Morphological and molecular evidence for range extension and taxonomic validation of Sperata aorides (Jerdon, 1849) in the Western Ghats, India

P. Bhavadharani; M. S. Sowmiya; Sajan John; R. Kumar; K. Kannan

doi:10.21077/ijf.2026.73.1.175049-06

Authors

P. Bhavadharani
M. S. Sowmiya
Sajan John
R. Kumar
K. Kannan KONGUNADU ARTS AND SCIENCE COLLEGE, COIMBATORE

https://doi.org/10.21077/ijf.2026.73.1.175049-06

Keywords:

Taxonomy, Diversity, Molecular and Classical Taxonomy

Abstract

Sperata aorides (Jerdon, 1849) is a commercially important freshwater catfish endemic to the Cauvery River basin, including its tributary, the Bhavani River. The present study report a range extension of the species to the Bharathappuzha River in Kerala, documenting its previously unrecorded distribution based on morphometric and molecular evidence. Specimens for the study were collected from different river systems in the Western Ghats, including the type locality at Erode, Tamil Nadu. Morphometric comparisons revealed minor variations between populations in head proportions, particularly in interorbital distance (19.5-24.5% vs 15.8-22.1% in HL) and eye diameter (12.0-17.3% vs 8.8-17.9% in HL). Mitochondrial cytochrome c oxidase subunit I (COI) gene sequencing confirmed species identity. Phylogenetic analysis showed that S. aorides forms a distinct clade, with low intraspecific genetic divergence (0.19%) between Cauvery and Bharathappuzha populations and higher divergence (4.61-9.85%) from congeners. These findings confirm the taxonomic validity of S. aorides and extend its known distribution to the Bharathappuzha River in the southern Western Ghats.

Keywords: Bharathappuzha, Catfishes, COI gene sequencing, Distribution, Tamil Nadu

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References

Acharya, A. P., Pavan-Kumar, A., Gireesh-Babu, P., Joshi, C. G., Chaudhari, A. and Krishna, G. 2019. Population genetics of Indian giant river-catfish, Sperata seenghala (Sykes, 1839) using microsatellite markers. Aquat. Living Resour., 32: 4. https://doi.org/10.1051/alr/2019002. DOI: https://doi.org/10.1051/alr/2019002

Ahyong, S., Boyko, C. B., Bernot, J., Brandao, S. N., Daly, M., De Grave, S., de Voogd, N. J., Gofas, S., Hernandez, F., Hughes, L., Neubauer, T. A., Paulay, G., Van der Meij, S., Boydens, B., Decock, W., Dekeyzer, S., Goharimanesh, M., Vandepitte, L., Vanhoorne, B. and Zullini, A. 2025. World Register of Marine Species database. https://www.marinespecies.org.

Armbruster, J. W. 2011. Global catfish biodiversity. Am. Fish. Soc. Symp., 77: 15–37.

Bhavadharani, P., Sowmiya, S., John, S., Kumar, R. and Kannan, K. 2025. Range extension and molecular confirmation of the critically endangered and endemic catfish Hemibagrus punctatus (Jerdon, 1849) (Siluriformes: Bagridae) from the southern Western Ghats. Indian J. Fish., 72 p. https://doi.org/10.21077/ijf.2025.72.3.168238-08. DOI: https://doi.org/10.21077/ijf.2025.72.3.168238-08

Cadrin, S. X. 2000. Advances in morphometric identification of fishery stocks. Rev. Fish Biol. Fish., 10: 91–112. DOI: https://doi.org/10.1023/A:1008939104413

Cuvier, G. and Valenciennes, A. 1840. Histoire naturelle des poissons. Tome quatorzième. Pitois Levrault.

Day, F. (1876 – 1878). The fishes of India: being a natural history of the fishes known to inhabit the seas and freshwaters of India, Burma and Ceylon. London. DOI: https://doi.org/10.5962/bhl.title.55567

Dumeril, A. M. C. 1856. Ichthyologie analytique ou essai dune classification naturelle des Poissons, alaide de tableaux synoptiques:(Extrait du tome XXVII des Mem. De lAcad. Des Sc.). Institut Imperial de France. DOI: https://doi.org/10.5962/bhl.title.16327

Ferraris, C. J. 2007. Checklist of catfishes, recent and fossil (Osteichthyes: Siluriformes), and catalogue of siluriform primary types. Zootaxa, 1418(1): 1–628. DOI: https://doi.org/10.11646/zootaxa.1418.1.1

Ferraris, C. J. and Runge, K. E. 1999. Revision of the South Asian bagrid catfish genus Sperata, with the description of a new species from Myanmar. Proc. Calif. Acad. Sci., 51(10): 397–424.

Fricke, R., Eschmeyer, W. N. and Fong, J. D. 2025. Eschmeyer’s Catalog of Fishes: Genera, Species by Family/Subfamily. http://researcharchive.calacade-my.org/research/ichthyology/catalog/ SpeciesByFamily.asp.

