Physio-biochemical responses of hybrid citrus rootstock progenies to NaCl induced salinity


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Authors

  • PRASHANT KALAL Ph D Scholar, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • R M SHARMA Principal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • A K DUBEY Principal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • DEEBA KAMIL Scientist, Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • S LEKSHMY Scientist, Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • AMRENDER KUMAR Senior Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • O P AWASTHI Principal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India

https://doi.org/10.56093/ijas.v89i6.90817

Keywords:

Citrus hybrid, Lipid, Peroxidation, Proline, Salinity, Superoxide

Abstract

The study consisted 30 hybrids of Pummelo (P) × Troyer (T) with two check cultivars, viz. Attani-2 and Troyer citrange, were subjected to 100 mM NaCl treatment through irrigations till the appearance of foliar symptoms. Of the 30 hybrids, only 3 hybrids, viz. P × T-86, P × T-98 and P × T -102 showed higher photosynthetic rate (A) (6.22 -6.42 μmol m2/s) than other hybrids. Furthermore, the level of O2 - was lowest in Attani-2. The lowest Cl- content was noticed in P × T-102 (0.03%) followed by P × T-98 (0.04%). Of the 30 hybrids (Pummelo × Troyer citrange), evaluated against 100 mM NaCl induced salinity, 3 hybrids P × T-86, P × T-98 and P × T-102 had very low scorching of leaves (32.88-33.15%), and were found tolerant as these hybrids expressed the low level of lipid peroxidation and Cl- accumulation in leaves, besides maintaining the higher A and MSI than other hybrids and Troyer citrange.

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References

Adriano D C and Doner H E. 1982. Bromine, chlorine, and fluorine. Methods of Soil Analysis. Part II. Chemical and microbiological properties, pp 461–2. Page A L, Miller R H and Keency D R (Eds.). Soil Science Society of America and American Society of Agronomy. Madison,Wisconsin, USA.

Anjum M A. 2008. Effect of NaCl concentrations in irrigation water on growth and polyamine metabolism in two citrus rootstocks with different levels of salinity tolerance. Acta PhysiologiaePlantarum 30: 43–52. DOI: https://doi.org/10.1007/s11738-007-0089-3

Anonymous. 2016. Horticultural Statistics at a Glance 2015. Ministry of Agriculture & Farmers Welfare, Government of India 2016. Ministry of Agriculture & Farmers Welfare, Government of India.

Arbona V, Flors V, Jacas J, Garcia-Agustin P and Gomez-Cadenas A. 2003. Enzymatic and non-enzymatic antioxidant responses of Carrizo citrange, a salt-sensitive citrus rootstock, to different levels of salinity. Plant Cell Physiology 44: 388–94. DOI: https://doi.org/10.1093/pcp/pcg059

Arbona V, Lopez-Climent M F, Mahouachi J, Perez-Clemente RM, Abrams SR and Gomez-Cadenas A. 2006. Use of persistent analogs of abscisic acid as palliatives against salt-stress induced damage in citrus plants. Journal of Plant Growth Regulation 25: 1–9. DOI: https://doi.org/10.1007/s00344-005-0038-6

Banuls J and Primo‐Millo E. 1992. Effects of chloride and sodium on gas exchange parameters and water relations of citrus plants. Physiologia Plantarum 86: 115–23. DOI: https://doi.org/10.1034/j.1399-3054.1992.860116.x

Banuls J, Serna M D, Legaz F, Talon M and Primo-Millo E . 1997. Growth and gas exchange parameters of Citrus plants stressed with different salts. Journal of Plant Physiology 150: 194–9. DOI: https://doi.org/10.1016/S0176-1617(97)80202-7

Bates L S, Waldren R P and Teare I D. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil 39: 205–7. DOI: https://doi.org/10.1007/BF00018060

Aouad B A E, Fadli A, Tarik A, Abdelhak T, Benkirance R and Benyahia H. 2015. Investigating salt tolerance in citrus rootstocks under greenhouse conditions using growth and biochemical indicators. Biolife 3: 827–37.

