Identification of carbohydrate active enzymes from whole genome sequence of Tilletia indica and sporulation analysis

JAGMOHAN SINGH; RASHMI AGGARWAL; MALKHAN SINGH GURJAR; SAPNA SHARMA; M S SAHARAN

doi:10.56093/ijas.v89i6.90828

Authors

JAGMOHAN SINGH Ph D Scholar, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
RASHMI AGGARWAL Head, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
MALKHAN SINGH GURJAR Scientist SS, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
SAPNA SHARMA SRF, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
M S SAHARAN Principal scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India

https://doi.org/10.56093/ijas.v89i6.90828

Keywords:

Carbohydrate active enzymes, Karnal bunt, Sporulation, Tilletia indica, Wheat

Abstract

Karnal bunt of wheat is an internationally quarantined disease. One of the major constraints in exporting wheat is prevalence of Karnal bunt (KB). It is caused by Tilletia indica which is a heterothallic fungus and requires fusion of different mating types for causing infection. The growth efficiency and aggressiveness of plant pathogens are often associated with their carbohydrate active enzymes (CAZymes). The present study was undertaken to identify the CAZymes in whole genome sequence of Tilletia indica, and conduct sporulation analysis. Total 315 predicted secretory proteins were annotated with the CAZyme database (dbCAN). The secreted carbohydrates active enzymes were identified as 105 glycosylhydrolases (GH), 85 glycosyltransferases (GT), 83 carbohydrate esterases (CE), 8 carbohydrate binding modules (CBM), 30 auxiliary activities (AA) and 4 polysaccharide lyases (PL). Based on sporulation analysis of T. indica isolates, KB1 and KB2 isolates were most sporulative among all the isolates of T. indica. These carbohydrate active enzymes can help to understand the pathogenesis mechanism(s) of Karnal bunt using functional genomics approach.

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References

Aggarwal R, Singh D V and Srivastava K D. 1999. Studies on ontogeny of teliospore ornamentation of Neovossia indica observed through scanning electron microscopy. Indian Phytopathology 52(4): 417–9.

Aggarwal R, Tripathi A and Yadav A. 2010. Pathogenic and genetic variability in Tilletia indica monosporidial culture lines using universal rice primer-PCR. European Journal of Plant Pathology 128(3): 333–42. DOI: https://doi.org/10.1007/s10658-010-9655-4

Bonde M R, Berner D K, Nester S E, Peterson G L, Olsen M W, Cunfer B M and Sim T. 2004. Survival of Tilletia indica teliospores in different soils. Plant Disease 88:316–24. DOI: https://doi.org/10.1094/PDIS.2004.88.4.316

Dhaliwal H S and Singh D V. 1988.Up-to-date life cycle of Neovossia indica (Mitra) Mundkur. Current Science 57(12): 675–7.

FAO. 1996. International Standards for Phytosanitary Measures: Guidelines for Pest Risk Analysis. Food and Agriculture Organization of the United Nations, Rome, Italy.

Gurjar M S and Aggarwal R. 2018.Whole genome sequencing and secretome analysis of Tilletia indica fungus provides pathogenesis related genes. Phyto Path 105: S1–S101.

Gurjar M S, Aggarwal R, Sharma S, Kulshreshtha D, Gupta A, Gogoi R, Thirumalaisamy P P and Saini A. 2017. Development of real time PCR assay for the detection and quantification of teliospores of Tilletia indica causing wheat Karnal bunt in soil.Indian Journal of Experimental Biology 55(6): 549–54.

Gurjar M S, Jogawat A, Kulshreshtha D, Sharma S, Gogoi R and Aggarwal R. 2016. Intraspecific variation in Tilletia indica, a pathogen causing Karnal bunt of wheat. Indian Phytopathology 69(4): 352–6.

Krishna A and Singh R A. 1983. Cytology of teliospore germination and development in Neovossia indica the incitant of Karnal bunt of wheat. Indian Phytopathology 36(1): 115–23.

Lairson L L, Henrissat B, Davies G J and Withers S G. 2008. Glycosyltransferases: structures, functions, and mechanisms. Annual Reviewof Biochemistry 77: 521–55. DOI: https://doi.org/10.1146/annurev.biochem.76.061005.092322

MitraM. 1931. A new bunt on wheat in India. Annals of Applied Biology 18(2): 178–9. DOI: https://doi.org/10.1111/j.1744-7348.1931.tb02294.x

Nagarajan S, Aujla S S, Nanda G S, Sharma I, Goel L B, Kumar J and Singh D V.1997. Karnal bunt (Tilletia indica) of wheat–a review. Review of Plant Pathology 76(12): 1207–14.

Ospina-Giraldo M D, Mullins E and Kang S. 2003. Loss of function of the Fusariumoxysporum SNF1 gene reduces virulence on cabbage and Arabidopsis. Current Genomics 44(1): 49–57. DOI: https://doi.org/10.1007/s00294-003-0419-y

Ospina-Giraldo M D, McWalters J and Seyer L. 2010. Structural and functional profile of the carbohydrate esterase gene complement in Phytophthora infestans.Current Genomics 56(6): 495–506. DOI: https://doi.org/10.1007/s00294-010-0317-z

Singh D V and Gogoi R. 2011.Karnal bunt of wheat (Triticum spp.) a global scenario. Indian Journal of Agricultural Sciences 81(1): 3–14.

Walton J D. 1994.Deconstructing the cell wall.Plant Physiology 104(4): 1113–8. DOI: https://doi.org/10.1104/pp.104.4.1113

Yin Y, Mao X, Yang J C, Chen X, Mao F and Xu Y. 2012. dbCAN: A web resource for automated carbohydrate-active enzyme annotation. Nucleic Acids Research 40: 445–51. DOI: https://doi.org/10.1093/nar/gks479