Bioefficacy of various strains of Trichoderma and Pseudomonas spp. against damping-off of cauliflower

AJAY KUMAR CHOUDHARY; NARINDER SINGH; DALJEET SINGH; SHIVETA RAINA

doi:10.56093/ijas.v89i2.87012

Authors

AJAY KUMAR CHOUDHARY Ph D Student, Punjab Agricultural University, Ludhiana, Punjab 141 004
NARINDER SINGH Senior Plant Pathologist, Punjab Agricultural University, Ludhiana, Punjab 141 004
DALJEET SINGH Senior Plant Pathologist, Punjab Agricultural University, Ludhiana, Punjab 141 004
SHIVETA RAINA Research Fellow, Punjab Agricultural University, Ludhiana, Punjab 141 004

https://doi.org/10.56093/ijas.v89i2.87012

Keywords:

Cauliflower, Damping-off, P. aphanidermatum, P. fluorescens, Trichoderma spp.

Abstract

Trichoderma and Pseudomonas strains were tested against damping-off of cauliflower (Pythium aphanidermatum and Rhizoctonia solani). Dual culture antagonism assays were carried out on potato dextrose agar and Kingâ€™s B medium to determine the effect of Trichoderma and Pseudomonas strains respectively, on mycelial growth of the pathogens. Antagonistic effect on P. aphanidermatum and R. solani was 53.1 and 44.6% respectively by T. harzianum (Th5) where as P. fluorescens (Pf3) showed maximum inhibition against P. aphanidermatum and R. solani of 60 and 70% respectively. Finally, the selected potent strains of Trichoderma spp (Th5 and Tv1) and P. fluorescens Pf3, were evaluated on cauliflower seedling development and as a potential antagonist for controlling cauliflower dampingoff caused by P. aphanidermatum and R.solani, under field conditions. T. harzianum (Th5) resulted significantly reduction in damping off disease, promoted the plant growth and enhanced the yield.

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References

Abdel K M M. 1997. Field application of Trichoderma harzianum as biocide for control bean root rot disease. Egyptian Journal of Phytopathology 25: 19–25.

Abdelzaher H M A. 2004. Occurrence of damping-off of wheat caused by Pythium diclinum Tokunaga in El-Minia, Egypt and its possible control by Glocladium roseum and Trichoderma harzianum. Archives of Phytopathology and Plant Protection 37: 147–59. DOI: https://doi.org/10.1080/0323540042000205893

Ahmed J S and Baker R. 1987. Competitive saprophytic ability and cellulolytic activity of rhizosphere competent mutants of Trichoderma harzianum. Phytopathology 77: 358–62. DOI: https://doi.org/10.1094/Phyto-77-358

Andersen J B, Koch B, Nielsen T H, Sorensen D, Hansen M, Nybroe O, Christophersen C, Sorensen J, Molin S and Givskov M. 2003. Surface motility in Pseudomonas sp. DSS73 is required for efficient biological containment of the root-pathogenic microfungi Rhizoctonia solani and Pythium ultimum. Microbiology 149: 37–46. DOI: https://doi.org/10.1099/mic.0.25859-0

Carisse O, Bernier J and Benhamou N. 2003. Selection of biological agents from composts for control of damping-off of cucumber caused by Pythium ultimum. Candian Journal of Plant Pathology 25: 258–67. DOI: https://doi.org/10.1080/07060660309507078

Dey T K. 2005. Effect of soil solarization in controlling damping-off disease of true potato seedlings. Bangladesh Journal of Plant Pathology 21: 93.

Dixit R, Singh R B and Singh H B. 2015. Screening of antagonistic potential and plant growth promotion activities of Trichoderma spp. and fluorescent Pseudomonas spp. isolates against Sclerotinia sclerotiorum causing stem rot of French bean. Legume Research. 38: 375–81. DOI: https://doi.org/10.5958/0976-0571.2015.00121.6

Elad Y, Chet I and Henis Y. 1982. Degradation of plant pathogenic fungi by Trichoderma harzianum. Canadian Journal of Microbiology 28: 719–25. DOI: https://doi.org/10.1139/m82-110

Faruk M I. 2016. Substrate based Trichoderma harzianum bio-fungicides control damping-off disease (Rhizoctonia solani) of cauliflower in seed bed. International Journal of Innovative and Applied Research 4: 1–10. DOI: https://doi.org/10.18483/ijSci.617

Gravel V, Martinez C, Antoun H and Tweddell R J. 2004. Antagonistic microorganisms with the ablity to control Pythium damping-off of tomato seeds in rock wool. Bio Control 50: 771–86. DOI: https://doi.org/10.1007/s10526-005-1312-z

Harman G E. 1991. Seed treatments for biological control of plant disease. Crop Protection 10: 166–71. DOI: https://doi.org/10.1016/0261-2194(91)90038-S

Harman G E, Howell C R, Viterbo A, Chet I and Lorito M. 2004. Trichoderma species-opportunistic, a virulent plant symbionts. Nature Review Microbiology 2: 43–56. DOI: https://doi.org/10.1038/nrmicro797

Holt J G, Krieg R N, Sneath A H P, Staley T J and Williams T S. 1994. Bergey's Manual of Determinative Bacteriology, 9th edn. Baltimore, Maryland, USA.

