Effects of replacing fish meal with different levels of Lead tree (Leucaena leucocephala) leaf powder on growth, survival, digestive enzymes activity, muscle biochemical composition and texture of white-leg shrimp Penaeus vannamei Boone, 1931

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  • Tran Thi Phuong Lan Tra Vinh University
  • Tran Thi Hong To Tra Vinh University



This study investigated the effects of replacing fish meal with Leucaena leucocephala leaf powder on survival,
growth performance, digestive enzyme activities, biochemical body composition and muscle texture of the whiteleg shrimp (Penaeus vannamei Boone, 1931). Five different diets were examined, by replacing fish meal with
L. leucocephala leaf powder at 0, 5, 10, 15 and 20%, in triplicates. Shrimps were stocked in 0.5 m3 tanks @ 70 shrimps per
tank and fed on the experimental diets for a period of 60 days. The results showed that the survival rate (%), daily weight
gain (g day-1), specific growth rate (% day-1), feed intake (% fish-1 day-1), feed conversion ratio, protein efficiency ratio, and
protein retention were not significantly different (p>0,05) among the treatment groups. The values of α-amylase and pepsin
in the stomach and chymotrypsin (mU min-1 mg protein-1) in the intestine were not significantly different among treatments.
However, the value of α-amylase in the intestine was significantly higher in animals fed diets that replaced fish meal with
L. leucocephala leaf powder at 0, 5 and 10% compared to other treatments. The biochemical constituents (except for lipid)
and the texture (N) of shrimp meat were not significantly different among treatment groups. The results suggested that
L. leucocephala leaf powder could replace fish meal up to 20% in white-leg shrimp diets.

Keywords: Digestive enzyme, Growth, Leucaena leucocephala, White-leg shrimp

Author Biographies

  • Tran Thi Phuong Lan , Tra Vinh University

     Miss Tran Thi Phuong Lan

    Institution: School of Agriculture and Aquaculture, Tra Vinh University, Vietnam

    Responsibility: Lecturer and researcher; The main responsibilities are  developing AUN-aquaculture curriculum for Bachelor degree, providing lectures, tutoring and guiding students in research, practice, scientific discussion and vocational practice. The lectures mainly are on nutrient of aquatic animals

    Degree: Bachelor Degree in Aquaculture in 2002 and Master Degree in Aquaculture in 2008 at Can Tho University, Vietnam.

    Research experience: fish nutrient (the effect of different kind of feed on marble goby) and climate change (the effect of salinity and temperature on Pangasius fingerlings and snakehead fish culture).

    Research interest: Aquaculture nutrient, fish biology, reproduction of fish and the effect of climate changes in aquaculture.

  • Tran Thi Hong To, Tra Vinh University

    Dr. Tran Thi Hong To 

    Institution: School of Agriculture and Aquaculture, Tra Vinh University, Vietnam

    Position:  Vice Dean of School of Agriculture and Aquaculture

    Responsibility: Teaching, researching and faculty international support.

    Education: Bachelor degree in Aquaculture at Can Tho University, Vietnam in 2004; Master of Science (Sustainable Aquaculture) at Curtin University, Australia in 2011 and Doctor degree in Applied Veterinary Science (Specialization in epidemiology in aquaculture) at Tokyo University of Agriculture and Technology, Japan in 2019.

    Research experience: Aquaculture microbiology, fish biology, immune system of shrimp and fish and epidemiological study of shrimp and fish diseases.

    Awards: Australian Development Scholarship for Master Course from 2010-1011 in Curtin University, Perth, Western Australia; a short term scholarship to Bournemouth University, UK in 2012 funded by Erasmus Mundus.

    Research interest: aquaculture microbiology, aquaculture nutrient, aquatic diseases, immunostimulant, physiology and immune system of aquatic animals.


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How to Cite

Tran Thi, P. L., & Tran Thi, H. T. (2023). Effects of replacing fish meal with different levels of Lead tree (Leucaena leucocephala) leaf powder on growth, survival, digestive enzymes activity, muscle biochemical composition and texture of white-leg shrimp Penaeus vannamei Boone, 1931. Indian Journal of Fisheries, 70(1). https://doi.org/10.21077/ijf.2023.70.1.129063-09