Froese, R. & Pauly, D. Editors. 2025. FishBase (version 04/2025).

https://www.fishbase.org.

Gatz, A. J. 1979. Ecological morphology of freshwater stream fishes.

Gupta, S. 2015. A review on Sperata aor (Hamilton, 1822), a popular food fish of Indian subcontinent. Sains Malays., 44(9): 1217–1222. https://doi.org/10.17576/jsm-2015-4409-01.s DOI: https://doi.org/10.17576/jsm-2015-4409-01

Hall, T. A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser., 41(41): 95–98.

Hamilton, F. 1822. An account of the fishes found in the river Ganges and its branches. Archibald Constable and company. DOI: https://doi.org/10.5962/bhl.title.59540

Hebert, P. D., Cywinska, A., Ball, S. L. and deWaard, J. R. 2003. Biological identifications through DNA barcodes. Proc. R. Soc. B, Proc. Biol. Sci., 270(1512): 313-321. https://doi.org/10.1098/rspb.2002.2218. DOI: https://doi.org/10.1098/rspb.2002.2218

Holly, M. 1939. Zur Nomenklatur der Siluridengattung Macrones, C. Duméril. Zool. Anz., 125: 143.

Iqbal, M. M., Alam, M. M., Kabir, M. A. and Palsson, S. 2018. Morphological and mitochondrial DNA variation revealed an undescribed lineage under the genus Sperata (Bagridae) in Bangladesh. Acta Zool., 99(2): 177–187. https://doi.org/10.1111/azo.12203. DOI: https://doi.org/10.1111/azo.12203

Jayaram, K. C. 1954. Siluroid Fishes of India, Burma and Ceylon. Part XIV. Fishes of the Genus Mystus Scopoli. Rec. Indian Mus., 51(4): 527-558. DOI: https://doi.org/10.26515/rzsi/v51/i4/1954/162110

Jayaram, K. C. 1966. Contributions to the study of Bagrid fishes (Siluroidea: Bagridae): A systematic account of the genera Rita Bleeker, Rama Bleeker, Mystus Scopoli, and Horabagrus Jayaram. Int. Rev. ges. Hydrobiol. Hydrogr., 51(3): 433–450. DOI: https://doi.org/10.1002/iroh.19660510303

Jerdon, T. C. 1849. On the fresh water fishes of southern India. Madras J. Lit. Sci., 15(2): 302-346.

Kumar, R. G., Charan, R., Krishnaprasoon, N. P. and Basheer, V. S. 2021. Catfishes of the genus Sperata (Pisces: Bagridae) in India. J. Fish Biol., 98(2); 456–469. https://doi.org/10.1111/jfb.14590. DOI: https://doi.org/10.1111/jfb.14590

Kumari, P., Pavan-Kumar, A., Kumar, G., Alam, A., Parhi, J., Gireesh-Babu, P., Chaudhari, A. and Krishna, G. 2017. Genetic diversity and demographic history of the giant river catfish Sperata seenghala inferred from mitochondrial DNA markers. Mitochondrial DNA Part A, 28(6): 920–926. https://doi.org/10.1080/24701394.2016.1209195. DOI: https://doi.org/10.1080/24701394.2016.1209195

Lakra, W. S., Singh, M., Goswami, M., Gopalakrishnan, A., Lal, K. K., Mohindra, V., Sarkar, U.K., Punia, P. P., Singh, K. V. and Bhatt, J. P. 2016. DNA barcoding Indian freshwater fishes. Mitochondrial DNA Part A, 27(6): 4510–4517. https://doi.org/10.3109/19401736.2015.1101540. DOI: https://doi.org/10.3109/19401736.2015.1101540

Li, L., Liang, H. W., Li, Z., Luo, X. Z., Hu, G. F., Zhang, Z. W., Zhu, Y. Y. and Zou, G. W. 2011. Sequence and phylogeny analysis of the complete mitochondrial genome of Pelteobagrus vachelli. Yi Chuan= Hereditas, 33(6): 627–635. https://doi.org/10.3724/sp.j.1005.2011.00627. DOI: https://doi.org/10.3724/SP.J.1005.2011.00627

Matthews W. J. 1988. Patterns in Freshwater Fish Ecology. Springer Science & Business Media.

Mirza, M. R., Nawaz, H. and Javed, M. N. 1992. A note on the fishes of genus Aorichthys Wu with the description of a new subspecies from Pakistan. Pak J Zool., 24(3): 211–213.

Modeel, S., Joshi, B. D., Yadav, A., Negi, T. and Negi, R. K. 2025. DNA-based identification of fishes: A review of barcoding methods and their efficiency assessment. Iran. J. Ichthyol., 12(1): 66–89. https://doi.org/10.22034/iji.v12i1.1014.