Cai Q, Guy C L and Moore G A. 1994. Extension of the linkage map in citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold-acclimation-responsive loci. Theoretical and Applied Genetics 89: 606–14. DOI: https://doi.org/10.1007/BF00222455

Castle W S. 2010. A career perspective on citrus rootstocks, their development, and commercialization. Horticultural Science 45: 11–5. DOI: https://doi.org/10.21273/HORTSCI.45.1.11

Chaitanya K K andNaithani S C. 1994. Role of superoxide, lipid peroxidation and superoxide dismutase in membrane perturbation during loss of viability in seeds of Shorearobusta Gaertn. f. New Phytology 126: 623–7. DOI: https://doi.org/10.1111/j.1469-8137.1994.tb02957.x

Chen S Y. 1991. Sources of proline nitrogen in water- stressed soybean (Glycine max). II. Fate of 15N-labelled protein. Physiologia Plantarum 61: 622–8.

Conn S and Gilliham M. 2010. Comparative physiology of elemental distributions in plants. Annals of Botany 105: 1081–102. DOI: https://doi.org/10.1093/aob/mcq027

Fukutaku Y and Yamada Y. 1984. Sources of proline nitrogen in water-stressed soybean (Glycine max). II. Fate of 15N-labelled protein. Physiologia Plantarum 61: 622–8. DOI: https://doi.org/10.1111/j.1399-3054.1984.tb05180.x

Garcia-Sanchez F, Jifon J L, Carvajal M and Syvertsen J P. 2002. Gas exchange, chlorophyll and nutrient contents in relation to Na+ and Cl- accumulation in Sunburst mandarin grafted on different rootstocks. Plant Science 162: 705–12. DOI: https://doi.org/10.1016/S0168-9452(02)00010-9

Garcia-Sanchez F and Syvertsen J P. 2006. Salinity tolerance of Cleopatra mandarin and Carrizo citrange citrus rootstock seedlings is affected by CO2 enrichment during growth. Journal of the American Society for Horticultural Science 131: 24–31. DOI: https://doi.org/10.21273/JASHS.131.1.24

Gonzalez P, Syvertsen J P and Etxeberria E. 2012. Sodium distribution in salt-stressed citrus rootstock seedlings. Horticultural Science 47: 1504–11. DOI: https://doi.org/10.21273/HORTSCI.47.10.1504

Heath R L and Packer L. 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125: 189–98. DOI: https://doi.org/10.1016/0003-9861(68)90654-1

Herrero R, Asins M J, Carbonell E A and Navarro L. 1996. Genetic diversity in the orange subfamily Aurantioideae. I. Intraspecies and intragenus genetic variability. Theoretical and Applied Genetics 92: 599–609. DOI: https://doi.org/10.1007/BF00224564

Jackson M L. 1980. Soil Chemical Analysis. Prentice Hall of India Pvt Ltd, New Delhi. p. 452.

Kardpol R P and Rao N A. 1985. Alterations in the biosynthesis of proteins and nucleic acids in linger millet (Eleusine coracana) seedlings during water stress and effect of proline on protein biosynthesis. Plant Science 40: 73–9. DOI: https://doi.org/10.1016/0168-9452(85)90044-5

Katsuhara M, Otsuka T and Ezaki B. 2005. Salt stress-induced lipid peroxidation is reduced by glutathione S-transferase, but this reduction of lipid peroxides is not enough for a recovery of root growth in Arabidopsis. Plant Science 169: 369–73. DOI: https://doi.org/10.1016/j.plantsci.2005.03.030

Lopez-Climent M F, Arbona V, Perez-Clemente R M and Gomez- Cadenas A. 2008. Relationship between salt tolerance and photosynthetic machinery performance in citrus. Environmental and Experimental Botany 62: 176–84. DOI: https://doi.org/10.1016/j.envexpbot.2007.08.002

Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7: 405–10. DOI: https://doi.org/10.1016/S1360-1385(02)02312-9

Nieves M, Cerda A and Botella M. 1991. Salt tolerance of two lemon scions measured by leaf chloride and sodium accumulation. Journal of Plant Nutrition 14: 623–36. DOI: https://doi.org/10.1080/01904169109364229

Paleg L G, Stewart G R and Bredbeer J W. 1984. Proline and glycine betaine influence protein salvation. Plant Physiology 75: 974–8. DOI: https://doi.org/10.1104/pp.75.4.974

