Identification of genotypes possessing GA responsive reduced height genes in bread wheat (Triticum aestivum)

PRAVEEN K YADAV; MONIKA A JOSHI; RAJBIR YADAV; MANJEET SINGH

doi:10.56093/ijas.v89i7.91699

Authors

PRAVEEN K YADAV Ph D Scholar, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
MONIKA A JOSHI Principal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
RAJBIR YADAV Principal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
MANJEET SINGH Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India

https://doi.org/10.56093/ijas.v89i7.91699

Keywords:

Coleoptile length, Dryland agriculture, Reduced height genes, SSR markers, Seedling traits

Abstract

The present study was conducted during years 2017-18 and 2018-19 involving 60 wheat genotypes including advance lines and released varieties. Based on the coleoptile length, these were categorised for coleoptile length as short (2.5-4.5 cm), medium (4.6-6.5 cm) and long (6.6-9 cm) respectively. Small and medium coleoptile length genotypes had comparable plant height (100.07cm and 99.29 cm, respectively) whereas, longer coleoptile genotypes were in general taller (103.78cm) numerically and for each class, the speed of germination was almost similar.A set of linked SSRs markers was used to screen the genotypes for molecular characterization based on GA responsive genes. In wheat, longer coleoptile length with reduced height is primarily controlled by GA responsive reduced height genes like Rht8, Rht12 etc., therefore, the material was screened to identify genotypes possessing GA responsive reduced height genes with available molecular markers. Molecular screening for Rht genes revealed five Rht8 positive genotypes having longer coleoptile length and good seedling vigour. Thus, Rht8 genes had positive effect on coleoptile length and had no detrimental effect on root length of seedling and provided better field emergence in field. Hence, these genotypes can be used for the development of variety for dryland agriculture and underconservation agriculture practices.

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References

Botwright T, RebetzkeG, Condon T andRichardsR.2001.The effect of rht genotype and temperature on coleoptile growth and dry matter partitioning in young wheat seedlings. Functional Plant Biology 28(5): 417–23. DOI: https://doi.org/10.1071/PP01010

Chan K Y. 2001. An overview of some tillage impact on the earthworm population abundance and diversity implication for functioning in soil. Soil and Tillage Research 57:197–1. DOI: https://doi.org/10.1016/S0167-1987(00)00173-2

Gasperini D, Greenland A, Hedden P, Dreos R, Harwood W and Griffiths S. 2012. Genetic and physiological analysis of Rht8 in bread wheat: an alternative source of semi-dwarfism with a reduced sensitivity to brassinosteroids. Journal of experimental botany 63(12): 4419. DOI: https://doi.org/10.1093/jxb/ers292

Grover G, Sharma A, Gill H S, Srivastava P and Bains N S .2018. Rht8 gene as an alternative dwarfing gene in elite Indian spring wheat cultivars. PloS one 13(6): e0199330. DOI: https://doi.org/10.1371/journal.pone.0199330

Liatukas Ž and Ruzgas V.2011.Coleoptile length and plant height of modern tall and semi-dwarf European winter wheat varieties. Acta Societatis Botanicorum Poloniae 80(3): 197. DOI: https://doi.org/10.5586/asbp.2011.018

Maguire J D. 1962. Speed of germination—Aid in selection and evaluation for seedling emergence and vigor I. Crop science 2(2): 176–7. DOI: https://doi.org/10.2135/cropsci1962.0011183X000200020033x

Mohan A, Schillinger W F and Gill K S. 2013.Wheat seedling emergence from deep planting depths and its relationship with coleoptile length. PLoS One 8(9): e73314. DOI: https://doi.org/10.1371/journal.pone.0073314

Mullis K, Faloona F, Scharf S, Saiki R K, Horn G T and Erlich H. 1986. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor Symposia on Quantitative Biology 51:263–73 DOI: https://doi.org/10.1101/SQB.1986.051.01.032

Tang Na, Jiang Y, He B R and Hu Y G. 2009. The effects of dwarfing genes (Rht-B1b, Rht-D1b, and Rht8) with different sensitivity to GA3 on the coleoptile length and plant height of wheat. Agricultural Sciences in China 8(9): 1028–38. DOI: https://doi.org/10.1016/S1671-2927(08)60310-7

Pandey M, Singh A K, DePauw R M, Bokore F E, Ellouze W, Knox R E and Cuthbert R D. 2015. Coleoptile length, gibberellin sensitivity, and plant height variation of durum wheat in Canada. Canadian Journal of Plant Science 95(6): 1259–64.

Rebetzke G J, Richards R A, Sirault X R R and Morrison A D. 2004. Genetic analysis of coleoptile length and diameter in wheat. Australian Journal of Agricultural Research 55(7):733–43. DOI: https://doi.org/10.1071/AR04037

Robbins A M.2009. Dwarfing genes in spring wheat: an agronomic comparison of Rht-B1, Rht-D1, and Rht8 (Doctoral dissertation, Montana State University-Bozeman, College of Agriculture).

Schmidt O, Clement R O and Donaldson G. 2003. Why do cereal-legume intercrops support large earthworm populations? Applied Soil Ecology 22:181–90. DOI: https://doi.org/10.1016/S0929-1393(02)00131-2