Genetic diversity among three Indian populations of black tiger shrimp (Penaeus monodon Fabricious, 1798) using microsatellite DNA markers

Abstract

Genetic variation is an essential factor while selecting superior stocks for breeding programs. Eight microsatellite loci (TUZX pm2.27, TUZX pm2.28, TUZX pm2.36, TUZX pm2.69, TUZX pm4.84, TUZX pm4.87, TUZX pm6.1 and TUZX pm4.9) were used for molecular characterisation of Penaeus monodon collected from three different locations in India, viz., Kakinada and Chennai on the east coast and Mumbai on the west coast and all the loci were found to be highly polymorphic. The number of alleles and genotypes across the loci ranged from 5 to 23 and 42 to 98, respectively. The observed heterozygosity among the populations across the loci ranged from 0 to 0.1678. The overall FST value was found to be 0.021 (<0.05), which implies a low degree of population differentiation in the species. On the basis of pair-wise allelic differentiation and UPGMA dendrogram, the investigated samples from three locations were allocated in to two distinct populations composed of east coast and west coast populations.