Analysis of genetic diversity and population structure of the indigenous and exotic wild Malus species using ISSR markers

CHAVLESH KUMAR; S K SINGH; RAKESH SINGH; M K VERMA; K K PRAMANICK; SRIVASTAV SRIVASTAV; RAMESH KUMAR; J K VERMA; NARENDER NEGI

doi:10.56093/ijas.v89i7.91650

Authors

CHAVLESH KUMAR Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
S K SINGH Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
RAKESH SINGH Division of Genomic Resources, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
M K VERMA Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
K K PRAMANICK ICAR-IARI Regional Station, Amartara Cottage, Shimla
SRIVASTAV SRIVASTAV ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
RAMESH KUMAR Division of Genomic Resources, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
J K VERMA ICAR-IARI Regional Station, Amartara Cottage, Shimla
NARENDER NEGI ICAR-NBPGR Regional Station, Phagali, Shimla

https://doi.org/10.56093/ijas.v89i7.91650

Keywords:

Crabapples, Genetic diversity, Indigenous, ISSR markers, Wild Malus species

Abstract

The genetic diversity and population structure studies on 32 wild Malus species were conducted using the nine inter simple sequence repeat (ISSR) markers. The average value of diversity indices, viz. resolving power (Rp), polymorphic information content (PIC), effective multiplex ratio (EMR) and marker index (MI) of ISSR markers were 2.389, 0.388, 16.429 and 6.228, respectively, while the Jaccardâ€™s similarity coefficient ranged from 0.46 to 0.97. The cluster analysis divided the selected Malus species into two major clusters and principal coordinate analysis (PCoA) further reconfirmed the result of the cluster analysis. The first three axes of PCoA explained 43.49% variation and analysis of molecular variance (AMOVA) explained 16% variation between the indigenous and exotic Malus populations. The studied Malus species were genetically differentiated into four distinct populations which were revealed through the model based population structure analysis. Thus, the present investigation revealed substantial genetic diversity among the studied wild Malus species and existing genetic diversity could be valuable genetic resources for future apple improvement programme.

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