Genetic diversity in natural watermelon (Citrullus lanatus) populations of Rajasthan

JITENDRA KUMAR BHAKAR; R SHARMA; PRAVEEN K SINGH; G SINGH

doi:10.56093/ijas.v87i6.71011

Authors

JITENDRA KUMAR BHAKAR Plant Biotechnology Center, Rajasthan Agricultural University, Beechwal, Bikaner, Rajasthan 334 006
R SHARMA Plant Biotechnology Center, Rajasthan Agricultural University, Beechwal, Bikaner, Rajasthan 334 006
PRAVEEN K SINGH Plant Biotechnology Center, Rajasthan Agricultural University, Beechwal, Bikaner, Rajasthan 334 006
G SINGH Plant Biotechnology Center, Rajasthan Agricultural University, Beechwal, Bikaner, Rajasthan 334 006

https://doi.org/10.56093/ijas.v87i6.71011

Keywords:

Crop improvement, Ecotypes, Genetic diversity, Natural population, RAPD, Watermelon

Abstract

The present investigation was carried out to estimate the genetic diversity at molecular level using RAPD primers across 23 genotypes of watermelon [ Citrullus lanatus (Thunb.) Mastum and Nakai]. RAPD analysis was conducted using 14 random primers of which eight primers produced 41 bands, 39 being polymorphic, generating 95% polymorphism. Jaccard similarity index based on RAPD revealed 49.6% average similarity with a range of 28% to 86%. The SAHN clustering based on UPGMA algorithms has put genotypes in two groups, one with 5 genotypes and another with 9 genotypes. The 9 genotype were left separately these being most diverse. High within and between group diversity resulted in poor grouping. Moreover clustering did not show any regional pattern. However, some population specific bands suggested regional influence on diversity.

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