A heterotrophic nitrification - Aerobic denitrification bacterium

QINGPING HU; FENG HE; CAIFANG WEN

doi:10.56093/ijas.v88i6.80546

Authors

QINGPING HU College of Life Science, Shanxi Normal University, 1 Gongyuan Street, Linfen City 041 004, China
FENG HE College of Life Science, Shanxi Normal University, 1 Gongyuan Street, Linfen City 041 004, China
CAIFANG WEN College of Life Science, Shanxi Normal University, 1 Gongyuan Street, Linfen City 041 004, China

https://doi.org/10.56093/ijas.v88i6.80546

Keywords:

Aerobic denitriï¬cation, Heterotrophic nitriï¬cation, Identification, Nitrogen removal

Abstract

A bacterial strain named Pseudomonas stutzeri ZH-1, which exhibits the ability of efficient heterotrophic nitrification and aerobic denitrification, was isolated from 20 bacterial strains in the sludge of Fenhe River (in Shanxi Province, China) and identified by 16S-rDNA sequencing as a strain of Pseudomonas stutzeri. The cells of strain ZH-1 were Gram-negative, non-motile and short rod-shaped. In order to further understand its removal ability, several experiments were conducted to identify the growth and nitrogen removal response at different factors. The results showed that the optimum conditions is sodium citrate as carbon source, C/N 12, shaking speed >200 rpm, pH 9, temperature 25-35Â°C and nitrogen concentration >150mg/L. The average removal rate for NH₄+ _-N, NO₃ - _-N, NO₂ - _-N can be as high as 4.27, 5.53 and 4.30 mg/L/h for synthetic wastewater, respectively. In particular, the strain ZH-1 showed an amazing ability of rapid degradation for NH₄ + _-N , NO₃ - _-N and NO₂ - _-N under aerobic conditions and it might be a suitable candidate to simultaneously aerobic nitrification/denitrification for future full-scale applications in wastewater treatment.

Downloads

Download data is not yet available.

References

Chang C Y, Tanong K, Xu J and Shon H. 2011. Microbial community analysis of an aerobic nitrifying-denitrifying MBR treating ABS resin wastewater. Bioresource Technology 102(9): 5337–44. DOI: https://doi.org/10.1016/j.biortech.2010.12.045

Chen Q and Ni J. 2012. Ammonium removal by Agrobacterium sp. LAD9 capable of heterotrophic nitrification-aerobic denitrification. Journal of Bioscience and Bioengineering 113(5): 619–23. DOI: https://doi.org/10.1016/j.jbiosc.2011.12.012

Daniel L M C, Pozzi E, Foresti E and Chinalia F A. 2009. Removal of ammonium via simultaneous nitrification-denitrification nitrite-shortcut in a single packed-bed batch reactor. Bioresource Technology 100(3): 1100–7. DOI: https://doi.org/10.1016/j.biortech.2008.08.003

Fu Z, Yang F, An Y and Xue Y. 2009. Simultaneous nitrification and denitrification coupled with phosphorus removal in an modified anoxic/oxic-membrane bioreactor (A/O-MBR). Biochemical Engineering Journal 43(2): 191–6. DOI: https://doi.org/10.1016/j.bej.2008.09.021

Gupta A B and Gupta S K. 2001. Simultaneous carbon and nitrogen removal from high strength domestic wastewater in an aerobic RBC biofilm. Water Research 35(7): 1714–22. DOI: https://doi.org/10.1016/S0043-1354(00)00442-5

Garrity G M, Bell J A and Lilburn T G. May 2004. Bergey's Manual of Systematic Bacteriology. Taxonomic Outline of the Prokaryotes, Second edition, Release 5.0. Springer-Verlag, New York.

Huang T, Guo L, Zhang H, Su J, Wen G and Zhang K. 2015. Nitrogen-removal efficiency of a novel aerobic denitrifying bacterium, Pseudomonas stutzeri strain ZF31, isolated from a drinking-water reservoir. Bioresource Technology 196: 209–16. DOI: https://doi.org/10.1016/j.biortech.2015.07.059

Huang X, Li W, Zhang D and Qin W. 2013. Ammonium removal by a novel oligotrophic Acinetobacter sp. Y16 capable of heterotrophic nitrification-aerobic denitrification at low temperature. Bioresource Technology 146: 44–50. DOI: https://doi.org/10.1016/j.biortech.2013.07.046

He T, Li Z, Sun Q, Xu Y and Ye Q. 2016. Heterotrophic nitrification and aerobic denitrification by Pseudomonas tolaasii Y-11 without nitrite accumulation during nitrogen conversion. Bioresource Technology 200: 493–9. DOI: https://doi.org/10.1016/j.biortech.2015.10.064

Ji Z and Chen Y. 2010. Using sludge fermentation liquid to improve wastewater shortcut nitrification-denitrification and denitrifying phosphorus removal via nitrite. Environmental Science & Technology 44(23): 8957–63. DOI: https://doi.org/10.1021/es102547n

Joo H S, Hirai M and Shoda M. 2006. Piggery wastewater treatment using Alcaligenes faecalis strain No. 4 with heterotrophic nitrification and aerobic denitrification. Water Research 40: 3029–36. DOI: https://doi.org/10.1016/j.watres.2006.06.021

Joo H S, Hirai M and Shoda M. 2005. Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis No. 4. Journal of Bioscience and Bioengineering 100(2): 184–91. DOI: https://doi.org/10.1263/jbb.100.184

Janek T, Łukaszewicz M and Krasowska A. 2013. Identification and characterization of biosurfactants produced by the Arctic bacterium Pseudomonas putida BD2. Colloids and Surfaces B: Biointerfaces 110: 379–386. DOI: https://doi.org/10.1016/j.colsurfb.2013.05.008

