Genetic diversity of the cyprinid fish Capoeta trutta (Heckel, 1843) populations from Euphrates and Tigris rivers in Turkey based on mtDNA COI sequences

Abstract

In this study, genetic diversity of Capoeta trutta (Heckel, 1843) populations from Euphrates and Tigris rivers in Turkey was evaluated based on gene sequence analysis of mitochondrial DNA cytochrome c oxidase subunit I (mtDNA COI) locus. Six polymorphic sites and seven haplotypes were detected in 47 samples collected from four populations viz., Adiyaman, Birecik, Bismil and Batman. The mean haplotype diversity (h) and nucleotide diversity (π) were calculated as h = 0,6420 and π = 0,00138 respectively. Pairwise FST statistics of different populations were found to be negative, low and were insignificant, indicating gene flow. AMOVA analysis showed Fst = 0.09865 and p = 0.00489, indicating that the populations were isolated. The results of Neutrality tests showed an increase in Adiyaman, Birecik and Bismil populations and a decline in Batman population, all values being statistically insignificant (p>0.05). Three haplotypes determined for mtDNA COI locus in the present study form important data set for genetic diversity of this species.