Wall bound phenolics and total antioxidants in stored seeds of soybean (Glycine max) genotypes

S P JEEVAN KUMAR; ASHUTOSH KUMAR; RAMESH K V; CHANDU SINGH; DINESH K AGARWAL; GOVIND PAL; MRINAL K KUCHLAN; RAJIV SINGH

doi:10.56093/ijas.v90i1.98557

Authors

S P JEEVAN KUMAR Scientist, ICAR-Indian Institute of Seed Science, Mau, Uttar Pradesh 275 103, India
ASHUTOSH KUMAR Research Associate, ICAR-Indian Institute of Seed Science, Mau, Uttar Pradesh 275 103, India
RAMESH K V Scientist, ICAR-Indian Institute of Seed Science, Mau, Uttar Pradesh 275 103, India
CHANDU SINGH Scientist, ICAR-Indian Institute of Seed Science, Mau, Uttar Pradesh 275 103, India
DINESH K AGARWAL Principal Scientist, ICAR-Indian Institute of Seed Science, Mau, Uttar Pradesh 275 103, India
GOVIND PAL Principal Scientist, ICAR-Indian Institute of Seed Science, Mau, Uttar Pradesh 275 103, India
MRINAL K KUCHLAN Scientist, ICAR-Indian Institute of Soybean Research, Indore, MP
RAJIV SINGH Principal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi

https://doi.org/10.56093/ijas.v90i1.98557

Keywords:

Antioxidants, Ferulic acid, Phenolics, Protocatechuic acid, Soybean

Abstract

Quality seed is the most important input for sustainable agricultural productivity. Recent studies highlight the role of antioxidants in scavenging the reactive oxygen species, which is an indicator for quality seed. In natural ageing conditions, reactive oxygen species accumulates in the seeds that lead to seed deterioration. Wall bound phenolics in seed coat are potential antioxidants that encounter the reactive oxygen species. Four soybean genotypes with different seed coat wall bound phenolics were studied in 2018 at ICAR-Indian Institute of Seed Science and found that the black coat seed such as kalitur and JS76-205 showed highest phenolic content (117.00 and 128.50 μg/mL) and antioxidant activity of 51.38 and 46.07 μg eq. ascorbic acid, respectively. On contrary, the harasoya (green coated seed) resulted lowest phenolic content 14.30 μg/mL, while in RAUS-05 (white coated seed) the antioxidant activity is 2.58 μg eq. ascorbic acid, which is significantly lower than the other genotypes. Further, the genotypes were evaluated for the seed quality parameters and observed that kalitur and JS76-205 showed higher germination percentage (80%) and vigor indices I (900.33) and II (35.01) than the other genotypes. These results substantiate the hypothesis that the seed coat wall bound phenolics with antioxidant properties could alleviate the reactive oxygen species, protects from mechanical and chemical damage and helps in enhancement of seed quality parameters.

Downloads

Download data is not yet available.

References

Agarwal D K, Billore S D, Sharma A N, Dupare B U and Srivastava S K. 2013. Soybean: introduction, improvement, and utilization in India-problems and prospects. Agricultural Research 2: 293–300. DOI: https://doi.org/10.1007/s40003-013-0088-0

Ali S, Liu Y, Ishaq M, Shah T, Abdullah Ilyas A and Din I U. 2017. Climate change and its impact on the yield of major food crops: Evidence from Pakistan. Foods Basel, Switzerland 6: 39. DOI: https://doi.org/10.3390/foods6060039

Campbell M M and Ellis B E. 1992. Fungal elicitor-mediated responses in pine cell cultures: cell wall-bound phenolics. Phytochemistry 31:737–42. DOI: https://doi.org/10.1016/0031-9422(92)80004-X

Capeleti I, Ferrarese M L L, Krzyzanowski F C and Ferrarese- Filho O. 2005. A new procedure for quantification of lignin in soybean (Glycine max (L.) Merrill) seed coat and their relationship with the resistance to mechanical damage. Seed Science and Technology 33: 511–5. DOI: https://doi.org/10.15258/sst.2005.33.2.25

Chandusingh, Sripathy K V, Kumar S P J, Naik B K, Pal G, Udayabhaskar K, Ramesh K V, Somasundaram G. 2017. Delineation of inheritance pattern of aleurone layer colour through chemical tests in rice. Rice 10: 48–55. DOI: https://doi.org/10.1186/s12284-017-0187-9

Chandusingh, Boraiah K M, Singh R K, Kumar S P J, Singh G, Chand R. 2018. Comparative study of floral biology using detergent and confirm self-incompatibility system in protogynous lines of Indian mustard (Brassica juncea). Indian Journal of Agricultural Sciences 88: 1190–7.

De Ascensao A R and Dubery I A. 2003. Soluble and wall-bound phenolics and phenolic polymers in Musa acuminata roots exposed to elicitors from Fusarium oxysporum f. sp. cubense. Phytochemistry 63: 679–86. DOI: https://doi.org/10.1016/S0031-9422(03)00286-3

Dona M, Balestrazzi A, Mondoni A, Rossi G, Ventura L, Buttafava A, Macovei A, Sabatini ME, Valassi A, Carbonera D. 2013. DNA profiling, telomere analysis and antioxidant properties as tools for monitoring ex situ seed longevity. Annals of botany 111:987–98. DOI: https://doi.org/10.1093/aob/mct058

Gomes M P and Garcia Q S. 2013.Reactive oxygen species and seed germination. Biologia 68: 351–7. DOI: https://doi.org/10.2478/s11756-013-0161-y

ISTA. 2008. International Rules for Seed Testing. Zurich. 31. pp. 288 (suppl.).

