Identification of Vibrio parahaemolyticus from farmed penaeid shrimp, Penaeus vannamei (Boone, 1931) by multiplex PCR targeting toxR and tlh genes
307 / 55
Authors
-
R. Ananda Raja
ICAR-Central Institute of Brackishwater Aquaculture (ICAR)
-
R. Sridhar
TANUVAS
-
C. Balachandran
TANUVAS
-
A. Palanisammi
TANUVAS
-
S. Ramesh
TANUVAS
-
K. Nagarajan
TANUVAS
-
S. Paulpandi
Scientist
Abstract
Targeted surveillance was conducted in 37 Penaeus vannamei farms in Tamil Nadu, India
to find out the prevalence of Vibrio parahaemolyticus (Vp) infections. Special emphasis was
given to screen the isolates for acute hepatopancreatic necrosis disease (AHPND). Bacteria
were isolated from haemolymph, stomach and hepatopancreas. Initial identifications
were carried out using the morphological, physiological and biochemical characteristics.
Vp isolates were further confirmed by a newly developed multiplex PCR targeting Vp specific
toxR and tlh genes. PCR screening was carried out for AHPND causing AP1, AP2, AP3
(pirAvp, Photorhabdus insect-related binary toxin gene) and AP4 (pirAvp and pirBvp) genes.
In addition, PCR was also performed for human pathogenic tdh and trh genes. The PCR
results revealed that there were 26 (35.14%) isolates affirmative for Vp specific toxR and tlh
genes and negative for AP1, AP2, AP3 and AP4 genes indicating that there was no AHPND
causing Vp strain among the isolates. The isolates were also negative for anthropozoonotic
tdh and trh genes. The multiplex PCR developed targeting Vp specific toxR and tlh genes
would be a useful technique for easy identification of Vp strains from shrimp farms.
Keywords:
Acute hepatopancreatic necrosis disease (AHPND), Early mortality syndrome (EMS), Multiplex PCR, Vibriosis, Vibrio parahaemolyticus (Vp)
Downloads
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2023 Indian Journal of Fisheries
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.How to Cite