Mohanty, B. P., Paria, P., Das, D., Ganguly, S., Mitra, P., Verma, A., Sahoo, S., Mahanty, A., Aftabuddin, M. and Behera, B. K. 2012. Nutrient profile of giant river-catfish Sperata seenghala (Sykes). Natl. Acad. Sci. Lett., 35(3): 155–161. https://doi.org/10.1007/s40009-012-0014-1. DOI: https://doi.org/10.1007/s40009-012-0014-1

Sambrook, S. J., Russel, D. W., Janssen, K. A. and Irwin, N. J. 2001. Molecular Cloning, A laboratory Manual (3rd ed.). Cold Spring Harbor Laboratory Press.

Scopoli, J. A. 1777. Introductio ad historiam naturalem, sistens genera lapidum, plantarum et animalium hactenus detecta, caracteribus essentialibus donata, in tribus divisa, subinde ad leges naturae. Wolfgang Gerle. DOI: https://doi.org/10.5962/bhl.title.10827

Segaran, T. C., Azra, M. N., Piah, R. M., Lananan, F., Tellez-Isaias, G., Gao, H., Torsabo, D., Kari, Z. A. and Noordin, N. M. 2023. Catfishes: A global review of the literature. Heliyon, 9(9). https://doi.org/10.1016/j.heliyon.2023.e20081. DOI: https://doi.org/10.1016/j.heliyon.2023.e20081

Shen, Y., Guan, L., Wang, D. and Gan, X. 2016. DNA barcoding and evaluation of genetic diversity in Cyprinidae fish in the midstream of the Yangtze River. Ecology and Evolution 6(9): 2702–2713. https://doi.org/10.1002/ece3.2060. DOI: https://doi.org/10.1002/ece3.2060

Sowmiya, M. S., Bhavadharani, P., Felix, A. and Kannan, K. 2025. Unravelling taxonomic ambiguity of the Devario neilgherriensis (Teleostei: Danionidae) from the Southern Western Ghats, India through DNA barcoding and morphological approach. J. Ichthyol., 1-8 pp. https://doi. org/10.1134/S003294522460294X. DOI: https://doi.org/10.1134/S003294522460294X

Sykes, L. C. W. 1839. On the fishes of the Dukhun. Trans. Zool. Soc. London., 2(5): 349–378. https://doi.org/10.1111/j.1469-7998.1839.tb00029.x. DOI: https://doi.org/10.1111/j.1469-7998.1839.tb00029.x

Talwar, P. K. and Jhingran, A. G. 1991. Inland fishes of India and adjacent countries. Oxford and IBH Publishing.

Tamura, K., Stecher, G. and Kumar, S. 2021. MEGA11: Molecular evolutionary genetics analysis version 11. Mol. Biol. Evol., 38(7): 3022–3027. https://doi.org/10.1093/molbev/msab120. DOI: https://doi.org/10.1093/molbev/msab120

Tillett, B. J., Field, I. C., Bradshaw, C. J., Johnson, G., Buckworth, R. C., Meekan, M. G., and Ovenden, J. R. 2012. Accuracy of species identification by fisheries observers in a north Australian shark fishery. Fish. Res., 127: 109–115. https://doi.org/10.1016/j.fishres.2012.04.007. DOI: https://doi.org/10.1016/j.fishres.2012.04.007

Triantafyllidis, A., Bobori, D., Koliamitra, C., Gbandi, E., Mpanti, M., Petriki, O. and Karaiskou, N. 2011. DNA barcoding analysis of fish species diversity in four north Greek lakes. Mitochondrial DNA, 22(1): 37–42. https://doi.org/10.3109/19401736.2010.542242. DOI: https://doi.org/10.3109/19401736.2010.542242

Tripathi, S. D. 1996. Present status of breeding and culture of catfishes in South Asia. Aquat. Living Resour., 9(1): 219–228. https://doi.org/10.1051/alr:1996056. DOI: https://doi.org/10.1051/alr:1996056

Turan, C., Yalçin, S., Turan, F., Okur, E. and Akyurt, I. 2005. Morphometric comparisons of African catfish, Clarias gariepinus, populations in Turkey. Folia Zool., 54(1/2): 165.

Tyagi, K., Kumar, V., Singha, D., Chandra, K., Laskar, B. A., Kundu, S., Chakraborty, R. and Chatterjee, S. 2017. DNA barcoding studies on Thrips in India: Cryptic species, species complexes. Sci. Rep., 7: 1–14. DOI: https://doi.org/10.1038/s41598-017-05112-7

Ward, R. D., Zemlak, T. S., Innes, B. H., Last, P. R. and Hebert, P. D. 2005. DNA barcoding Australia's fish species. Philos. Trans. R. Soc. B, 360(1462): 1847-1857. DOI: https://doi.org/10.1098/rstb.2005.1716

Yadav, P., Kumar, A., Yadav, N., Bisht, M., Mital, A., Johnson, J. A., Hussain, S. A. and Gupta, S. 2022. Molecular evidence indicates the existence of multiple lineages of Sperata in Indian Rivers. DOI: https://doi.org/10.21203/rs.3.rs-1271404/v1