper M L. 2006. ML-Relate: acomputer program for maximum likelihood estimation ofrelatedness and relationship. Molecular Ecology Notes 6: 576–9.
Liu J. 2001. POWERSSR—A microsatellite data analysis softwarev 1.2.1. North Carolina State University, Raleigh, NC, USA.
Mathen K, Solomon J J, Rajan P and Geetha L. 1987. Electronmicroscopic evidence on the Stephanitis typica (Distant) as the vector of coconut root (wilt). Current Science 56:1239–40.
Nair M K, Koshy P K and Jacob P M. 1996. A root (wilt) resistant coconut hybrid and strategy for resistance breeding. Indian Coconut Journal 27: 2–5.
Nair R V, Jacob P M, Koshy P K, Sasikala M and Nampoothiri K UK. 2002. Breeding for resistance/tolerance to coconut root (wilt)disease. (in). Plantation Crops Research and Development inthe New Millennium, pp 67–71.
Rethinam P, Khan, H H, Reddy V M, Mandal P K and Suresh K (Eds). Proceedings PLACROSYM XIV. 12–15 December 2000, Hyderabad, Coconut Development Board, Kochi, Kerala.
Nei M. 1972 Genetic distance between populations. American Naturalist 106: 283–91.
Peakal R and Smouse P E. 2001. GENALEX V 5.0. Genetic analysis in Excel. Population genetic software for teaching and research. Australian National University, Canberra, Australia. Available at http://www.anu.edu.au/BoZo/GenAlEx/.
Perera L, Russell J R, Provan J and Powell W. 2000. Use of
microsatellite DNA markers to investigate the level of genetic diversity and population genetic structure of coconut (Cocos nucifera L.). Genome 43: 15–21.
Pritchard J K, Stephens M and Donnelly P. 2000. Inference of population structure using multilocus genotype data. Genetics 155: 945–59.
Solomon J J, Govindankutty M P and Nienhaus F. 1983. Association of mycoplasma like organisms with the coconut root (wilt) disease in India. Z. Pflkrankh. Pflschutz 90: 295–7.
Upadhyay A, Parthasarathy V A, Seema G and Karun A. 1999. An efficient method of DNA extraction from coconut. Agrotropica 11 (1): 35–8.
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2014 The Indian Journal of Agricultural Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Agricultural Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.
How to Cite