Paranychianakis NV andChartzoulakis KS. 2005. Irrigation of Mediterranean crops with saline water: from physiology to management practices. Agriculture, Ecosystems & Environment 106: 171–87. DOI: https://doi.org/10.1016/j.agee.2004.10.006

Patel S K, Dubey A K, Srivastav M, Singh A K, Dahuja A and Pandey RN. 2011. Effect of NaCl in the irrigation water on growth, anti-oxidant enzyme activities, and nutrient uptake in five citrus rootstocks. Journal of Horticultural Science and Biotechnology 86: 189–95. DOI: https://doi.org/10.1080/14620316.2011.11512746

Pitzschke A, Forzani C and Hirt H. 2006. Reactive oxygen species signaling in plants. Antioxidanta & Redox Signalling 8: 1757–64. DOI: https://doi.org/10.1089/ars.2006.8.1757

Raga V, Bernet G P, Carbonell E A andAsins M J. 2014. Inheritance of rootstock effects and their association with salt tolerance candidate genes in a progeny derived from Volkamer lemon. Journal of the American Society for Horticultural Science 139: 1–11. DOI: https://doi.org/10.21273/JASHS.139.5.518

Sairam R K, Deshmukh P S and Shukla D S. 1997. Tolerance to drought and temperature stress in relation to increased antioxidant enzyme activity in wheat. Journal of Agronomy and Crop Science 178: 171–7. DOI: https://doi.org/10.1111/j.1439-037X.1997.tb00486.x

Singh A, Saini M L and Behl R K. 2003. Screening of citrus rootstocks for salt tolerance in semi-arid climates-A review. Tropics 13: 53–66. DOI: https://doi.org/10.3759/tropics.13.53

Smirnoff N and Cumbes QJ. 1989. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28: 1057–60. DOI: https://doi.org/10.1016/0031-9422(89)80182-7

Storey R and Walker RR. 1999. Citrus and salinity. Scientia Horticulturae 78: 39–81. DOI: https://doi.org/10.1016/S0304-4238(98)00190-3

Syvertsen J P and Smith Jr ML. 1983. Environmental stress and seasonal changes in proline concentration of citrus tree tissues and juice. Journal of the American Society for Horticultural Science 108: 861–6. DOI: https://doi.org/10.21273/JASHS.108.5.861

Tozlu I, Moore GA and Guy C L. 2000. Effects of increasing NaCl concentration on stem elongation, dry mass production, and macro-and micro-nutrient accumulation in Poncirus trifoliata. Functional Plant Biology 27: 35–42. DOI: https://doi.org/10.1071/PP99074

Tozlu I, Moore GA and Guy C L. 2000. Regulation of growth and differential tissue dry mass accumulation by Citrus grandis, Poncirus trifoliata and their F1 under salinized and non-salinized environments. Functional Plant Biology 27: 27–33. DOI: https://doi.org/10.1071/PP99060

Tozlu I, Guy CL and Moore GA. 2002. Tolerance mechanisms to salinity stress in Citrus and Poncirus. Acta Horticulturae 573: 271–82. DOI: https://doi.org/10.17660/ActaHortic.2002.573.31

Yesiloglu T, Incesu M, Yılmaz B, Tuzcu O, Kamiloglu M and Cimen B. 2015. Effects of salinity on some citrus rootstocks. Acta Horticulturae 1065: 1311–6. DOI: https://doi.org/10.17660/ActaHortic.2015.1065.166

Zhu J, Bie Z, Huang Y and Han X. 2008. Effect of grafting on the growth and ion concentrations of cucumber seedlings under NaCl stress. Soil Science and Plant Nutrition 54: 895–902. DOI: https://doi.org/10.1111/j.1747-0765.2008.00306.x

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2019-06-19

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2019-06-19

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KALAL, P., SHARMA, R. M., DUBEY, A. K., KAMIL, D., LEKSHMY, S., KUMAR, A., & AWASTHI, O. P. (2019). Physio-biochemical responses of hybrid citrus rootstock progenies to NaCl induced salinity. The Indian Journal of Agricultural Sciences, 89(6), 975–982. https://doi.org/10.56093/ijas.v89i6.90817
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