Khurana S, Venkataraman K, Hollingsworth A, Piche M and Tai T. 2013. Polyphenols: benefits to the cardiovascular system in health and in aging. Nutrients 5: 3779–827. DOI: https://doi.org/10.3390/nu5103779

Kumar A, Agarwal D K, Kumar S, Reddy Y M, Chintagunta A D, Saritha KV, Pal G and Kumar S J. 2019. Nutraceuticals derived from seed storage proteins: Implications for health wellness. Biocatalysis and Agricultural Biotechnology 17: 710–9. DOI: https://doi.org/10.1016/j.bcab.2019.01.044

Kumar S P J, Rajendra Prasad S, Kumar M, Singh C, Sinha A K and Pathak A. 2016. Seed quality markers: a review. Research & Reviews: Journal of Botanical Sciences 3: 13–17.

Kumar S P, Rajendra Prasad S, Banerjee R and Thammineni C. 2015. Seed birth to death: dual functions of reactive oxygen species in seed physiology. Annals of Botany 116: 663–8. DOI: https://doi.org/10.1093/aob/mcv098

Kumar S P J, Prasad S R, Banerjee R, Agarwal D K, Kulkarni K S, Ramesh K V. 2017. Green solvents and technologies for oil extraction from oilseeds. Chemistry Central Journal 11: 1–9. DOI: https://doi.org/10.1186/s13065-017-0238-8

Mhamdi B, Wannes W A, Sriti J, Jellali I, Ksouri R and Marzouk B. 2010. Effect of harvesting time on phenolic compounds and antiradical scavenging activity of Borago officinalis seed extracts. Industrial Crops and Products 31: 1–4. DOI: https://doi.org/10.1016/j.indcrop.2009.07.002

Pang Y, Ahmed S, Xu Y, Beta T, Zhu Z, Shao Y and Bao J. 2018. Bound phenolic compounds and antioxidant properties of whole grain and bran of white, red and black rice. Food Chemistry 240: 212–21. DOI: https://doi.org/10.1016/j.foodchem.2017.07.095

Panobianco M and Vieira R D. 1996. Electrical conductivity of soybean soaked seeds. I. Effect of genotype. Pesquisa Agro pecuária Brasileira 31:621–7.

Sano N, Rajjou L, North H M, Debeaujon I, Marion-Poll A and Seo M. 2015.Staying alive: molecular aspects of seed longevity. Plant and Cell Physiology 57: 660–74. DOI: https://doi.org/10.1093/pcp/pcv186

Sarangi S, Mandal C, Dutta S, Mukherjee P, Mondal R, Kumar S P J, Choudhury P R, Singh V P, Tripathi D K, Mandal A B. 2019. Microprojectile based particle bombardment in development of transgenic indica rice involving AmSOD gene to impart tolerance to salinity. Plant Gene 19: 100183. DOI: https://doi.org/10.1016/j.plgene.2019.100183

Singh A, Karmakar S, Jacob B S, Bhattacharya P, Kumar S P J, Banerjee R. 2015. Enzymatic polishing of cereal grains for improved nutrient retainment. Journal of Food Science and Technology 52: 3147–57. DOI: https://doi.org/10.1007/s13197-014-1405-8

Singh R P., Chintagunta A J, Agarwal D K, Kureel R S, Kumar S P J. 2019. Varietal replacement rate: Prospects and challenges for global food security. Global Food Security, 100324. DOI: https://doi.org/10.1016/j.gfs.2019.100324

Sinha A K, Agarwal D K, Kumar S J, Chaturvedi A and Tiwari T N. 2016. Novel technique for precluding hybrid necrosis in bread wheat. International Journal of Tropical Agriculture 34: 761–5.

Talai S and Sen-Mandi S. 2010. Seed vigour-related DNA marker in rice shows homology with acetyl CoA carboxylase gene. Acta Physiologiae Plantarum 32:153–67. DOI: https://doi.org/10.1007/s11738-009-0392-2

Tiwari T N, Kumar S P J, Tiwari A K, Agarwal D K. 2018. Seed coating in relation to minimizing the effects of seed ageing in rice (Oryza sativa L.). Journal of Rice Research 10 (2): 27–32.

Tully R E, Musgrave M E and Leopold A C. 1981.The seed coat as a control of imbibitional chilling injury. Crop Science 21: 312–7. DOI: https://doi.org/10.2135/cropsci1981.0011183X002100020026x

Vinutha K S, Prasad S R, Murthy P, Kumar S P J and Rame Gowda, R P. 2014. Influence of staggered sowing, planting ratio and subtending cob leaf clipping on seed quality parameters of maize. Seed Research 42: 91–97.

Yadav S, Bhatia V S and Guruprasad K N. 2006.Oxyradical accumulation and rapid deterioration of soybean seeds due to field weathering. Indian Journal of Biochemistry and Biophysics 43: 41–47.

Złotek U, Mikulska S, Nagajek M and Świeca M. 2016. The effect of different solvents and number of extraction steps on the polyphenol content and antioxidant capacity of basil leaves (Ocimum basilicum L.) extracts. Saudi Journal of Biological Sciences 23: 628–33. DOI: https://doi.org/10.1016/j.sjbs.2015.08.002