Kim J K, Park K J, Cho K S, Nam S W, Park T J and Bajpai R. 2005. Aerobic nitrification-denitrification by heterotrophic Bacillus strains. Bioresource Technology 96(17): 1897–1906. DOI: https://doi.org/10.1016/j.biortech.2005.01.040

Khardenavis A A, Kapley A and Purohit H J. 2007. Simultaneous nitrification and denitrification by diverse Diaphorobacter sp. Applied Microbiology and Biotechnology 77(2): 403–9. DOI: https://doi.org/10.1007/s00253-007-1176-5

Khin T and Annachhatre A P. 2004. Novel microbial nitrogen removal processes. Biotechnology Advances 22: 519–32. DOI: https://doi.org/10.1016/j.biotechadv.2004.04.003

Lin L, Yuan S, Chen J, Xu Z and Lu X. 2009. Removal of ammonia nitrogen in wastewater by microwave radiation. Journal of Hazardous Materials 161(2): 1063–8. DOI: https://doi.org/10.1016/j.jhazmat.2008.04.053

Obaja D, Mace S and Mata-Alvarez J. 2005. Biological nutrient removal by a sequencing batch reactor (SBR) using an internal organic carbon source in digested piggery wastewater. Bioresource Technology 96(1): 7–14. DOI: https://doi.org/10.1016/j.biortech.2004.03.002

Patureau D, Godon J J, Dabert P, Bouchez T, Bernet N, Delgenes J P and Moletta R. 1998. Microvirgula aerodenitrificans gen. nov., sp. nov., a new gram-negative bacterium exhibiting co-respiration of oxygen and nitrogen oxides up to oxygen-saturated conditions. International Journal of Systematic and Evolutionary Microbiology 48(3): 775–82. DOI: https://doi.org/10.1099/00207713-48-3-775

Padhi S K, Tripathy S, Sen R, Mahapatra A S, Mohanty S and Maiti N K. 2013. Characterisation of heterotrophic nitrifying and aerobic denitrifying Klebsiella pneumoniae CF-S9 strain for bioremediation of wastewater. International Biodeterioration and Biodegradation 78: 67–73. DOI: https://doi.org/10.1016/j.ibiod.2013.01.001

Robertson L A, Van Niel E W, Torremans R A and Kuenen J G. 1988. Simultaneous nitrification and denitrification in aerobic chemostat cultures of Thiosphaera pantotropha. Applied and Environmental Microbiology 54(11): 2812–8. DOI: https://doi.org/10.1128/aem.54.11.2812-2818.1988

Saricheewin K, Sirivithayapakorn S, Noophan P L, Wantawin C, Techkarnjanaruk S and Munakata-Marr J. 2010. Nitrogen removal of anammox cultures under different enrichment conditions. Journal of Environmental Science and Health Part A 45:1832–8. DOI: https://doi.org/10.1080/10934529.2010.520498

Schmidt I, Slieker O, Schmid M, Bock E, Fuerst J, Kuenen J G, and Strous M. 2003. New concepts of microbial treatment processes for the nitrogen removal in wastewater. FEMS Microbiology Reviews 27(4): 481–92. DOI: https://doi.org/10.1016/S0168-6445(03)00039-1

Song Z F, An J, Fu G H and Yang X L. 2011. Isolation and characterization of an aerobic denitrifying Bacillus sp. YX-6 from shrimp culture ponds. Aquaculture 319(1): 188–93. DOI: https://doi.org/10.1016/j.aquaculture.2011.06.018

Takebe F, Hirota K, Nodasaka Y and Yumoto I. 2012. Brevibacillus nitrificans sp. nov., a nitrifying bacterium isolated from a microbiological agent for enhancing microbial digestion in sewage treatment tanks. International Journal of Systematic and Evolutionary Microbiology 62: 2121–6. DOI: https://doi.org/10.1099/ijs.0.032342-0

Taylor S M, Yiliang H E, Bin Z and Huang J. 2009. Heterotrophic ammonium removal characteristics of an aerobic heterotrophic nitrifying-denitrifying bacterium, Providencia rettgeri YL. Journal of Environmental Sciences 21(10): 1336–1. DOI: https://doi.org/10.1016/S1001-0742(08)62423-7

Wan C, Yang X, Lee D J, Du M, Wan F and Chen C. 2011. Aerobic denitrification by novel isolated strain using as nitrogen source. Bioresource Technology 102(15): 7244–8. DOI: https://doi.org/10.1016/j.biortech.2011.04.101

Yao S, Ni J, Chen Q and Borthwick A G. 2013. Enrichment and characterization of a bacteria consortium capable of heterotrophic nitrification and aerobic denitrification at low temperature. Bioresource Technology 127: 151–7. DOI: https://doi.org/10.1016/j.biortech.2012.09.098

Zhang J, Wu P, Hao B and Yu Z. 2011. Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001. Bioresource Technology 102(21): 9866–9. DOI: https://doi.org/10.1016/j.biortech.2011.07.118

Zhang Q L, Liu Y, Ai G M, Miao L L, Zheng H Y and Liu Z P. 2012. The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium, Bacillus methylotrophicus strain L7. Bioresource Technology 108: 35–44. DOI: https://doi.org/10.1016/j.biortech.2011.12.139

Zhu L, Ding W, Feng L J, Kong Y, Xu J and Xu X Y. 2012. Isolation of aerobic denitrifiers and characterization for their potential application in the bioremediation of oligotrophic ecosystem. Bioresource Technology 108: 1–7. DOI: https://doi.org/10.1016/j.biortech.2011.12.033