Jayaraj J, Radhakrishnan N V and Velazhahanpages R. 2006. Development of formulations of Trichoderma harzianum strain M1 for control of damping-off of tomato caused by Pythium aphanidermatum. Archives of Phytopathology and Plant Protection 39: 1–8. DOI: https://doi.org/10.1080/03235400500094720

Kamlesh M and Gujar R S. 2002. Evaluation of different fungal antagonistic, plant extracts and oil cakes against Rhizoctonia solani causing stem rot of chilli seedlings. Annals of Plant Protection Sciences 10: 319–22.

King E O, Ward M K and Raney D E. 1954. Two simple media for the demonstration of pyocyanine and fluorescein. Journal of Laboratory and Clinical Medicine 44: 301–7.

Kirsh V A, Peters U, Mayne S T, Subar A F, Chatterjee N, Johnson C C and Hayes R B. 2007. Prospective study of fruit and vegetable intake and risk of prostate cancer. Journal of the National Cancer Institute 99: 1200–9. DOI: https://doi.org/10.1093/jnci/djm065

Kucuk C and Kivanc M. 2003. Isolation of Trichoderma spp. and determination their antifungal and biochemical, physiological features. Turkish Journal of Biology 27: 247–53.

Leclère V, Béchet M, Adam A, Jean-Sébastien G, Wathelet B, Ongena M, Thonart P, Gancel F, Chollet-Imbert M and Jacques P. 2005. Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism's antagonistic and biocontrol activities. Applied and Environmental Microbiology 71: 4577–84. DOI: https://doi.org/10.1128/AEM.71.8.4577-4584.2005

Manoranjithum S K, Prakasam V, Rajappan K and Amutha G. 2000. Control of chilli damping off using bioagents. Journal of Mycology and Plant Pathology 30: 225–8.

Maurya M K, Singh R and Tomer A. 2014. In vitro evaluation of antagonistic activity of Pseudomonas fluorescens against fungal pathogen. Journal of Biopesticides 7: 43–6.

Moller K, Jensen B, Andersen H P, Stryyhn H and Hockenhull J. 2003. Biocontrol of Pythium tracheiphilum in Chinese cabbage by Clonostachs rosea under field conditions. Biocontrol Science and Technology 13: 171–82. DOI: https://doi.org/10.1080/958315021000073448

Muhammad S and Amusa N A. 2003. In vitro inhibition of growth of some seedling blight inducing pathogens by compost-inhabiting microbes. African Journal of Biotechnology 2: 161–4. DOI: https://doi.org/10.5897/AJB2003.000-1033

Mukhtar I. 2008. Influence of Trichoderma species on seed germination in okra. Mycopathology 1: 45–50.

Okoth S A, Otadoh J A and Ocha J O. 2011. Improved seedling emergence, growth of maize, beans by Trichoderma harziunum. Tropical and Subtropical Agroecosystems 13: 65–71.

Rini C R and Sulochana K K. 2006. Management of seedling rot of chilli (Capsicum annuum L.) using Trichoderma spp., Pseudomonas fluorescens. Journal of Tropical Agriculture 44: 79–82.

Rosenzweig C, EIglesias A, Yang X B, Epstein P R and Chivian E. 2001. Climate change, extreme weather events: implications for food production, plant diseases, pests. Global Change and Human Health 2: 90–104. DOI: https://doi.org/10.1023/A:1015086831467

Seema M and Devaki N S. 2010. Effect of some essential oils on Rhizoctonia solani Kuhn infecting flue-cured Virginia tobacco. Journal of Biopesticides 3: 563–6.

Shabir R and Rubina L. 2010. Biological control of damping-off disease of cabbage caused by Rhizoctonia solani Kuhn. Applied Biological Research 12: 38–41.

Sharma P and Sain S K. 2005. Use of biotic agents, abiotic compounds against damping off of cauliflower caused by Pythium aphanidermatum. Indian Phytopathology 58: 395–401.

Singh A, Shahid M, Kumar V and Srivastava M. 2012. Evaluation of different substrate for mass multiplication of Trichoderma spp. Trends in Biosciences 5: 68–70.

Sivan A and Chet I. 1986. Biological control of Fusarium spp. in cotton, wheat, muskmelon by Trichoderma harzianum. Journal of Phytopathology 116: 39-47. DOI: https://doi.org/10.1111/j.1439-0434.1986.tb00892.x

Stephan Z A, Jbara I M and Al-Rawi F A. 2003. Effect of soil moisture content, thermal treatment on the activity of bioagent fungi Trichoderma harzianum Rifai, Paecilomayces lilacinus (thom) Samson on some tomato plant growth parameters. Arabian Journal of Plant Protection 21: 1–5.

Tjamos E C, Papavizas G C and Cook R J. 1992. Biological control of plant diseases: Progress and challenges for the future. New York, USA DOI: https://doi.org/10.1007/978-1-4757-9468-7

We W S, Liu S D and Tschen S. 1986. Hyperparasitic relationship between antagonists, Rhizoctonia solani. Plant Protection Bulletin 28: 91–100.