Variations in lipid profile of of the marine microalga Nannochloropsis salina in four different culture media


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Authors

  • RAJANI S. GANGA DEPARTMENT OF MARINE BIOLOGY, MICROBIOLOGY AND BIOCHEMISTRY, SCHOOL OF MARINE SCIENCES,COCHIN UNIVERSITY OF SCIENCE AND TECHNOLOGY,FINE ARTS AVENUE ROAD, KOCHI-16
  • R. Anandan
  • Aneykutty Joseph

https://doi.org/10.21077/ijf.2022.69.3.104044-10

Abstract

The present work envisages close monitoring of variations in lipid content and fatty acid profile of the marine microalga Nannochloropsis salina, by growing it in 4 different culture media. Pure cultures of microalga were collected from the marine hatchery complex of the ICAR-Central Marine Fisheries Research Institute, Kochi and cultured in four different media viz., f/2 medium (Guillard, 1975), Miquel’s medium (Miquel, 1892), Walne’s medium (Walne, 1974) and Chu#10 medium (Chu et al., 1942). Standard algal culture conditions viz, salinity 33 ppt, pH 8.0, culture room temperature of 25ºC, light intensity of 1500 lux with a photoperiod of 12 h and Light:Dark cycle of 12 h were maintained. Lipids extracted were subjected to gas chromatography to ascertain their fatty acid profile. Both Miquel’s and f/2 media produced the maximum amount of lipid content (40.2 and 40% respectively). Total saturated fatty acids ranged from 39.17% (Miquel’s medium) to 84.45% (Chu#10). For monounsaturated fatty acids, the highest value was obtained for Miquel’s medium (42.79%) and minimum for Chu#10 (10.55%). Polyunsaturated fatty acids accounted for over 23.50% of the total fatty acids in Walne’s medium followed by Miquel’s (18.04%), f/2(12.76%) and Chu#10(5.00%) media. One way analysis of variance of the data brought to light that the medium composition can influence the fatty acid pattern of microalgae significantly.

Keywords: Fish culture, Live feed, Micronutrients, Omega-3, Omega-6

Author Biography

  • RAJANI S. GANGA, DEPARTMENT OF MARINE BIOLOGY, MICROBIOLOGY AND BIOCHEMISTRY, SCHOOL OF MARINE SCIENCES,COCHIN UNIVERSITY OF SCIENCE AND TECHNOLOGY,FINE ARTS AVENUE ROAD, KOCHI-16

    MSc BOTANY-M.G. UTY., KOTTAYAM MPhil MICROBIOLOGY - M.G. UTY., KOTTAYAM BEd NATURAL SCIENCE - M.G. UTY., KOTTAYAM

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Submitted

2020-08-28

Published

2022-09-30

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Articles

How to Cite

GANGA, R. S., Anandan, R., & Joseph, A. (2022). Variations in lipid profile of of the marine microalga Nannochloropsis salina in four different culture media. Indian Journal of Fisheries, 69(3). https://doi.org/10.21077/ijf.2022.69.3.104044-10