Detection of Vibrio parahaemolyticus from saltwater fish samples by Vp-toxR PCR

Dr. Anjay; Suresh Das; Ashok Kumar; Purushottam Kaushik; Baleshwari Kurmi

doi:10.21077/

Detection of Vibrio parahaemolyticus from saltwater fish samples by Vp-toxR PCR

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Authors

Dr. Anjay Bihar Agricultural University
Suresh Das IVRI,ERS, Kolkata
Ashok Kumar IVRI, Izatnagar
Purushottam Kaushik Bihar Veterinary College
Baleshwari Kurmi College of Veterinary Science & AH, Rewa

https://doi.org/10.21077/

Abstract

A total of sixty numbers of saltwater fish samples were collected and analysed by Vp-toxR PCR assay, directly from alkaline peptone water (APW) inoculated with samples and also by colony PCR from characteristic colonies on thiosulfate citrate bile salts sucrose (TCBS) agar. Vp-toxR PCR assay detected 5 (8.3%) samples positive for Vibrio parahaemolyticus directly from APW; whereas colony PCR detected 47 (78.3%) samples positive for the organism. Based on the results of the present study, it may be recommended to use colony PCR as a method suitable for detection of V. parahaemolyticus from saltwater fish samples.

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Author Biographies

Dr. Anjay, Bihar Agricultural University

Department of Veterinary Public Health
Assistant Professor
Suresh Das, IVRI,ERS, Kolkata

Eastern Regional Station, Kolkata
Principal Scientist
Ashok Kumar, IVRI, Izatnagar

Division of Veterinary Public Health
Principal Scientist & Head
Purushottam Kaushik, Bihar Veterinary College

Departmeant of Veterinary Public Health
Assistant Professor
Baleshwari Kurmi, College of Veterinary Science & AH, Rewa

Department of Veterinary Public Health
Assistant Professor

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Submitted

2012-09-19

Published

2013-03-30

Issue

Vol. 60 No. 1 (2013)

Section

Note

License

This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.

The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Anjay, D., Das, S., Kumar, A., Kaushik, P., & Kurmi, B. (2013). Detection of Vibrio parahaemolyticus from saltwater fish samples by Vp-toxR PCR. Indian Journal of Fisheries, 60(1). https://doi.org/10.21077/